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Article

MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1

  • Authors:
    • Pengfei Zhao
    • Shaochun Wang
    • Yingjie Zhou
    • Huailiang Zheng
    • Gang Zhao
  • View Affiliations / Copyright

    Affiliations: Luoyang Orthopedic‑Traumatological Hospital, Luoyang, Henan 471002, P.R. China
  • Pages: 1127-1132
    |
    Published online on: January 17, 2017
       https://doi.org/10.3892/etm.2017.4052
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Abstract

The aims of the present study were to examine the expression of transforming growth factor (TGF)-β1 and microRNA (miR)‑185 in the bone tissue, blood and cerebrospinal fluid of patients with spinal cord injuries and to evaluate the regulation of spinal cord injuries by miR‑185. A total of 44 patients with spinal cord injuries induced by thoracolumbar spine compression fractures, who were hospitalized at Luoyang Orthopedic‑Traumatological Hospital between June 2012 and February 2015 were enrolled in the present study. Among the patients enrolled, 18 underwent surgery between 1 and 7 days following fracture, and 26 patients underwent surgery between 8 and 14 days following fracture. Bone tissue, peripheral blood and cerebrospinal fluid were subsequently harvested from patients for analysis. Reverse transcription‑quantitative polymerase chain reaction was performed to determine the expression of miR‑185 and TGF‑β1 mRNA. Western blotting was performed to evaluate TGF‑β1 protein expression in bone tissue and ELISA was employed to quantify TGF‑β1 protein expression in the blood and cerebrospinal fluid. TGF‑β1 mRNA and protein levels in bone tissue, blood and cerebrospinal fluid from patients who underwent surgery 8‑14 days post‑fracture were significantly higher than those who underwent surgery 1‑7 days post‑fracture (P<0.05). By contrast, miR‑185 levels were significantly lower in bone tissue, blood and cerebrospinal fluid from patients who underwent surgery 8‑14 days post‑fracture compared with those who underwent surgery 1‑7 days post‑fracture (P<0.05). The results of the present study desmonstrate that the upregulation of TGF‑β1 in the bone tissue, blood and cerebrospinal fluid of patients with spinal cord injuries induced by thoracolumbar spine compression fractures is correlated with the downregulation of miR‑185. Furthermore, miR‑185 may target TGF‑β1, affecting its transcription and translation, indicating that it serves an important role in spinal cord injuries induced by thoracolumbar spine compression fractures.
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1 

Silverman SL: The clinical consequences of vertebral compression fracture. Bone. 13:(Suppl 2). S27–S31. 1992. View Article : Google Scholar : PubMed/NCBI

2 

Brown DB, Gilula LA, Sehgal M and Shimony JS: Treatment of chronic symptomatic vertebral compression fractures with percutaneous vertebroplasty. AJR Am J Roentgenol. 182:319–322. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Kwon BK, Tetzlaff W, Grauer JN, Beiner J and Vaccaro AR: Pathophysiology and pharmacologic treatment of acute spinal cord injury. Spine. 4:451–464. 2004. View Article : Google Scholar

4 

Hao D, He L and Yuan F: Late complications in patients with spinal cord injuries and related factors. Zhongguo Jizhu Jisui Zazhi. 15:267–270. 2005.

5 

Lee KZ and Chang YS: Recovery of the pulmonary chemoreflex and functional role of bronchopulmonary C-fibers following chronic cervical spinal cord injury. J Appl Physiol (1985). 117:1188–1198. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Murata K, Ito H, Yoshitomi H, Yamamoto K, Fukuda A, Yoshikawa J, Furu M, Ishikawa M, Shibuya H and Matsuda S: Inhibition of miR-92a enhances fracture healing via promoting angiogenesis in a model of stabilized fracture in young mice. J Bone Miner Res. 29:316–326. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Sheyn D, Kallai I, Tawackoli W, Yakubovich D Cohn, Oh A, Su S, Da X, Lavi A, Kimelman-Bleich N, Zilberman Y, et al: Gene-modified adult stem cells regenerate vertebral bone defect in a rat model. Mol Pharm. 8:1592–1601. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Hulth A: Current concepts of fracture healing. Clin Orthop Relat Res. 249:265–284. 1989.

