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Article

C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells

  • Authors:
    • Junxia Wang
    • Yanning Li
    • Mingzhi Xu
    • Dandan Li
    • Yu Wang
    • Jinsheng Qi
    • Kunyu He
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology, Hebei Key Lab of Laboratory Animal Science, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Biochemistry, Hebei Key Laboratory of Medical Biotechnology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China
  • Pages: 4142-4146
    |
    Published online on: November 4, 2016
       https://doi.org/10.3892/etm.2016.3873
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Abstract

Insufficient matrix metalloproteinase (MMP)‑9 and MMP‑2 is considered to be a contributor of extracellular matrix (ECM) accumulation in diabetic nephropathy (DN). C‑peptide can reverse fibrosis, thus exerting a beneficial effect on DN. Whether C‑peptide induces MMP‑9 and MMP‑2 to reverse ECM accumulation is not clear. In the present study, in order to determine ECM metabolism, rat mesangial cells were treated with high glucose (HG) and C‑peptide intervention, then the early and late effects of C‑peptide on HG‑affected MMP‑9 and MMP‑2 were evaluated. Firstly, it was confirmed that HG mainly suppressed MMP‑9 expression levels. Furthermore, C‑peptide treatment induced MMP‑9 expression at 6 h and suppressed it at 24 h, revealing the early dual effects of C‑peptide on MMP‑9 expression. Subsequently, significant increase in MMP‑9 expression at 72, 96 and 120 h C‑peptide treatment was observed. These changes in MMP‑9 protein content confirmed its expression changes following late C‑peptide treatment. Furthermore, at 96 and 120 h C‑peptide treatment reversed the HG‑inhibited MMP‑9 secretion, further indicating the late induction effect of C‑peptide on MMP‑9. The present results demonstrated that C‑peptide exerted a late induction effect on MMP‑9 in HG‑stimulated rat mesangial cells, which may be associated with the underlying mechanism of C‑peptide's reversal effects on DN.
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1 

Kato M and Natarajan R: Diabetic nephropathy-emerging epigenetic mechanisms. Nat Rev Nephrol. 10:517–530. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Zhang Y, Xiao HQ, Wang Y, Yang ZS, Dai LJ and Xu YC: Differential expression and therapeutic efficacy of microRNA-346 in diabetic nephropathy mice. Exp Ther Med. 10:106–112. 2015.PubMed/NCBI

3 

Zhang L, Zhang J, Liu X, Liu S and Tian J: Tribbles 3 regulates the fibrosis cytokine TGF-β1 through ERK1/2-MAPK signaling pathway in diabetic nephropathy. J Immunol Res. 2014:2403962014. View Article : Google Scholar : PubMed/NCBI

4 

Miller CG, Pozzi A, Zent R and Schwarzbauer JE: Effects of high glucose on integrin activity and fibronectin matrix assembly by mesangial cells. Mol Biol Cell. 25:2342–2350. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Galliera E, Tacchini L and Romanelli MM Corsi: Matrix metalloproteinases as biomarkers of disease: Updates and new insights. Clin Chem Lab Med. 53:349–355. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Tauro M, McGuire J and Lynch CC: New approaches to selectively target cancer-associated matrix metalloproteinase activity. Cancer Metastasis Rev. 33:1043–1057. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Piperi C and Papavassiliou AG: Molecular mechanisms regulating matrix metalloproteinases. Curr Top Med Chem. 12:1095–1112. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Fukami K, Yamagishi S, Coughlan MT, Harcourt BE, Kantharidis P, Thallas-Bonke V, Okuda S, Cooper ME and Forbes JM: Ramipril inhibits AGE-RAGE-induced matrix metalloproteinase-2 activation in experimental diabetic nephropathy. Diabetol Metab Syndr. 6:862014. View Article : Google Scholar : PubMed/NCBI

9 

Zhong Y, Zhang X, Cai X, Wang K, Chen Y and Deng Y: Puerarin attenuated early diabetic kidney injury through down-regulation of matrix metalloproteinase 9 in streptozotocin-induced diabetic rats. PLoS One. 9:e856902014. View Article : Google Scholar : PubMed/NCBI

10 

Lu H, Cao X, Zhang H, Sun G, Fan G, Chen L and Wang S: Imbalance between MMP-2, 9 and TIMP-1 promote the invasion and metastasis of renal cell carcinoma via SKP2 signaling pathways. Tumour Biol. 35:9807–9813. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Sun H, Ge N, Shao M, Cheng X, Li Y, Li S and Shen J: Lumbrokinase attenuates diabetic nephropathy through regulating extracellular matrix degradation in Streptozotocin-induced diabetic rats. Diabetes Res Clin Pract. 100:85–95. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Thrailkill KM, Bunn R Clay and Fowlkes JL: Matrix metalloproteinases: Their potential role in the pathogenesis of diabetic nephropathy. Endocrine. 35:1–10. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Lewandowski KC, Banach E, Bieńkiewicz M and Lewiński A: Matrix metalloproteinases in type 2 diabetes and non-diabetic controls: Effects of short-term and chronic hyperglycaemia. Arch Med Sci. 7:294–303. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Li SY, Huang PH, Yang AH, Tarng DC, Yang WC, Lin CC, Chen JW, Schmid-Schönbein G and Lin SJ: Matrix metalloproteinase-9 deficiency attenuates diabetic nephropathy by modulation of podocyte functions and dedifferentiation. Kidney Int. 86:358–369. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Potier M, Elliot SJ, Tack I, Lenz O, Striker GE, Striker LJ and Karl M: Expression and regulation of estrogen receptors in mesangial cells: Influence on matrix metalloproteinase-9. J Am Soc Nephrol. 12:241–251. 2001.PubMed/NCBI

