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Staining with two observational methods for the diagnosis of tuberculous meningitis

  • Authors:
    • Yueli Zou
    • Hui Bu
    • Li Guo
    • Yajuan Liu
    • Junying He
    • Xuedan Feng
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
    Copyright: © Zou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3934-3940
    |
    Published online on: November 2, 2016
       https://doi.org/10.3892/etm.2016.3859
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Abstract

Ziehl-Neelsen (Z-N) staining of cerebrospinal fluid (CSF) for acid‑fast bacilli (AFB) is the cornerstone of the laboratory diagnosis of tuberculous meningitis (TBM). However, the sensitivity of conventional Z‑N staining for the detection of AFB in CSF specimens is suboptimal. The present study aimed to compare the practicality of modified Z‑N staining with light microscopy and fluorescence microscopy in the same smear without auramine O. A total of 155 patients with 223 CSF specimens were enrolled and grouped according to the uniform case definition. The smears of each CSF specimen were subjected to modified Z‑N staining and then observed using a light microscope under transmitted light and under fluorescence with a green‑excitation wavelength in the same microscopic field. The results for different groups, inspection times, and prior to and following treatment were compared. Results indicated that the fuchsin‑stained AFB were visible as bright orange‑red fluorescing rods under fluorescence, or as red, lightly curved rods under transmitted light. The sensitivity of fluorescence microscopy was 96.2% while that of light microscopy was 84.6%. The positive rate of fluorescence microscopy was 79.2% prior to treatment compared with 61.7% post‑treatment. In the same microscopic field, a greater number of AFB were observed using fluorescence compared with transmitted light, and AFB that were not visible under transmitted light were clearly observed under fluorescence. Furthermore, transmitted light and fluorescence could be interchanged directly when equivocal smears were encountered. The combination of modified Z‑N staining and fluorescence microscopy without auramine O is sensitive and convenient for the diagnosis of TBM.
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1 

Yu HY, Hu FS, Xiang DR and Sheng JF: Clinical management of tuberculous meningitis: Experiences of 42 cases and literature review. Neurol Sci. 35:303–305. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Elkington PT: Tuberculosis: Time for a new perspective? J Infect. 66:299–302. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Zou Y, He J, Guo L, Bu H and Liu Y: Prediction of cerebrospinal fluid parameters for tuberculous meningitis. Diagn Cytopathol. 43:701–704. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Marais S, Thwaites G, Schoeman JF, Török ME, Misra UK, Prasad K, Donald PR, Wilkinson RJ and Marais BJ: Tuberculous meningitis: A uniform case definition for use in clinical research. Lancet Infect Dis. 10:803–812. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Thwaites GE: Advances in the diagnosis and treatment of tuberculous meningitis. Curr Opin Neurol. 26:295–300. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Chen P, Shi M, Feng GD, Liu JY, Wang BJ, Shi XD, Ma L, Liu XD, Yang YN, Dai W, et al: A highly efficient Ziehl-Neelsen stain: Identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid. J Clin Microbiol. 50:1166–1170. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Feng GD, Shi M, Ma L, Chen P, Wang BJ, Zhang M, Chang XL, Su XC, Yang YN, Fan XH, et al: Diagnostic accuracy of intracellular Mycobacterium tuberculosis detection for tuberculous meningitis. Am J Respir Crit Care Med. 189:475–481. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Ulrichs T, Lefmann M, Reich M, Morawietz L, Roth A, Brinkmann V, Kosmiadi GA, Seiler P, Aichele P, Hahn H, et al: Modified immunohistological staining allows detection of Ziehl-Neelsen-negative Mycobacterium tuberculosis organisms and their precise localization in human tissue. J Pathol. 205:633–640. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Yeager H Jr, Lacy J, Smith LR and LeMaistre CA: Quantitative studies of mycobacterial populations in sputum and saliva. Am Rev Respir Dis. 95:998–1004. 1967.PubMed/NCBI