9 

Tatsuyama K, Maezawa Y, Baba H, Imamura Y and Fukuda M: Expression of various growth factors for cell proliferation and cytodifferentiation during fracture repair of bone. Eur J Histochem. 44:269–278. 2000.PubMed/NCBI

10 

Kiefer R, Streit WJ, Toyka KV, Kreutzberg GW and Hartung HP: Transforming growth factor-beta 1: A lesion-associated cytokine of the nervous system. Int J Dev Neurosci. 13:331–339. 1995. View Article : Google Scholar : PubMed/NCBI

11 

Flanders KC, Ren RF and Lippa CF: Transforming growth factor-betas in neurodegenerative disease. Prog Neurobiol. 54:71–85. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Hiraizumi Y, Fujimaki E, Transfeldt EE, Kawahara N, Fiegel VD, Knighton D and Sung JH: The effect of the platelet derived wound healing formula and the nerve growth factor on the experimentally injured spinal cord. Spinal Cord. 34:394–402. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Jia L: Modern spinal surgery. 1st. People's Military Medical Press; Beijing: pp. 848–859. 2007

15 

Tian W: Practical Orthopedics (1st). People's Medical Publishing House. Beijing: 562–563. 2008.

16 

Wu T, Liu Y, Fan Z, Xu J, Jin L, Gao Z, Wu Z, Hu L, Wang J, Zhang C, et al: miR-21 Modulates the Immunoregulatory Function of Bone Marrow Mesenchymal Stem Cells Through the PTEN/Akt/TGF-β1 Pathway. Stem Cells. 33:3281–3290. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Canalis E, McCarthy T and Centrella M: Growth factors and the regulation of bone remodeling. J Clin Invest. 81:277–281. 1988. View Article : Google Scholar : PubMed/NCBI

18 

Pfeilschifter J, Diel I, Scheppach B, Bretz A, Krempien R, Erdmann J, Schmid G, Reske N, Bismar H, Seck T, et al: Concentration of transforming growth factor beta in human bone tissue: Relationship to age, menopause, bone turnover and bone volume. J Bone Miner Res. 13:716–730. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Joyce ME, Roberts AB, Sporn MB and Bolander ME: Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur. J Cell Biol. 110:2195–2207. 1990. View Article : Google Scholar : PubMed/NCBI

20 

Centrella M, McCarthy TL and Canalis E: Transforming growth factor beta is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone. J Biol Chem. 262:2869–2874. 1987.PubMed/NCBI

21 

Unsicker K and Krieglstein K: TGF-betas and their roles in the regulation of neuron survival. Adv Exp Med Biol. 513:353–374. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Tyor WR, Avgeropoulos N, Ohlandt G and Hogan EL: Treatment of spinal cord impact injury in the rat with transforming growth factor-beta. J Neurol Sci. 200:33–41. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Want T and Feng Z: Polypeptide growth factors and spinal cord injury. Health Science and Technology Publishing House in Xinjiang; Fitst. Urumqi: pp. 104–105. 2003

24 

Romão JE Junior, Haiashi AR, Vidonho AF Junior, Abensur H, Quintaes PS, Araújo MR, Noronha IL, Santos FR and Machado MM: Causes and prognosis of acute renal failure in elderly patients. Rev Assoc Med Bras (1992). 46:212–217. 2000.(In Portuguese). PubMed/NCBI

25 

Gudi V, Škuljec J, Yildiz Ö, Frichert K, Skripuletz T, Moharregh-Khiabani D, Voss E, Wissel K, Wolter S and Stangel M: Spatial and temporal profiles of growth factor expression during CNS demyelination reveal the dynamics of repair priming. PLoS One. 6:e226232011. View Article : Google Scholar : PubMed/NCBI

