Open Access

Research on the effect of ginseng polysaccharide on apoptosis and cell cycle of human leukemia cell line K562 and its molecular mechanisms

  • Authors:
    • Wei Xiong
    • Jing Li
    • Rong Jiang
    • Danyang Li
    • Zehong Liu
    • Dilong Chen
  • View Affiliations

  • Published online on: January 24, 2017     https://doi.org/10.3892/etm.2017.4087
  • Pages: 924-934
  • Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ginseng polysaccharide (GPS), a polymer of glucose and the primary constituent extracted from panax ginseng, has been documented to exert various pharmacological properties, including anti‑tumor properties. To provide further insights into the anti‑tumor functions of GPS, the present study was designed to investigate the effect of GPS on apoptosis and the cell cycle of human leukemia cell line K562 cells, and its underlying mechanisms. The results demonstrated that GPS could inhibit K562 cell proliferation and induce apoptosis in vitro in a concentration‑ and time‑dependent manner. The transcription of P38 and c‑Jun NH2‑terminal kinase (JNK) mRNA were significantly augmented, while the transcription of extracellular signal‑regulated kinase (ERK) mRNA were significantly reduced following treatment with GPS compared with the control group (all P<0.05). In addition, GPS treatment markedly suppressed the expression of phosphorylated (p)‑ERK, nuclear factor (NF)‑κB p65 and cyclin D1, and increased the synthesis of p‑P38 and p‑JNK protein expression, as evidenced by immunofluorescence and western blotting analyses. In conclusion, the results indicate that the GPS‑mediated MAPK/NF‑κB/cyclin D1 signaling pathway serves a crucial role in cell cycle arrest and apoptosis of K562 cells.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xiong W, Li J, Jiang R, Li D, Liu Z and Chen D: Research on the effect of ginseng polysaccharide on apoptosis and cell cycle of human leukemia cell line K562 and its molecular mechanisms. Exp Ther Med 13: 924-934, 2017
APA
Xiong, W., Li, J., Jiang, R., Li, D., Liu, Z., & Chen, D. (2017). Research on the effect of ginseng polysaccharide on apoptosis and cell cycle of human leukemia cell line K562 and its molecular mechanisms. Experimental and Therapeutic Medicine, 13, 924-934. https://doi.org/10.3892/etm.2017.4087
MLA
Xiong, W., Li, J., Jiang, R., Li, D., Liu, Z., Chen, D."Research on the effect of ginseng polysaccharide on apoptosis and cell cycle of human leukemia cell line K562 and its molecular mechanisms". Experimental and Therapeutic Medicine 13.3 (2017): 924-934.
Chicago
Xiong, W., Li, J., Jiang, R., Li, D., Liu, Z., Chen, D."Research on the effect of ginseng polysaccharide on apoptosis and cell cycle of human leukemia cell line K562 and its molecular mechanisms". Experimental and Therapeutic Medicine 13, no. 3 (2017): 924-934. https://doi.org/10.3892/etm.2017.4087