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Article

Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine

  • Authors:
    • Sang Weon Lee
    • Sung Jin Kim
    • Hyungwoo Kim
    • Dongki Yang
    • Hyun Jung Kim
    • Byung Joo Kim
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnam 50612, Republic of Korea, Division of Pharmacology, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam 50612, Republic of Korea, Department of Physiology, Gachon University College of Medicine, Incheon, Gyeonggi 22332, Republic of Korea, Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam 50612, Republic of Korea
  • Pages: 327-334
    |
    Published online on: December 7, 2016
       https://doi.org/10.3892/etm.2016.3963
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Abstract

Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster‑induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m‑PM) on ICC pacemaker activities were examined using the whole‑cell patch‑clamp technique. ICC pacemaker membrane potentials were depolarized by m‑PM in a concentration dependent manner in current clamp mode. 4‑Diphenylacetoxy‑N‑methyl‑piperidine methiodide, which is a muscarinic 3 (M3) receptor antagonist, was able to block m‑PM‑induced pacemaker potential increases, whereas methoctramine, which is a muscarinic 2 (M2) receptor antagonist, was not. When 1 mM guanosine diphosphate β‑5 was present in the pipette solution, m‑PM induced slight pacemaker depolarization. Following pretreatment in bath solution of Ca2+‑free solution or a Ca2+‑ATPase inhibitor in endoplasmic reticulum, the pacemaker currents were inhibited. Furthermore, pretreatment with PD98059, SB203580 or SP600125, which is a c‑jun NH2‑terminal kinase inhibitor, blocked m‑PM‑induced ICC potential depolarization. Furthermore, m‑PM inhibited transient receptor potential melastatin (TRPM) 7 channels, but did not affect Ca2+‑activated Cl‑ channels. These results suggest that m‑PM is able to modulate pacemaker potentials through the muscarinic M3 receptor, via G‑protein and external and internal Ca2+, in a mitogen‑activated protein kinase and TRPM7‑dependent manner. Therefore, m‑PM may provide a basis for the development of a novel gastroprokinetic agent.
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Copy and paste a formatted citation
Spandidos Publications style
Lee SW, Kim SJ, Kim H, Yang D, Kim HJ and Kim BJ: Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine. Exp Ther Med 13: 327-334, 2017.
APA
Lee, S.W., Kim, S.J., Kim, H., Yang, D., Kim, H.J., & Kim, B.J. (2017). Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine. Experimental and Therapeutic Medicine, 13, 327-334. https://doi.org/10.3892/etm.2016.3963
MLA
Lee, S. W., Kim, S. J., Kim, H., Yang, D., Kim, H. J., Kim, B. J."Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine". Experimental and Therapeutic Medicine 13.1 (2017): 327-334.
Chicago
Lee, S. W., Kim, S. J., Kim, H., Yang, D., Kim, H. J., Kim, B. J."Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine". Experimental and Therapeutic Medicine 13, no. 1 (2017): 327-334. https://doi.org/10.3892/etm.2016.3963
Copy and paste a formatted citation
x
Spandidos Publications style
Lee SW, Kim SJ, Kim H, Yang D, Kim HJ and Kim BJ: Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine. Exp Ther Med 13: 327-334, 2017.
APA
Lee, S.W., Kim, S.J., Kim, H., Yang, D., Kim, H.J., & Kim, B.J. (2017). Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine. Experimental and Therapeutic Medicine, 13, 327-334. https://doi.org/10.3892/etm.2016.3963
MLA
Lee, S. W., Kim, S. J., Kim, H., Yang, D., Kim, H. J., Kim, B. J."Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine". Experimental and Therapeutic Medicine 13.1 (2017): 327-334.
Chicago
Lee, S. W., Kim, S. J., Kim, H., Yang, D., Kim, H. J., Kim, B. J."Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine". Experimental and Therapeutic Medicine 13, no. 1 (2017): 327-334. https://doi.org/10.3892/etm.2016.3963
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