26 

Kim D, Schallert T, Liu Y, Browarak T, Nayeri N, Tessler A and Murray M Fischer: Transplantation of genetically modified fibroblasts expressing BDNF in adult rats with a subtotal hemisection improves specific motor and sensory functions. Neurorehabil Neural Repair. 15:141–150. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Zhao X, Mohan R, Özcan S and Tang X: MicroRNA-30d induces insulin transcription factor MafA and insulin production by targeting mitogen-activated protein 4 kinase 4 (MAP4K4) in pancreatic β-cells. J Biol Chem. 287:31155–31164. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Chen K and Rajewsky N: The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Genet. 8:93–1037. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Bai W, Chen Y, Yang J, Niu P, Tian L and Gao A: Aberrant miRNA profiles associated with chronic benzene poisoning. Exp Mol Pathol. 96:426–430. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Kim JO, Song DW, Kwon EJ, Hong SE, Song HK, Min CK and Kim DH: miR-185 Plays an Anti-Hypertrophic Role in the Heart via Multiple Targets in the Calcium-Signaling pathways. PLoS One. 10:e01225092015. View Article : Google Scholar : PubMed/NCBI

32 

Ma X, Shen D, Li H, Zhang Y, Lv X, Huang Q, Gao Y, Li X, Gu L, Xiu S, et al: MicroRNA-185 inhibits cell proliferation and induces cell apoptosis by targeting VEGFA directly in von Hippel-Lindau-inactivated clear cell renal cell carcinoma. Urol Oncol. 33:169.e1-112015. View Article : Google Scholar : PubMed/NCBI

33 

Bao L, Fu X, Si M, Wang Y, Ma R, Ren X and Lv H: MicroRNA-185 targets SOCS3 to inhibit beta-cell dysfunction in diabetes. PLoS One. 10:e01160672015. View Article : Google Scholar : PubMed/NCBI

34 

Fu P, Du F, Yao M, Lv K and Liu Y: MicroRNA-185 inhibits proliferation by targeting c-Met in human breast cancer cells. Exp Ther Med. 8:1879–1883. 2014.PubMed/NCBI

35 

Wang R, Tian S, Wang HB, Chu DP, Cao JL, Xia HF and Ma X: MiR-185 is involved in human breast carcinogenesis by targeting Vegfa. FEBS Lett. 588:4438–4447. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Chang CZ, Wu SC, Lin CL and Kwan AL: Curcumin, encapsulated in nano-sized PLGA, down-regulates nuclear factor κB (p65) and subarachnoid hemorrhage induced early brain injury in a rat model. Brain Res. 1608:215–224. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao P, Wang S, Zhou Y, Zheng H and Zhao G: MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1. Exp Ther Med 13: 1127-1132, 2017.
APA
Zhao, P., Wang, S., Zhou, Y., Zheng, H., & Zhao, G. (2017). MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1. Experimental and Therapeutic Medicine, 13, 1127-1132. https://doi.org/10.3892/etm.2017.4052
MLA
Zhao, P., Wang, S., Zhou, Y., Zheng, H., Zhao, G."MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1". Experimental and Therapeutic Medicine 13.3 (2017): 1127-1132.
Chicago
Zhao, P., Wang, S., Zhou, Y., Zheng, H., Zhao, G."MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1". Experimental and Therapeutic Medicine 13, no. 3 (2017): 1127-1132. https://doi.org/10.3892/etm.2017.4052
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao P, Wang S, Zhou Y, Zheng H and Zhao G: MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1. Exp Ther Med 13: 1127-1132, 2017.
APA
Zhao, P., Wang, S., Zhou, Y., Zheng, H., & Zhao, G. (2017). MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1. Experimental and Therapeutic Medicine, 13, 1127-1132. https://doi.org/10.3892/etm.2017.4052
MLA
Zhao, P., Wang, S., Zhou, Y., Zheng, H., Zhao, G."MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1". Experimental and Therapeutic Medicine 13.3 (2017): 1127-1132.
Chicago
Zhao, P., Wang, S., Zhou, Y., Zheng, H., Zhao, G."MicroRNA‑185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor‑β1". Experimental and Therapeutic Medicine 13, no. 3 (2017): 1127-1132. https://doi.org/10.3892/etm.2017.4052
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