Paeoniflorin attenuates hippocampal damage in a rat model of vascular dementia

Zhang,Ying; Wang,Li‑Li; Wu,Yan; Wang,Ning; Wang,Shang‑Ming; Zhang,Bin; Shi,Cui‑Ge; Zhang,Shu‑Cheng

doi:10.3892/etm.2016.3849

Paeoniflorin attenuates hippocampal damage in a rat model of vascular dementia

Authors:
- Ying Zhang
- Li‑Li Wang
- Yan Wu
- Ning Wang
- Shang‑Ming Wang
- Bin Zhang
- Cui‑Ge Shi
- Shu‑Cheng Zhang
View Affiliations

Affiliations: Research Center of Biomaterial and Novel Drug Delivery Systems, National Research Institute of Family Planning, Beijing 100081, P.R. China, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P.R. China, Department of Neurobiology, Beijing Institute of Basic Medical Sciences, Beijing 100850, P.R. China, Department of Cell Biology, National Research Institute of Family Planning, Beijing 100081, P.R. China
Published online on: October 27, 2016 https://doi.org/10.3892/etm.2016.3849
Pages: 3729-3734

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Paeoniflorn (PF), the principal bioactive component of Paeoniae radix prescribed in traditional Chinese medicine, possesses a wide range of biological effects and exhibits neuroprotective effects in numerous diseases. Previous studies have demonstrated that PF significantly attenuates memory impairment in rats with vascular dementia (VD). In the present study, a bilateral common carotid artery occlusion (BCCAO) rat model was used to explore the underlying mechanisms of PF. The expression levels of neuron‑specific enolase (NSE), S100β, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2 associated X protein, cytochrome c and brain‑derived neurotrophic factor (BDNF) in the hippocampus were measured by western blot analysis. The results showed that administration of PF for 28 days significantly decreased the expression levels of NSE and S100β, both sensitive markers for brain damage, in vascular dementia (VD) model rats. In addition, PF inhibited the initiation of apoptotic cell death and attenuated the decreased expression levels of BDNF induced by bilateral common carotid artery occlusion. These data confirm the neuroprotective effects of PF on VD and provide a novel insight into the long‑term use of PF as a potential treatment in the stages of early cognitive impairment in VD.

