C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells

Wang,Junxia; Li,Yanning; Xu,Mingzhi; Li,Dandan; Wang,Yu; Qi,Jinsheng; He,Kunyu

doi:10.3892/etm.2016.3873

C‑peptide exhibits a late induction effect on matrix metallopeptidase‑9 in high glucose‑stimulated rat mesangial cells

Authors:
- Junxia Wang
- Yanning Li
- Mingzhi Xu
- Dandan Li
- Yu Wang
- Jinsheng Qi
- Kunyu He
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Affiliations: Department of Molecular Biology, Hebei Key Lab of Laboratory Animal Science, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Biochemistry, Hebei Key Laboratory of Medical Biotechnology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China
Published online on: November 4, 2016 https://doi.org/10.3892/etm.2016.3873
Pages: 4142-4146

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Abstract

Insufficient matrix metalloproteinase (MMP)‑9 and MMP‑2 is considered to be a contributor of extracellular matrix (ECM) accumulation in diabetic nephropathy (DN). C‑peptide can reverse fibrosis, thus exerting a beneficial effect on DN. Whether C‑peptide induces MMP‑9 and MMP‑2 to reverse ECM accumulation is not clear. In the present study, in order to determine ECM metabolism, rat mesangial cells were treated with high glucose (HG) and C‑peptide intervention, then the early and late effects of C‑peptide on HG‑affected MMP‑9 and MMP‑2 were evaluated. Firstly, it was confirmed that HG mainly suppressed MMP‑9 expression levels. Furthermore, C‑peptide treatment induced MMP‑9 expression at 6 h and suppressed it at 24 h, revealing the early dual effects of C‑peptide on MMP‑9 expression. Subsequently, significant increase in MMP‑9 expression at 72, 96 and 120 h C‑peptide treatment was observed. These changes in MMP‑9 protein content confirmed its expression changes following late C‑peptide treatment. Furthermore, at 96 and 120 h C‑peptide treatment reversed the HG‑inhibited MMP‑9 secretion, further indicating the late induction effect of C‑peptide on MMP‑9. The present results demonstrated that C‑peptide exerted a late induction effect on MMP‑9 in HG‑stimulated rat mesangial cells, which may be associated with the underlying mechanism of C‑peptide's reversal effects on DN.

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