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Article

Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds

  • Authors:
    • Fen Yang
    • Bing Shi
    • Ling Cao
  • View Affiliations / Copyright

    Affiliations: Department of Burns and Plastic Surgery, Bayannur City Hospital, Bayannur, Inner Mongolia 015000, P.R. China, Department of Plastic and Reconstructive Surgery, The 309th Hospital of PLA, Beijing 100091, P.R. China
  • Pages: 1081-1086
    |
    Published online on: January 18, 2017
       https://doi.org/10.3892/etm.2017.4057
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Abstract

The aim of the present study was to compare and observe the expression levels of vascular endothelial growth factor (VEGF) and miRNA‑17‑5p during the treatment of seawater‑immersed blast‑injury wounds (SIBIW) under different conditions of vacuum sealing drainage (VSD), and to identify the optimized range of VSD treatment and partially explain its mechanisms. The bilateral hips and scapulae of experimental pigs (weight, 25‑30 kg) were subjected to blast‑injury wounds, followed by the seawater immersion. The animals then underwent conventional dressing treatment under 120, 180 and 240 mmHg VSD. Visual observation, in addition to histological, immunohistochemical and molecular biological techniques were applied to compare and observe the extent of wound healing and expression levels of VEGF and miRNA‑17‑5p. The wound healing of the VSD treatment group was improved compared with the control group, with 120 mmHg negative pressure producing the most marked effect. miR‑17‑5p expression was detected in the SIBIW granulation tissues. There was significant difference between each VSD treatment group and control group at each time point (P<0.05). Thus, the present results show that miR‑17‑5p can be expressed in SIBIW granulation tissues, and this effect is most evident under 120 mmHg negative pressure, which may inform the optimized negative range for the treatment of SIBIW.
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View References

1 

Kirkman E and Watts S: Haemodynamic changes in trauma. Br J Anaesth. 113:266–275. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Ning J, Mo L, Zhao H, Lu K, Wang L, Lai X, Yang B, Zhao H, Sanders RD and Ma D: Transient regional hypothermia applied to a traumatic limb attenuates distant lung injury following biast limb trauma. Crit Care Med. 42:e68–e78. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Liu P, Liu JC, Lai XN, Peng XL, Wu GP, Zhang LC and Wang LL: Pathological study of rabbits' femoral arteries subjected to gunshot wounds combining with seawater immersion. Chin J Traumatol. 8:186–190. 2005.PubMed/NCBI

4 

Fleischmann W, Strecker W, Bombelli M and Kinzl L: Vacuum sealing as treatment of soft tissue damage in open fractures. Unfallchirurg. 96:488–492. 1993.PubMed/NCBI

5 

Witkowski W, Jawien A, Witkiewicz W and Zon B: Initial multi-centre observations upon the effect of a new topical negative pressure device upon patient and clinician experience and the treatment of wounds. Int Wound J. 6:167–174. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Scherer LA, Shiver S, Chang M, Meredith JW and Owings JT: The vacuum assisted closure device: A method of securing skin grafts and improving graft survival. Arch surg. 137:930–934; discussion 933–934. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Zhang C, Liu DR, Liang Z, Liu F, Lin H and Guo Z: Reapair of refractory wounds through grafting of artificial dermis and autologous epidermis aided by vacuum-assistde closure. Aesthetic Plast Surg. 38:727–732. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Li XY, Li JQ, Chen SZ, Li WZ and Li YJ: Establishment of animal model of blast injury in pig skin and soft tissue. Zhong Hua Shi Yan Wai Ke Za Zhi. 12:1551–1553. 2006.(in Chinese).

9 

Pazyar N, Yaghoobi R, Rafiee E, Mehrabian A and Feily A: Skin wound healing and phytomedicine: A review. Skin Pharmacol Physio. 2:303–310. 2014. View Article : Google Scholar

10 

Cao L, Peng MM, Sun JJ, Yu XC and Shi B: Application of vacuum-assisted closure in seawater-immersed wound treatment under different negative pressures. Genet Mol Res. 14:6146–6155. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Shi B, Sun J, Cao Y, Yang F, Wu Y, Liang X and Li L: Application of vacuum sealing drainage to the treatment of seawater-immersed blast-injury wounds. Int Wound J. May 8–2015.(Epub ahead of print).

12 

Popov VL, Kadochnikov DS and Minaeva PV: The role of the biological damaging factor in the explosive injury. Sud Med Ekspert. 58:20–23. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Diaz JH: Skin and soft tissue infections following marine injuries and exposures in travelers. J Travel Med. 21:207–213. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Diaz JH and Lopez FA: Skin, soft tissue and systemic bacterial infections following aquatic injuries and exposures. Am J Med Sci. 349:269–275. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Wolvos TA: Negative pressure wound therapy with instillation: The current state of the art. Surg Technol Int. 24:53–62. 2013.