10 

Thwaites GE, Chau TT and Farrar JJ: Improving the bacteriological diagnosis of tuberculous meningitis. J Clin Microbiol. 42:378–379. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Radhakrishnan VV and Mathai A: Correlation between the isolation of Mycobacterium tuberculosis and estimation of mycobacterial antigen in cisternal, ventricular and lumbar cerebrospinal fluids of patients with tuberculous meningitis. Indian J Pathol Microbiol. 36:341–347. 1993.PubMed/NCBI

12 

Marais BJ, Brittle W, Painczyk K, Hesseling AC, Beyers N, Wasserman E, van Soolingen D and Warren RM: Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum. Clin Infect Dis. 47:203–207. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Nhu NT, Heemskerk D, Thu do DA, Chau TT, Mai NT, Nghia HD, Loc PP, Ha DT, Merson L, Thinh TT, et al: Evaluation of GeneXpert MTB/RIF for diagnosis of tuberculous meningitis. J Clin Microbiol. 52:226–233. 2014. View Article : Google Scholar : PubMed/NCBI

14 

World Health Organization, . Global Tuberculosis Control: WHO Report 2010. World Health Organization; Geneva: 2010

15 

Thwaites GE, van Toorn R and Schoeman J: Tuberculous meningitis: More questions, still too few answers. Lancet Neurlo. 12:999–1010. 2013. View Article : Google Scholar

16 

Zakham F, Akrim M, El Mzibri M, Benjouad A, El Aouad R and Ennaji MM: Rapid screening and diagnosis of tuberculosis: A real challenge for the mycobacteriologist. Cell Mol Biol (Noisy-le-grand). 58:(Suppl). S1632–S1640. 2012.

17 

Shapiro HM and Hänscheid T: Fuchsin fluorescence in Mycobacterium tuberculosis: The Ziehl-Neelsen stain in a new light. J Microbiol Methods. 74:119–120. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Varea M, Clavel A, Doiz O, Castillo FJ, Rubio MC and Gómez-Lus R: Fuchsin fluorescence and autofluorescence in Cryptosporidium, Isospora and Cyclospora oocysts. Int J Parasitol. 28:1881–1883. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Steingart KR, Henry M, Ng V, Hopewell PC, Ramsay A, Cunningham J, Urbanczik R, Perkins M, Aziz MA and Pai M: Fluorescence versus conventional sputum smear microscopy for tuberculosis: A systematic review. Lancet Infect Dis. 6:570–581. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Kivihya-Ndugga LE, van Cleeff MR, Githui WA, Nganga LW, Kibuga DK, Odhiambo JA and Klatser PR: A comprehensive comparison of Ziehl-Neelsen and fluorescence microscopy for the diagnosis of tuberculosis in a resource-poor urban setting. Int J Tuberc Lung Dis. 7:1163–1171. 2003.PubMed/NCBI

21 

Ulukanligil M, Aslan G and Tasçi S: A comparative study on the different staining methods and number of specimens for the detection of acid fast bacilli. Mem Inst Oswaldo Cruz. 95:855–858. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Hänscheid T: The future looks bright: Low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae. Trans R Soc Trop Med Hyg. 102:520–521. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Lehman LG, Ngapmen Yamadji AL, Ngo Sack F and Bilong Bilong CF: The CyScope® fluorescence microscope, a reliable tool for tuberculosis diagnosis in resource-limited settings. Am J Trop Med Hyg. 83:906–908. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Habtamu M, van den Boogaard J, Ndaro A, Buretta R, Irongo CF, Lega DA, Nyombi BM and Kibiki GS: Light-emitting diode with various sputum smear preparation techniques to diagnose tuberculosis. Int J Tuberc Lung Dis. 16:402–407. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Ba F and Rieder HL: A comparison of fluorescence microscopy with the Ziehl-Neelsen technique in the examination of sputum for acid-fast bacilli. Int J Tuberc Lung Dis. 3:1101–1105. 1999.PubMed/NCBI

