Functions of microRNA‑143 in the apoptosis, invasion and migration of nasopharyngeal carcinoma

  • Authors:
    • Jin‑Hui Chen
    • Rui Yang
    • Wei Zhang
    • Yong‑Ping Wang
  • View Affiliations

  • Published online on: October 27, 2016     https://doi.org/10.3892/etm.2016.3847
  • Pages: 3749-3755
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Abstract

Nasopharyngeal carcinoma (NPC) is a common cancer characterized by poor prognosis in areas of Southern China where it is endemic. microRNAs (miRNAs) are a class of naturally occurring small noncoding RNAs, some of which contribute to the initiation and development of cancer. The current study was designed to examine the expression level of miR‑143 in NPC tissues. The potential functional targets of miR‑143 involved in tumor apoptosis, invasion and migration were also investigated. Reverse transcription‑quantitative polymerase chain reaction was used to evaluate the expression levels of miR‑143 in clinical NPC specimens. Western blotting was used to explore the expression levels of extracellular signal regulated kinase (ERK)‑5, Kirsten rat sarcoma viral oncogene homolog (KRAS), caspase 3 and B‑cell lymphoma 2 (Bcl‑2) in CNE‑2Z cells following transfection with miR‑143. Significantly decreased expression levels of miR‑143 were observed in NPC tissues in comparison with matched normal nasopharyngeal tissues. Moreover, negative associations of miR‑143 with tumor invasion depth, as well as lymph node metastasis were found. The enforced expression of miR‑143 induced NPC cell apoptosis in addition to the suppression of growth, migration and invasion. The functions of miR‑143 in NPC are mediated, at least in part, by the inhibition of ERK‑5 activity and promotion of caspase 3 and KRAS expression. These findings suggest that miR‑143 may function as a tumor suppressor in the development and progression of NPC.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen JH, Yang R, Zhang W and Wang YP: Functions of microRNA‑143 in the apoptosis, invasion and migration of nasopharyngeal carcinoma. Exp Ther Med 12: 3749-3755, 2016
APA
Chen, J., Yang, R., Zhang, W., & Wang, Y. (2016). Functions of microRNA‑143 in the apoptosis, invasion and migration of nasopharyngeal carcinoma. Experimental and Therapeutic Medicine, 12, 3749-3755. https://doi.org/10.3892/etm.2016.3847
MLA
Chen, J., Yang, R., Zhang, W., Wang, Y."Functions of microRNA‑143 in the apoptosis, invasion and migration of nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 12.6 (2016): 3749-3755.
Chicago
Chen, J., Yang, R., Zhang, W., Wang, Y."Functions of microRNA‑143 in the apoptosis, invasion and migration of nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3749-3755. https://doi.org/10.3892/etm.2016.3847