View Figures

View References

1	Ronnemaa E, Zethelius B, Lannfelt L and Kilander L: Vascular risk factors and dementia: 40-Year follow-up of a population-based cohort. Dement Geriatr Cogn Disord. 31:460–466. 2011. View Article : Google Scholar : PubMed/NCBI
2	Malouf R and Birks J: Donepezil for vascular cognitive impairment. Cochrane Database Syst Rev CD004395. 2004. View Article : Google Scholar
3	Ferri CP, Prince M, Brayne C, Brodaty H, Fratiglioni L, Ganguli M, Hall K, Hasegawa K, Hendrie H, Huang Y, et al: Global prevalence of dementia: A Delphi consensus study. Lancet. 366:2112–2117. 2005. View Article : Google Scholar : PubMed/NCBI
4	Yang S, Zhou GG, Liu H, et al: Portective effects of p38 MAPK inhibitor SB202190 against hippocampal apoptosis and spatial learning and memory deficits in a rat model of vascular dementia. Biomed Res Int,. 2013:2157982013. View Article : Google Scholar
5	Jellinger KA: The enigma of vascular cognitive disorder and vascular dementia. Acta Neuropathol. 113:349–388. 2007. View Article : Google Scholar : PubMed/NCBI
6	Levine DA and Langa KM: Vascular cognitive impairment: Disease mechanisms and therapeutic implications. Neurotherapeutics. 8:361–373. 2011. View Article : Google Scholar : PubMed/NCBI
7	Bennett S, Grant MM and Aldred S: Oxidative stress in vascular dementia and Alzheimer's disease: A common pathology. J Alzheimers Dis. 17:245–257. 2009.PubMed/NCBI
8	Wang J, Zhang HY and Tang XC: Cholinergic deficiency involved in vascular dementia: Possible mechanism and strategy of treatment. Acta Pharmacol Sin. 30:879–888. 2009. View Article : Google Scholar : PubMed/NCBI
9	Gong X, Ma M, Fan X, Li M, Liu Q, Liu X and Xu G: Down-regulation of IGF-1/IGF-1R in hippocampus of rats with vascular dementia. Neurosci Lett. 513:20–24. 2012. View Article : Google Scholar : PubMed/NCBI
10	Kasparova S, Brezova V, Valko M, et al: Study of the oxidative stress in a rat model of chronic brain hypoperfusion. Neurochem Int. 46:601–611. 2005. View Article : Google Scholar : PubMed/NCBI
11	Nizamutdinoval IT, Jim YC, Kim JS, et al: Paconol and paconiflorin, the main active principles of Paconiaalbiflora, protect the heart from myocardial ischemia/reperfusion injury in rats. Planta Med. 74:14–18. 2008. View Article : Google Scholar : PubMed/NCBI
12	Watanabe H: Candidates for cognitive enhancer extracted from medicinal plants: Paeoniflorin and tetramethylpyrazine. Behav Brain Res. 83:135–141. 1997. View Article : Google Scholar : PubMed/NCBI
13	Tang NY, Liu CH, Hsieh CT and Hsieh CL: The anti-inflammatory effect of paeoniflorin on cerebral infarction induced by ischemia-reperfusion injury in Sprague-Dawley rats. Am J Chin Med. 38:51–64. 2010. View Article : Google Scholar : PubMed/NCBI
14	Xiao L, Wang YZ, Liu J, Luo XT, Ye Y and Zhu XZ: Effects of paeoniflorin on the cerebral infarction, behavioral and cognitive impairments at the chronic stage of transient middle cerebral artery occlusion in rats. Life Sci. 78:413–420. 2005. View Article : Google Scholar : PubMed/NCBI
15	Zhang LG, Wang LJ, Shen QQ, et al: Paeoniflorin Improves Regional Cerebral Blood Flow and Suppresses Inflammatory Factors in the Hippocampus of Rats with Vascular Dementia. Chin J Integr Med. Epub ahead of print.
16	Institute of Laboratory Animal Research, Commission on Life Sciences, National Research Council, . Guide for the Care and Use of Laboratory Animals. 7th. National Academy Press; Washington, D.C.: pp. 56–66. 1996
17	D'Hooge R and De Devn PP: Applications of the Morris water maze in the study of learning and memory. Brain Res Brain Res Rev. 36:60–90. 2001. View Article : Google Scholar : PubMed/NCBI
18	Farkas E, Luiten PG and Bari F: Permanent, bilateral common carotid artery occlusion in the rat: A model for chronic cerebral hypoperfusion-related neurodegenerative diseases. Brain Res Rev. 54:162–180. 2007. View Article : Google Scholar : PubMed/NCBI
19	Berger RP, Dulani T, Adelson PD, Leventhal JM, Richichi R and Kochanek PM: Identification of inflicted traumatic brain injury in well-appearing infants using serum and cerebrospinal markers: A possible screening tool. Pediatrics. 117:325–332. 2006. View Article : Google Scholar : PubMed/NCBI
20	Gonçalves CA, Leite MC and Nardin P: Biological and methodological features of the measurement of S100B, a putative marker of brain injury. Clin Biochem. 41:755–763. 2008. View Article : Google Scholar : PubMed/NCBI
21	Mecocci P, Parnetti L, Romano G, Scarelli A, Chionne F, Cecchetti R, Polidori MC, Palumbo B, Cherubini A and Senin U: Serum anti-GFAP and anti-S100 autoantibodies in brain aging, Alzheimer's disease and vascular dementia. J Neuroimmunol. 57:165–170. 1995. View Article : Google Scholar : PubMed/NCBI
22	Parnetti L, Palumbo B, Cardinali L, Loreti F, Chionne F, Cecchetti R and Senin U: Cerebrospinal fluid neuron-specific enolase in Alzheimer's disease and vascular dementia. Neurosci Lett. 183:43–45. 1995. View Article : Google Scholar : PubMed/NCBI
23	Sun ZK, Ma XR, Jia YJ, et al: Effects of resveratrol on apoptosis in a rat model of vascular dementia. Exp Ther Med. 7:843–848. 2014.PubMed/NCBI
24	Min JJ, Huo XL, Xiang LY, et al: Protective effect of Dl-3n-butylphthalide on learning and memory impairment induced by chronic intermittent hypoxia-hypercapnia exposure. Sci Rep. 4:55552014. View Article : Google Scholar : PubMed/NCBI
25	Hwang L, Choi IY, Kim SE, Ko IG, Shin MS, Kim CJ, Kim SH, Jin JJ, Chung JY and Yi JW: Dexmedetomidine ameliorates intracerebral hemorrhage-induced memory impairment by inhibiting apoptosis and enhancing brain-derived neurotrophic factor expression in the rat hippocampus. Int J Mol Med. 31:1047–1056. 2013.PubMed/NCBI
26	Shimizu S, Narita M and Tsujimoto Y: Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC. Nature. 399:483–487. 1999. View Article : Google Scholar : PubMed/NCBI
27	Kluck RM, Bossy-Wetzel E, Green DR and Newmeyer DD: The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis. Science. 275:1132–1136. 1997. View Article : Google Scholar : PubMed/NCBI
28	Chuang DM: The antiapoptotic actions of mood stabilizers: molecular mechanisms and therapeutic potentials. Ann N Y Acad Sci. 1053:195–204. 2005. View Article : Google Scholar : PubMed/NCBI
29	Schinder AF and Poo M: The neurotrophin hypothesis for synaptic plasticity. Trends Neurosci. 23:639–645. 2000. View Article : Google Scholar : PubMed/NCBI
30	Mizuno M, Yamada K, Olariu A, Nawa H and Nabeshima T: Involvement of brain-derived neurotrophic factor in spatial memory formation and maintenance in a radial arm maze test in rats. J Neurosci. 20:7116–7121. 2000.PubMed/NCBI
31	Laske C, Stransky E, Leyhe T, Eschweiler GW, Maetzler W, Wittorf A, Soekadar S, Richartz E, Koehler N, Bartels M, et al: BDNF serum and CSF concentrations in Alzheimer's disease, normal pressure hydrocephalus and healthy controls. J Psychiatr Res. 41:387–394. 2007. View Article : Google Scholar : PubMed/NCBI
32	Murer MG, Yan Q and Raisman-Vozari R: Brain-derived neurotrophic factor in the control human brain and in Alzheimer's disease and Parkinson's disease. Prog Neurobiol. 63:71–124. 2001. View Article : Google Scholar : PubMed/NCBI