16 

Frick A, Wallmichrath J, Frick G, Giunta RE and Engelhardt TO: Difficult wounds & vacuum assisted closure (VAC). MMW Fortschr Med. 155:63–64. 2013.(In German). View Article : Google Scholar : PubMed/NCBI

17 

Altintas B, Biber R and Brem MH: The accelerating effect of negative pressure wound therapy with Prevena™ on the healing of a closed wound with persistent serous secretion. Int Wound J. 2014.(Epub ahead of print). PubMed/NCBI

18 

Zhang WH, Wu Q, Ma J and Wang JH: Effects of vacuum drainage combined with heparin irrigation for treatment of scald burns with seawaterimmersion in rabbits. Nan Fang Yi Ke Da Xue Xue Bao. 35:1481–1486. 2015.(in Chinese). PubMed/NCBI

19 

Ingber DE: Tensegrity I. Cell structure and hierarchical systems biology. J Cell Sci. 116:1157–1173. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Huljev D: Negative pressure therapy-supportive method in chronic wound treatment. Acta Med Croatica. 67:(Suppl 1). S89–S94. 2013.(In Croatian).

21 

Esteban MP Borrero, Begines R Begines, Rodríguez Llamas S and Díaz Campos T: Managing complications in severe traumatic injury with VAC therapy with instillation. Rev Enferm. 36:42–47. 2013.(In Spanish).

22 

Malmsjö M, Lindstedt S, Ingemansson R and Gustafsson L: Use of bacteria- and fungus-binding mesh in negative pressure wound therapy provides significant granulation tissue without tissue ingrowth. Eplasty. 14:e32014.PubMed/NCBI

23 

Harish V and Maitz PK: Uninterrupted continuous negative pressure wound therapy is safe and can facilitate engraftment of dermal regeneration templates. J Plast Reconstr Aesthet Surg. 67:1011–1013. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Pitt KA and Stanley BJ: Negative pressure wound therapy: Experience in 45 dogs. Vet Surg. 43:380–387. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Atay T, Burc H, Baykal YB and Kirdemir V: Results of vacuum assisted wound closure application. Indian J Surg. 75:302–305. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Roy S and Sen CK: miRNA in wound inflammation and angiogenesis. Microcirculation. 19:224–232. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Hildebrand J, Rütze M, Walz N, Gallinat S, Wenck H, Deppert W, Grundhoff A and Knott A: A comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivo. Invest Dermatol. 131:20–29. 2011. View Article : Google Scholar

28 

Zhang L, Stokes N, Polak L and Fuchs E: Specific microRNAs are preferentially expressed by skin stem cells to balance self-renewal and early lineage commitment. Cell Stem Cell. 8:294–308. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Chen Y and Gorski DH: Regulation of angiogenesis through a microRNA (miR-130a) that down-regulates antiangiogenic homeobox genes GAX and HOXA5. Blood. 111:1217–1226. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Suárez Y, Fernández-Hernando C, Yu J, Gerber SA, Harrison KD, Pober JS, Iruela-Arispe ML, Merkenschlager M and Sessa WC: Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proc Natl Acad Sci USA. 105:14082–14087. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Otsuka M, Zheng M, Hayashi M, Lee JD, Yoshino O, Lin S and Han J: Impaired microRNA processing causes corpus lustrum insufficiency and infertility in mice. J Clin Invest. 118:1944–1954. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Ramón LA, Braza-Boïls A, Gilabert J, Chirivella M, España F, Estellés A and Gilabert-Estellés J: microRNAs related to angiogenesis is deregulated in endometriosis endometrial cancer. Hum Reprod. 27:3036–3045. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Bonauer A, Carmona G, Iwasaki M, Mione M, Koyanagi M, Fischer A, Burchfield J, Fox H, Doebele C, Ohtani K, et al: MicroRNA-92a controls angiogenesis and functional recovery of ischemic tissues in mice. Science. 324:1710–1713. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang F, Shi B and Cao L: Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds. Exp Ther Med 13: 1081-1086, 2017.
APA
Yang, F., Shi, B., & Cao, L. (2017). Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds. Experimental and Therapeutic Medicine, 13, 1081-1086. https://doi.org/10.3892/etm.2017.4057
MLA
Yang, F., Shi, B., Cao, L."Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds". Experimental and Therapeutic Medicine 13.3 (2017): 1081-1086.
Chicago
Yang, F., Shi, B., Cao, L."Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds". Experimental and Therapeutic Medicine 13, no. 3 (2017): 1081-1086. https://doi.org/10.3892/etm.2017.4057
Copy and paste a formatted citation
x
Spandidos Publications style
Yang F, Shi B and Cao L: Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds. Exp Ther Med 13: 1081-1086, 2017.
APA
Yang, F., Shi, B., & Cao, L. (2017). Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds. Experimental and Therapeutic Medicine, 13, 1081-1086. https://doi.org/10.3892/etm.2017.4057
MLA
Yang, F., Shi, B., Cao, L."Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds". Experimental and Therapeutic Medicine 13.3 (2017): 1081-1086.
Chicago
Yang, F., Shi, B., Cao, L."Effect of vacuum sealing drainage on the expression of VEGF and miRNA‑17‑5p in seawater‑immersed blast‑injury wounds". Experimental and Therapeutic Medicine 13, no. 3 (2017): 1081-1086. https://doi.org/10.3892/etm.2017.4057
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