26 

Prasanthi K and Kumari AR: Efficacy of fluorochrome stain in the diagnosis of pulmonary tuberculosis co-infected with HIV. Indian J Med Microbiol. 23:179–181. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Xia H, Song YY, Zhao B, Kam KM, O'Brien RJ, Zhang ZY, Sohn H, Wang W and Zhao YL: Multicentre evaluation of Ziehl-Neelsen and light-emitting diode fluorescence microscopy in China. Int J Tuberc Lung Dis. 17:107–112. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Willett HP and Thacore H: Formation of spheroplasts of Mycobacterium tuberculosis by lysozyme in combination with certain enzymes of rabbit peritoneal monocytes. Can J Microbiol. 13:481–487. 1967. View Article : Google Scholar : PubMed/NCBI

29 

Ye HP and Xu HP: Research about L-form of Mycobacterium tuberculosis. Jiang Xi Zhong Yi Xue Yuan Xue Bao. 12:92–93. 2000.(In Chinese).

30 

Sohn H, Sinthuwattanawibool C, Rienthong S and Varma JK: Fluorescence microscopy is less expensive than Ziehl-Neelsen microscopy in Thailand. Int J Tuberc Lung Dis. 13:266–268. 2009.PubMed/NCBI

31 

Silva DR, Valentini DF Jr, Müller AM, de Almeida CP and Pde T Dalcin: Pulmonary tuberculosis and lung cancer: Simultaneous and sequential occurrence. J Bras Pneumol. 39:484–489. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Coussens LM and Werb Z: Inflammation and cancer. Nature. 420:860–867. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Aibar-Arregui MA, de Escalante-Yangüela B, Tejero-Juste C and Martín-Fortea MP: Mixed meningoencephalitis caused by Mycobacterium tuberculosis and varicella zoster virus. Rev Neurol. 48:91–93. 2009.(In Spanish). PubMed/NCBI

34 

Cicenas S and Vencevicius V: Lung cancer in patients with tuberculosis. World J Surg Oncol. 5:222007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zou Y, Bu H, Guo L, Liu Y, He J and Feng X: Staining with two observational methods for the diagnosis of tuberculous meningitis. Exp Ther Med 12: 3934-3940, 2016.
APA
Zou, Y., Bu, H., Guo, L., Liu, Y., He, J., & Feng, X. (2016). Staining with two observational methods for the diagnosis of tuberculous meningitis. Experimental and Therapeutic Medicine, 12, 3934-3940. https://doi.org/10.3892/etm.2016.3859
MLA
Zou, Y., Bu, H., Guo, L., Liu, Y., He, J., Feng, X."Staining with two observational methods for the diagnosis of tuberculous meningitis". Experimental and Therapeutic Medicine 12.6 (2016): 3934-3940.
Chicago
Zou, Y., Bu, H., Guo, L., Liu, Y., He, J., Feng, X."Staining with two observational methods for the diagnosis of tuberculous meningitis". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3934-3940. https://doi.org/10.3892/etm.2016.3859
Copy and paste a formatted citation
x
Spandidos Publications style
Zou Y, Bu H, Guo L, Liu Y, He J and Feng X: Staining with two observational methods for the diagnosis of tuberculous meningitis. Exp Ther Med 12: 3934-3940, 2016.
APA
Zou, Y., Bu, H., Guo, L., Liu, Y., He, J., & Feng, X. (2016). Staining with two observational methods for the diagnosis of tuberculous meningitis. Experimental and Therapeutic Medicine, 12, 3934-3940. https://doi.org/10.3892/etm.2016.3859
MLA
Zou, Y., Bu, H., Guo, L., Liu, Y., He, J., Feng, X."Staining with two observational methods for the diagnosis of tuberculous meningitis". Experimental and Therapeutic Medicine 12.6 (2016): 3934-3940.
Chicago
Zou, Y., Bu, H., Guo, L., Liu, Y., He, J., Feng, X."Staining with two observational methods for the diagnosis of tuberculous meningitis". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3934-3940. https://doi.org/10.3892/etm.2016.3859
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