Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats

Luo,Li; Guo,Kaihua; Fan,Wenguo; Lu,Yinghong; Chen,Lizhi; Wang,Yang; Shao,Yijia; Wu,Gongxiong; Xu,Jie; Lü,Lanhai

doi:10.3892/etm.2016.4016

Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats

Authors:
- Li Luo
- Kaihua Guo
- Wenguo Fan
- Yinghong Lu
- Lizhi Chen
- Yang Wang
- Yijia Shao
- Gongxiong Wu
- Jie Xu
- Lanhai Lü
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Affiliations: Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology and Institute of Stomatological Research, Hospital of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Published online on: December 30, 2016 https://doi.org/10.3892/etm.2016.4016
Pages: 645-650

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Abstract

Niche astrocytes have been reported to promote neuronal differentiation through juxtacrine signaling. However, the effects of astrocytes on neuronal differentiation following ischemic stroke are not fully understood. In the present study, transplanted astrocytes and neural stem cells (NSCs) were transplanted into the ischemic striatum of transient middle cerebral artery occlusion (MCAO) model rats 48 h following surgery. It was observed that the co‑transplantation of astrocytes and NSCs resulted in a higher ratio of survival and proliferation of the transplanted NSCs, and neuronal differentiation, in MCAO rats compared with NSC transplantation alone. These results demonstrate that the co‑administration of astrocytes promotes the survival and neuronal differentiation of NSCs in the ischemic brain. These results suggest that the co‑transplantation of astrocytes and NSCs is more effective than NSCs alone in the production of neurons following ischemic stroke in rats.