Clinical significance of lymphocyte subset changes in hemophagocytic lymphohistiocytosis of children

An,Qi; Wang,Yi; Hu,Shaoyan; Fang,Daihua; Xuan,Chengmin; Xu,Shumei; Jin,Mingwei; Ji,Qiang

doi:10.3892/etm.2016.3809

December-2016 Volume 12 Issue 6

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December-2016 Volume 12 Issue 6

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Article Open Access

Clinical significance of lymphocyte subset changes in hemophagocytic lymphohistiocytosis of children

Authors:
- Qi An
- Yi Wang
- Shaoyan Hu
- Daihua Fang
- Chengmin Xuan
- Shumei Xu
- Mingwei Jin
- Qiang Ji
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Affiliations: Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, P.R. China, Department of Hematology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221006, P.R. China

Copyright: © An et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3549-3552
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Published online on: October 17, 2016

https://doi.org/10.3892/etm.2016.3809
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Abstract

In order to examine the role of peripheral blood lymphocyte subsets on the diagnosis, treatment and prognosis of hemophagocytic lymphohistiocytosis (HLH), 30 affected children during the acute period of the disease and 30 healthy children within the same age range were selected to test their peripheral blood lymphocyte subsets using flow cytometry and compare these subsets. At the same time, the peripheral blood lymphocyte subsets of 20 children with complete remission from HLH were compared to those of 10 cases who succumbed to the disease. The proportion of CD3+ and CD8+ T cells were increased in children during the acute period. Additionally, the proportion of CD4+ T and CD3-CDl6+CD56+ natural killer (NK) cells and the ratio of CD4+/CD8+ cells were decreased in the same group of children, with the differences being statistical significance (P<0.05). The proportion of CD19+ B cells showed no differences in the affected and healthy groups. HLH children during the remission period had a higher proportion of CD3+ and CD8+ T cells than that in the control group, but the ratio of CD4+ T and CD4+/CD8+ were lower than that in the control group, with the differences being statistically significant (P<0.05). The proportion of CD19+ B cells and CD3-CD16+CD56+ NK cells revealed no significant difference between the two groups. In addition, regarding the proportion of CD3+, CD4+, CD8+ T, CD19+ B cells, CD3-CDl6+CD56+ NK cells and the ratio of CD4+/CD8+, and there were no significant differences. The results showed that HLH modifies the peripheral blood lymphocyte subsets and causes cellular immunity disorders. Thus, monitoring these dynamic changes can be useful in the diagnosis of HLH and evaluate the response to therapy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Clinical significance of lymphocyte subset changes in hemophagocytic lymphohistiocytosis of children

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