Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2016 Volume 12 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2016 Volume 12 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus

  • Authors:
    • Hong Zhang
    • Haiqing Li
    • Yan Liu
    • Qingyan Li
    • Yufang Bi
    • Guiqing Fang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China, Department of Nursing, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China, Health Management Center, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China, Operation Room, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China, Department of Clinical Laboratory, Jinan Stomatological Hospital, Jinan, Shandong 250001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3571-3574
    |
    Published online on: October 14, 2016
       https://doi.org/10.3892/etm.2016.3805
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the study was to investigate the characteristic function of the upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus (MRSA). After separating the MRSA in clinic, the expression of miR-7 mRNA was tested by reverse transcription polymerase chain reaction. The overexpression, inhibition of miR-7, and control group were established by plasmid in vitro. Following transfection of the bacterial strain, the effect of β-lactam antibiotics in minimum inhibitory concentration (MIC) was observed using the microporous dilution method, and antibacterial effects in vitro were observed using the dynamic growth curve method. The expression of miR-7 in sensitive MRSA was upregulated distinctly, with significant difference (P<0.05). MIC and the number of bacteria in the miR-7 overexpression group significantly increased while the inhibition group decreased prominently, with significant difference (P<0.05). The control and null plasmid groups revealed no significant difference. In conclusion, miR-7 upregulated the antimicrobial activity of MRSA, and the intervention of its expression may become a possible antibacterial target.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Hale CM, Seabury RW, Steele JM, Darko W and Miller CD: Are vancomycin trough concentrations of 15 to 20 mg/L associated with increased attainment of an AUC/MIC ≥ 400 in patients with presumed MRSA infection? J Pharm Pract. 12:12–13. 2016.

2 

Aktaş Z, Satana D, Kayacan C, Can B, Gönüllü N and Küçükbasmacı O: Antibiotic susceptibility rates and beta-lactam resistance mechanisms of Pseudomonas aeruginosa strains. Mikrobiyol Bul. 46:386–397. 2012.(In Turkish). PubMed/NCBI

3 

Li H, Luo YF, Williams BJ, Blackwell TS and Xie CM: Structure and function of OprD protein in Pseudomonas aeruginosa: from antibiotic resistance to novel therapies. Int J Med Microbiol. 302:63–68. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Harrison EM, Ba X, Blane B, Ellington MJ, Loeffler A, Hill RL, Holmes MA and Peacock SJ: PBP2a substitutions linked to ceftaroline resistance in MRSA isolates from the UK. J Antimicrob Chemother. 71:268–269. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Ostojić M and Hukić M: Genotypic and phenotypic characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated on three different geography locations. Bosn J Basic Med Sci. 15:48–56. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Yang R, Zheng T, Cai X, Yu Y, Yu C, Guo L, Huang S, Zhu W, Zhu R, Yan Q, et al: Genome-wide analyses of amphioxus microRNAs reveal an immune regulation via miR-92d targeting C3. J Immunol. 190:1491–1500. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, et al: Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 18:268–281. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Datta J, Islam M, Dutta S, Roy S, Pan Q and Teknos TN: Suberoylanilide hydroxamic acid inhibits growth of head and neck cancer cell lines by reactivation of tumor suppressor microRNAs. Oral Oncol. 56:32–39. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Li MM, Addepalli B, Tu MJ, Chen QX, Wang WP, Limbach PA, LaSalle JM, Zeng S, Huang M and Yu AM: Chimeric microRNA-1291 biosynthesized efficiently in Escherichia coli is effective to reduce target gene expression in human carcinoma cells and improve chemosensitivity. Drug Metab Dispos. 43:1129–1136. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Li MM, Wang WP, Wu WJ, Huang M and Yu AM: Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells. Drug Metab Dispos. 42:1791–1795. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Jin W, Ibeagha-Awemu EM, Liang G, Beaudoin F, Zhao X and Guan L: Transcriptome microRNA profiling of bovine mammary epithelial cells challenged with Escherichia coli or Staphylococcus aureus bacteria reveals pathogen directed microRNA expression profiles. BMC Genomics. 15:1812014. View Article : Google Scholar : PubMed/NCBI

12 

Sun J, Aswath K, Schroeder SG, Lippolis JD, Reinhardt TA and Sonstegard TS: MicroRNA expression profiles of bovine milk exosomes in response to Staphylococcus aureus infection. BMC Genomics. 16:8062015. View Article : Google Scholar : PubMed/NCBI

13 

Zhou Y, Zhao R, Ma B, Gao H, Xue X, Qu D, Li M, Meng J, Luo X and Hou Z: Oligomerization of RNAIII-inhibiting peptide inhibits adherence and biofilm formation of methicillin-resistant Staphylococcus aureus in vitro and in vivo. Microb Drug Resist. 22:193–201. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Gupta RK, Luong TT and Lee CY: RNAIII of the Staphylococcus aureus agr system activates global regulator MgrA by stabilizing mRNA. Proc Natl Acad Sci USA. 112:14036–14041. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Ma B, Zhou Y, Li M, Yu Q, Xue X, Li Z, Da F, Hou Z and Luo X: RIP-V improves murine survival in a sepsis model by down-regulating RNAIII expression and α-hemolysin release of methicillin-resistant Staphylococcus aureus. Pharmazie. 70:81–87. 2015.PubMed/NCBI

16 

Zhang X, Zhu Q, Tian T, Zhao C, Zang J, Xue T and Sun B: Identification of RNAIII-binding proteins in Staphylococcus aureus using tethered RNAs and streptavidin aptamers based pull-down assay. BMC Microbiol. 15:1022015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Li H, Liu Y, Li Q, Bi Y and Fang G: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus. Exp Ther Med 12: 3571-3574, 2016.
APA
Zhang, H., Li, H., Liu, Y., Li, Q., Bi, Y., & Fang, G. (2016). Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus. Experimental and Therapeutic Medicine, 12, 3571-3574. https://doi.org/10.3892/etm.2016.3805
MLA
Zhang, H., Li, H., Liu, Y., Li, Q., Bi, Y., Fang, G."Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus". Experimental and Therapeutic Medicine 12.6 (2016): 3571-3574.
Chicago
Zhang, H., Li, H., Liu, Y., Li, Q., Bi, Y., Fang, G."Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3571-3574. https://doi.org/10.3892/etm.2016.3805
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Li H, Liu Y, Li Q, Bi Y and Fang G: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus. Exp Ther Med 12: 3571-3574, 2016.
APA
Zhang, H., Li, H., Liu, Y., Li, Q., Bi, Y., & Fang, G. (2016). Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus. Experimental and Therapeutic Medicine, 12, 3571-3574. https://doi.org/10.3892/etm.2016.3805
MLA
Zhang, H., Li, H., Liu, Y., Li, Q., Bi, Y., Fang, G."Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus". Experimental and Therapeutic Medicine 12.6 (2016): 3571-3574.
Chicago
Zhang, H., Li, H., Liu, Y., Li, Q., Bi, Y., Fang, G."Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3571-3574. https://doi.org/10.3892/etm.2016.3805
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team