Effects of nano tantalum implants on inducing osteoblast proliferation and differentiation

Liu,Xinyu; Song,Xiaobin; Zhang,Peng; Zhu,Zhenkun; Xu,Xin

doi:10.3892/etm.2016.3801

December-2016 Volume 12 Issue 6

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December-2016 Volume 12 Issue 6

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Article Open Access

Effects of nano tantalum implants on inducing osteoblast proliferation and differentiation

Authors:
- Xinyu Liu
- Xiaobin Song
- Peng Zhang
- Zhenkun Zhu
- Xin Xu
View Affiliations / Copyright

Affiliations: Stomatological College of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Stomatology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Orthopedics, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250012, P.R. China

Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3541-3544
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Published online on: October 12, 2016

https://doi.org/10.3892/etm.2016.3801
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Abstract

In the present study, we examined the effects of nano tantalum (Ta) dental implants on inducing osteoblast proliferation and differentiation. The MG-63 osteoblasts were divided into 3 groups after recovery, passage and storage: i) Osteoblast culturing group (control group); ii) osteoblast and titanium (Ti) implant co-culturing group (Ti group); and iii) osteoblast and Ta implant co-culturing group (Ta group). After 7 days, a scanning electron microscope was used to observe the growth status, number and morphological changes of the cells on the surfaces of the materials. An MTT assay was used to detect cell proliferation after culturing for 1, 3 and 7 days. ELISA assay was used to detect the levels of alkaline phosphatase (ALP) after 1, 3 and 7 days. Western blot analysis was used to detect the expression levels of collagen type I (Col-1) and osteocalcin after 1, 3 and 7 days. There was significant cell spreading on the surfaces of Ti and of Ta after 7 days, flat and with many pseudopodia. Additionally, there were more cell components in the Ta group. Concurrently, cell proliferation in the Ti and Ta groups increased. There was also an increase in the level of ALP and the expression level of Col-1 over time. The indexes of the Ta group were more apparent than those of the Ti group at each time-point, and the differences were statistically significant (p<0.05). In conclusion, compared with Ti implants, Ta implants induced more osteoblast proliferation and differentiation.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of nano tantalum implants on inducing osteoblast proliferation and differentiation

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Abstract

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