MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1

Zhao,Pengfei; Wang,Shaochun; Zhou,Yingjie; Zheng,Huailiang; Zhao,Gang

doi:10.3892/etm.2017.4052

MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1

Authors:
- Pengfei Zhao
- Shaochun Wang
- Yingjie Zhou
- Huailiang Zheng
- Gang Zhao
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Affiliations: Luoyang Orthopedic‑Traumatological Hospital, Luoyang, Henan 471002, P.R. China
Published online on: January 17, 2017 https://doi.org/10.3892/etm.2017.4052
Pages: 1127-1132

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Abstract

The aims of the present study were to examine the expression of transforming growth factor (TGF)-β1 and microRNA (miR)‑185 in the bone tissue, blood and cerebrospinal fluid of patients with spinal cord injuries and to evaluate the regulation of spinal cord injuries by miR‑185. A total of 44 patients with spinal cord injuries induced by thoracolumbar spine compression fractures, who were hospitalized at Luoyang Orthopedic‑Traumatological Hospital between June 2012 and February 2015 were enrolled in the present study. Among the patients enrolled, 18 underwent surgery between 1 and 7 days following fracture, and 26 patients underwent surgery between 8 and 14 days following fracture. Bone tissue, peripheral blood and cerebrospinal fluid were subsequently harvested from patients for analysis. Reverse transcription‑quantitative polymerase chain reaction was performed to determine the expression of miR‑185 and TGF‑β1 mRNA. Western blotting was performed to evaluate TGF‑β1 protein expression in bone tissue and ELISA was employed to quantify TGF‑β1 protein expression in the blood and cerebrospinal fluid. TGF‑β1 mRNA and protein levels in bone tissue, blood and cerebrospinal fluid from patients who underwent surgery 8‑14 days post‑fracture were significantly higher than those who underwent surgery 1‑7 days post‑fracture (P<0.05). By contrast, miR‑185 levels were significantly lower in bone tissue, blood and cerebrospinal fluid from patients who underwent surgery 8‑14 days post‑fracture compared with those who underwent surgery 1‑7 days post‑fracture (P<0.05). The results of the present study desmonstrate that the upregulation of TGF‑β1 in the bone tissue, blood and cerebrospinal fluid of patients with spinal cord injuries induced by thoracolumbar spine compression fractures is correlated with the downregulation of miR‑185. Furthermore, miR‑185 may target TGF‑β1, affecting its transcription and translation, indicating that it serves an important role in spinal cord injuries induced by thoracolumbar spine compression fractures.

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