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Article Open Access

Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells

  • Authors:
    • Xiao-Fei Yang
    • Tao Chen
    • Li-Wei Ren
    • Lu Yang
    • Hui Qi
    • Fu-Rong Li
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Stem Cell and Cellular Therapy, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen, Guangdong 518020, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1456-1464
    |
    Published online on: February 2, 2017
       https://doi.org/10.3892/etm.2017.4096
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Abstract

Mesenchymal stem cells (MSCs) have been considered as hypo-immunogenic and immunosuppressive. However, a thorough understanding of the immunological properties after MSC differentiation in vitro and in vivo has not been reached. We asked whether it would be immunogenic after differentiation or influenced by the immune microenvironment after transplantation. In different disease models, the immunological changes of MSCs after differentiation greatly varied, with contradicting results. In order to clarify this, we used a modified four-step induction method to induce human umbilical cord MSCs (hUCMSCs) to differentiate into insulin‑producing cells (IPCs), and investigate the immunological changes after differentiation and immune reactions after transplantation into diabetic mice. We found that the induced IPCs are hypo-immunogenic, lacking HLA-DR, CD40 and CD80 expression. Of note, we observed immune cell infiltration to peritoneal cavity and left kidney capsule after local transplantation of induced IPCs. This indicated that hUCMSC-derived IPCs maintained hypo‑immunogenic in vitro, but became immunogenic after transplanting to the host, possibly due to the changes of immune microenvironment and thereafter immunological enhancement and immune cell infiltration.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Chen T, Ren L, Yang L, Qi H and Li F: Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells. Exp Ther Med 13: 1456-1464, 2017.
APA
Yang, X., Chen, T., Ren, L., Yang, L., Qi, H., & Li, F. (2017). Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells. Experimental and Therapeutic Medicine, 13, 1456-1464. https://doi.org/10.3892/etm.2017.4096
MLA
Yang, X., Chen, T., Ren, L., Yang, L., Qi, H., Li, F."Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells". Experimental and Therapeutic Medicine 13.4 (2017): 1456-1464.
Chicago
Yang, X., Chen, T., Ren, L., Yang, L., Qi, H., Li, F."Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1456-1464. https://doi.org/10.3892/etm.2017.4096
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Chen T, Ren L, Yang L, Qi H and Li F: Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells. Exp Ther Med 13: 1456-1464, 2017.
APA
Yang, X., Chen, T., Ren, L., Yang, L., Qi, H., & Li, F. (2017). Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells. Experimental and Therapeutic Medicine, 13, 1456-1464. https://doi.org/10.3892/etm.2017.4096
MLA
Yang, X., Chen, T., Ren, L., Yang, L., Qi, H., Li, F."Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells". Experimental and Therapeutic Medicine 13.4 (2017): 1456-1464.
Chicago
Yang, X., Chen, T., Ren, L., Yang, L., Qi, H., Li, F."Immunogenicity of insulin-producing cells derived from human umbilical cord mesenchymal stem cells". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1456-1464. https://doi.org/10.3892/etm.2017.4096
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