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Article

Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells

  • Authors:
    • Meng‑Yu Cheng
    • Hu Liu
    • Tie‑Mei Zhang
    • Jian‑Ying Xu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Dayi Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi 030032, P.R. China, Second Department of Respiratory Medicine, Jimo City People's Hospital, Qingdao, Shandong 266200, P.R. China
  • Pages: 4168-4174
    |
    Published online on: November 4, 2016
       https://doi.org/10.3892/etm.2016.3872
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Abstract

Chronic obstructive pulmonary disease (COPD) is a common respiratory disease, in which adiponectin may serve an important role. The present study investigated the role of adiponectin in the apoptotic and damaging effect of cigarette smoke extract (CSE) on human bronchial epithelial cells (16HBECs). An MTT assay showed that CSE significantly inhibited the proliferation of 16HBECs (F=1808.88, P<0.01). The 16HBECs were treated with different concentrations of high molecular weight (HMW) adiponectin and globular domain (gAd) adiponectin and it was observed that HMW and gAd dose‑dependently inhibited the expression of tumor necrosis factor (TNF)‑α and interleukin (IL)‑8, and the generation of 4‑hydroxy‑nonenal and reactive oxygen species (ROS) in 16HBECs, thereby blocking the upregulating effect of CSE on these factors. However, the inhibitory effect of gAd on TNF‑α and IL‑8 expression was stronger compared with that of HMW, but the suppressing effect of HMW on ROS production was superior compared with that of gAd. Further testing of apoptosis indicated that CSE and HMW promoted the apoptosis of 16HBECs. However, such effects of HMW declined with an increase in concentration. In contrast, gAd showed an inhibitory effect on apoptosis and inhibited the occurrence of CSE‑induced apoptosis in a dose‑dependent manner. Therefore, the present study demonstrated that different forms of adiponectin may have different mechanisms of action, suggesting that further exploration of their effects may open a new avenue for the treatment of COPD.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng MY, Liu H, Zhang TM and Xu JY: Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells. Exp Ther Med 12: 4168-4174, 2016.
APA
Cheng, M., Liu, H., Zhang, T., & Xu, J. (2016). Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells. Experimental and Therapeutic Medicine, 12, 4168-4174. https://doi.org/10.3892/etm.2016.3872
MLA
Cheng, M., Liu, H., Zhang, T., Xu, J."Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells". Experimental and Therapeutic Medicine 12.6 (2016): 4168-4174.
Chicago
Cheng, M., Liu, H., Zhang, T., Xu, J."Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4168-4174. https://doi.org/10.3892/etm.2016.3872
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng MY, Liu H, Zhang TM and Xu JY: Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells. Exp Ther Med 12: 4168-4174, 2016.
APA
Cheng, M., Liu, H., Zhang, T., & Xu, J. (2016). Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells. Experimental and Therapeutic Medicine, 12, 4168-4174. https://doi.org/10.3892/etm.2016.3872
MLA
Cheng, M., Liu, H., Zhang, T., Xu, J."Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells". Experimental and Therapeutic Medicine 12.6 (2016): 4168-4174.
Chicago
Cheng, M., Liu, H., Zhang, T., Xu, J."Different forms of adiponectin reduce the apoptotic and damaging effect of cigarette smoke extract on human bronchial epithelial cells". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4168-4174. https://doi.org/10.3892/etm.2016.3872
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