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Article

Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages

  • Authors:
    • Renchao Yu
    • Yuexia Lv
    • Juanling Wang
    • Nana Pan
    • Rui Zhang
    • Xiaxia Wang
    • Haichu Yu
    • Lijuan Tan
    • Yunhe Zhao
    • Bo Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, P.R. China, Clinical Skill Training Center, People's Hospital of Weifang, Weifang, Shandong 261041, P.R. China, Department of Cardiology, Central Hospital of Zibo, Zibo, Shandong 255036, P.R. China
  • Pages: 4113-4120
    |
    Published online on: November 8, 2016
       https://doi.org/10.3892/etm.2016.3884
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Abstract

Intake of a high dosage of baicalin has previously been shown to attenuate hyperlipidemia induced by a high-fat diet. Baicalin functions as an activator of peroxisome proliferator‑activated receptor‑γ (PPAR‑γ), which is the key regulator of reverse cholesterol transport (RCT). The present study aimed to test the hypothesis that baicalin could promote cholesterol efflux in macrophages through activating PPAR‑γ. Phorbol 12‑myristate 13‑acetate‑stimulated THP‑1 cells were treated with oxidized low‑density lipoprotein and (3H)‑cholesterol for 24 h, and the effects of baicalin on cholesterol efflux were evaluated in the presence of apolipoprotein A‑1 (ApoA‑1), or high‑density lipoprotein subfraction 2 (HDL2) or subfraction 3 (HDL3). The expression levels of scavenger receptor class B type I (SR‑BI), PPAR‑γ and liver X receptor‑α (LXRα) were detected and specific inhibitors or activators of SR‑BI, PPAR‑γ and LXRα were applied to investigate the mechanism. Treatment of THP‑1 macrophages with baicalin significantly accelerated HDL‑mediated, but not ApoA‑1‑mediated cholesterol efflux. However, baicalin treatment increased the expression of SR‑BI at the mRNA and protein levels in a dose‑ and time‑dependent manner, and pre‑treatment with the SR‑BI inhibitor BLT‑1 and SR‑BI small interfering RNA significantly inhibited baicalin‑induced cholesterol efflux. Furthermore, baicalin increased the expression of PPAR‑γ and LXRα, and the application of specific agonists and inhibitors of PPAR‑γ and LXRα changed the expression of SR‑BI, as well as cholesterol efflux. It may be concluded that baicalin induced cholesterol efflux from THP‑1 macrophages via the PPAR‑γ/LXRα/SR‑BI pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yu R, Lv Y, Wang J, Pan N, Zhang R, Wang X, Yu H, Tan L, Zhao Y, Li B, Li B, et al: Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages. Exp Ther Med 12: 4113-4120, 2016.
APA
Yu, R., Lv, Y., Wang, J., Pan, N., Zhang, R., Wang, X. ... Li, B. (2016). Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages. Experimental and Therapeutic Medicine, 12, 4113-4120. https://doi.org/10.3892/etm.2016.3884
MLA
Yu, R., Lv, Y., Wang, J., Pan, N., Zhang, R., Wang, X., Yu, H., Tan, L., Zhao, Y., Li, B."Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages". Experimental and Therapeutic Medicine 12.6 (2016): 4113-4120.
Chicago
Yu, R., Lv, Y., Wang, J., Pan, N., Zhang, R., Wang, X., Yu, H., Tan, L., Zhao, Y., Li, B."Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4113-4120. https://doi.org/10.3892/etm.2016.3884
Copy and paste a formatted citation
x
Spandidos Publications style
Yu R, Lv Y, Wang J, Pan N, Zhang R, Wang X, Yu H, Tan L, Zhao Y, Li B, Li B, et al: Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages. Exp Ther Med 12: 4113-4120, 2016.
APA
Yu, R., Lv, Y., Wang, J., Pan, N., Zhang, R., Wang, X. ... Li, B. (2016). Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages. Experimental and Therapeutic Medicine, 12, 4113-4120. https://doi.org/10.3892/etm.2016.3884
MLA
Yu, R., Lv, Y., Wang, J., Pan, N., Zhang, R., Wang, X., Yu, H., Tan, L., Zhao, Y., Li, B."Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages". Experimental and Therapeutic Medicine 12.6 (2016): 4113-4120.
Chicago
Yu, R., Lv, Y., Wang, J., Pan, N., Zhang, R., Wang, X., Yu, H., Tan, L., Zhao, Y., Li, B."Baicalin promotes cholesterol efflux by regulating the expression of SR‑BI in macrophages". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4113-4120. https://doi.org/10.3892/etm.2016.3884
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