Unenhanced areas revealed by contrast-enhanced abdominal ultrasonography with SonazoidTM potentially correspond to colorectal cancer

Tomizawa,Minoru; Togashi,Mizuki; Shinozaki,Fuminobu; Hasegawa,Rumiko; Shirai,Yoshinori; Noritake,Midori; Matsuoka,Yukie; Kainuma,Hiroaki; Iwasaki,Yasuji; Fugo,Kazunori; Motoyoshi,Yasufumi; Sugiyama,Takao; Yamamoto,Shigenori; Kishimoto,Takashi; Ishige,Naoki

doi:10.3892/etm.2016.3868

Unenhanced areas revealed by contrast-enhanced abdominal ultrasonography with SonazoidTM potentially correspond to colorectal cancer

Authors:
- Minoru Tomizawa
- Mizuki Togashi
- Fuminobu Shinozaki
- Rumiko Hasegawa
- Yoshinori Shirai
- Midori Noritake
- Yukie Matsuoka
- Hiroaki Kainuma
- Yasuji Iwasaki
- Kazunori Fugo
- Yasufumi Motoyoshi
- Takao Sugiyama
- Shigenori Yamamoto
- Takashi Kishimoto
- Naoki Ishige
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Affiliations: Department of Gastroenterology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284‑0003, Japan, Department of Clinical Laboratory, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284‑0003, Japan, Department of Radiology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284‑0003, Japan, Department of Surgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido City, Chiba, 284‑0003, Japan, Department of Molecular Pathology, Chiba University Graduate School of Medicine, Chiba City, Chiba 260‑8670, Japan, Department of Neurology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284‑0003, Japan, Department of Rheumatology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284‑0003, Japan, Department of Pediatrics, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284‑0003, Japan, Department of Neurosurgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Yotsukaido City, Chiba 284‑0003, Japan
Published online on: November 3, 2016 https://doi.org/10.3892/etm.2016.3868
Pages: 4012-4016

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Abstract

The present study investigated the potential utility of contrast‑enhanced abdominal ultrasonography (CEUS), using SonazoidTM, in colorectal cancer (CRC). Three patients were subjected to CEUS with SonazoidTM. Surgical specimens were immunostained for CD31. Numbers of blood vessels positive for CD31 were analyzed in each of five fields at x400 magnification and averaged to determine blood vessel density. Blood vessel density was compared between non‑tumorous and tumorous areas. Prior to the administration of SonazoidTM, CRC was illustrated as irregular‑shaped wall thickening. One minute after the administration of SonazoidTM, the majority of the thickened wall was enhanced, while some parts of the thickened wall remained unenhanced. Blood vessel densities of non‑tumorous and tumorous areas in patient two were 25.2±2.5 and 5.2±1.1 (P<0.0001). Blood vessel densities of non‑tumorous and tumorous areas in patient three were 19.0±3.1 and 2.2±0.8 (P<0.0001). Tumorous areas of CRC were not enhanced 1 min after the administration of SonazoidTM. Blood vessel density was lower in tumorous areas compared with non‑tumorous areas, as evidenced by immunohistochemistry for CD31. These findings suggest that CEUS may be useful for the determination of the extent of CRC.

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