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The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model

  • Authors:
    • Rong Dong
    • Buwei Yu
    • Lijiao Chen
    • Weifeng Yu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Eastern Hepatobiliary Hospital, The Second Military Medical University, Shanghai 200438, P.R. China, Department of Anesthesiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3583-3588
    |
    Published online on: October 14, 2016
       https://doi.org/10.3892/etm.2016.3807
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Abstract

Postoperative pain is a critical problem in clinical pain administration. Due to the unclear formation mechanism of postoperative pain, the treatment of postoperative pain is still in the symptomatic treatment stage and lacking satisfactory analgesic effect. Postoperative pain can be simulated by using a rat incision pain model. We observed changes in pain-related behavior of rats affected by the 5-hydroxytryptamine 2A receptor (5-HT2AR) agonist, TCB-2, and antagonist, ketanserin, through intrathecal delivery. The transcription and translation level of potassium-chloride cotransporter 2 (KCC2) in the spinal cord was also measured to investigate the role of the 5-HT2AR-KCC2 pathway in the mechanism of incision pain. Compared with the control group, rats in the incision pain group had decreased mechanical withdrawal threshold (MWT), with significant differences on day 1-7 after surgery, and significant decreases in thermal withdrawal latency (TWL) on days 1, 2, 3 and 6 (P<0.05). Compared with the incision + dimethyl sulfoxide (DMSO) group, MWT and TWL decreased in the incision + ketanserin group on day 1 and 2 (P<0.05). There was no significant difference detected in TWL of incision + TCB-2 group on day 1, while MWT increased significantly compared to the incision + DMSO group (P<0.05). Furthermore, the transcription and translation levels of KCC2 in the incision + ketanserin group decreased significantly in comparison to the incision + DMSO group (P<0.05), while an increase was detected in the incision + TCB-2 group (P<0.05). MWT and TWL decreased in the incision pain rats, accompanied with a decreased transcription and translation level of KCC2. Intrathecal delivery of the 5-HT2AR agonist, TCB-2, alleviated the decreased WMT and inhibited the decreased transcription and translation level of KCC2, while intrathecal delivery of the 5-HT2AR antagonist, ketanserin, aggravated the decreased WMT and transcription and translation levels of KCC2, suggesting the involement of the 5-HT2AR-KCC2 pathway in the formation mechanism of incision pain in rats.
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Copy and paste a formatted citation
Spandidos Publications style
Dong R, Yu B, Chen L and Yu W: The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model. Exp Ther Med 12: 3583-3588, 2016.
APA
Dong, R., Yu, B., Chen, L., & Yu, W. (2016). The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model. Experimental and Therapeutic Medicine, 12, 3583-3588. https://doi.org/10.3892/etm.2016.3807
MLA
Dong, R., Yu, B., Chen, L., Yu, W."The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model". Experimental and Therapeutic Medicine 12.6 (2016): 3583-3588.
Chicago
Dong, R., Yu, B., Chen, L., Yu, W."The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3583-3588. https://doi.org/10.3892/etm.2016.3807
Copy and paste a formatted citation
x
Spandidos Publications style
Dong R, Yu B, Chen L and Yu W: The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model. Exp Ther Med 12: 3583-3588, 2016.
APA
Dong, R., Yu, B., Chen, L., & Yu, W. (2016). The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model. Experimental and Therapeutic Medicine, 12, 3583-3588. https://doi.org/10.3892/etm.2016.3807
MLA
Dong, R., Yu, B., Chen, L., Yu, W."The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model". Experimental and Therapeutic Medicine 12.6 (2016): 3583-3588.
Chicago
Dong, R., Yu, B., Chen, L., Yu, W."The 5-HT2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3583-3588. https://doi.org/10.3892/etm.2016.3807
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