16 

Bhatt MP, Lim YC, Hwang J, Na S, Kim YM and Ha KS: C-peptide prevents hyperglycemia-induced endothelial apoptosis through inhibition of reactive oxygen species-mediated transglutaminase 2 activation. Diabetes. 62:243–253. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Al-Rasheed NM, Willars GB and Brunskill NJ: C-peptide signals via Galpha i to protect against TNF-alpha-mediated apoptosis of opossum kidney proximal tubular cells. J Am Soc Nephrol. 17:986–995. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Hills CE, Brunskill NJ and Squires PE: C-peptide as a therapeutic tool in diabetic nephropathy. Am J Nephrol. 31:389–397. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Wahren J, Kallas A and Sima AA: The clinical potential of C-peptide replacement in type 1 diabetes. Diabetes. 61:761–772. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Samnegård B, Jacobson SH, Jaremko G, Johansson BL and Sjöquist M: Effects of C-peptide on glomerular and renal size and renal function in diabetic rats. Kidney Int. 60:1258–1265. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Huang DY, Richter K, Breidenbach A and Vallon V: Human C-peptide acutely lowers glomerular hyperfiltration and proteinuria in diabetic rats: A dose-response study. Naunyn Schmiedebergs Arch Pharmacol. 365:67–73. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Sun W, Gao X, Zhao X, Cui D and Xia Q: Beneficial effects of C-peptide on renal morphology in diabetic rats. Acta Biochim Biophys Sin (Shanghai). 42:893–899. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Ishii T, Fukano K, Shimada K, Kamikawa A, Okamatsu-Ogura Y, Terao A, Yoshida T, Saito M and Kimura K: Proinsulin C-peptide activates α-enolase: Implications for C-peptide-cell membrane interaction. J Biochem. 152:53–62. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Luppi P, Geng X, Cifarelli V, Drain P and Trucco M: C-peptide is internalized in human endothelial smooth muscle cells via early endosomes. Diabetologia. 52:2218–2228. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Lindahl E, Nyman U, Zaman F, Palmberg C, Cascante A, Shafqat J, Takigawa M, Sävendahl L, Jörnvall H and Joseph B: Proinsulin C-peptide regulates ribosomal RNA expression. J Biol Chem. 285:3462–3469. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Li Y, Zhao M, Li B and Qi J: Dynamic localization and functional implications of C-peptide might for suppression of iNOS in high glucose-stimulated rat mesangial cells. Mol Cell Endocrinol. 381:255–260. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Li Y, Liu D, Liu Y, Li E, Wang H, Liu K and Qi J: Protein nitration promotes inducible nitric oxide synthase transcription mediated by NF-κB in high glucose-stimulated human lens epithelial cells. Mol Cell Endocrinol. 370:78–86. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Lehners A, Lange S, Niemann G, Rosendahl A, Meyer-Schwesinger C, Oh J, Stahl R, Ehmke H, Benndorf R, Klinke A, et al: Myeloperoxidase deficiency ameliorates progression of chronic kidney disease in mice. Am J Physiol Renal Physiol. 307:F407–F417. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Kuno Y, Iyoda M, Shibata T, Hirai Y and Akizawa T: Sildenafil, a phosphodiesterase type 5 inhibitor, attenuates diabetic nephropathy in non-insulin-dependent otsuka long-evans tokushima fatty rats. Br J Pharmacol. 162:1389–1400. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Samnegård B, Jacobson SH, Jaremko G, Johansson BL, Ekberg K, Isaksson B, Eriksson L, Wahren J and Sjöquist M: C-peptide prevents glomerular hypertrophy and mesangial matrix expansion in diabetic rats. Nephrol Dial Transplant. 20:532–538. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Li Y, Xu M, Li D, Wang Y, Qi J and He K: C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells. Exp Ther Med 12: 4142-4146, 2016.
APA
Wang, J., Li, Y., Xu, M., Li, D., Wang, Y., Qi, J., & He, K. (2016). C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells. Experimental and Therapeutic Medicine, 12, 4142-4146. https://doi.org/10.3892/etm.2016.3873
MLA
Wang, J., Li, Y., Xu, M., Li, D., Wang, Y., Qi, J., He, K."C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells". Experimental and Therapeutic Medicine 12.6 (2016): 4142-4146.
Chicago
Wang, J., Li, Y., Xu, M., Li, D., Wang, Y., Qi, J., He, K."C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4142-4146. https://doi.org/10.3892/etm.2016.3873
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Li Y, Xu M, Li D, Wang Y, Qi J and He K: C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells. Exp Ther Med 12: 4142-4146, 2016.
APA
Wang, J., Li, Y., Xu, M., Li, D., Wang, Y., Qi, J., & He, K. (2016). C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells. Experimental and Therapeutic Medicine, 12, 4142-4146. https://doi.org/10.3892/etm.2016.3873
MLA
Wang, J., Li, Y., Xu, M., Li, D., Wang, Y., Qi, J., He, K."C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells". Experimental and Therapeutic Medicine 12.6 (2016): 4142-4146.
Chicago
Wang, J., Li, Y., Xu, M., Li, D., Wang, Y., Qi, J., He, K."C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4142-4146. https://doi.org/10.3892/etm.2016.3873
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