Open Access

Calcitonin gene-related peptide protects type II alveolar epithelial cells from hyperoxia-induced DNA damage and cell death

  • Authors:
    • Hongmin Fu
    • Tiesong Zhang
    • Rongwei Huang
    • Zhen Yang
    • Chunming Liu
    • Ming Li
    • Fang Fang
    • Feng Xu
  • View Affiliations

  • Published online on: February 16, 2017     https://doi.org/10.3892/etm.2017.4132
  • Pages: 1279-1284
  • Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hyperoxia therapy for acute lung injury (ALI) may unexpectedly lead to reactive oxygen species (ROS) production and cause additional ALI. Calcitonin gene‑related peptide (CGRP) is a 37 amino acid neuropeptide that regulates inflammasome activation. However, the role of CGRP in DNA damage during hyperoxia is unclear. Therefore, the aim of the present study was to investigate the effects of CGRP on DNA damage and the cell death of alveolar epithelial type II cells (AEC II) exposed to 60% oxygen. AEC II were isolated from 19‑20 gestational day fetal rat lungs and were exposed to air or to 60% oxygen during treatment with CGRP or the specific CGRP receptor antagonist CGRP8‑37. The cells were evaluated using immunofluorescence to examine surfactant protein‑C and ROS levels were measured by probing with 2',7'-dichlorofluorescin diacetate. The apoptosis rate and cell cycle of AEC II were analyzed by flow cytometry, and apoptosis was determined by western blotting analysis of activated caspase 3. The DNA damage was confirmed with immunofluorescence of H2AX via high‑content analysis. The ROS levels, apoptotic cell number and the expression of γH2AX were markedly increased in the hyperoxia group compared with those in the air group. Concordantly, ROS levels, apoptotic cell number and the expression of γH2AX were significantly lower with a significant arrest of S and G2/M phases in the CGRP/O2 group than in the hyperoxia or CGRP8‑37/O2 groups. CGRP was concluded to protect lung epithelium cells against hyperoxic insult, and upregulation of CGRP may be a possible novel therapeutic target to treat hyperoxic lung injury.
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April-2017
Volume 13 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Fu H, Zhang T, Huang R, Yang Z, Liu C, Li M, Fang F and Xu F: Calcitonin gene-related peptide protects type II alveolar epithelial cells from hyperoxia-induced DNA damage and cell death. Exp Ther Med 13: 1279-1284, 2017
APA
Fu, H., Zhang, T., Huang, R., Yang, Z., Liu, C., Li, M. ... Xu, F. (2017). Calcitonin gene-related peptide protects type II alveolar epithelial cells from hyperoxia-induced DNA damage and cell death. Experimental and Therapeutic Medicine, 13, 1279-1284. https://doi.org/10.3892/etm.2017.4132
MLA
Fu, H., Zhang, T., Huang, R., Yang, Z., Liu, C., Li, M., Fang, F., Xu, F."Calcitonin gene-related peptide protects type II alveolar epithelial cells from hyperoxia-induced DNA damage and cell death". Experimental and Therapeutic Medicine 13.4 (2017): 1279-1284.
Chicago
Fu, H., Zhang, T., Huang, R., Yang, Z., Liu, C., Li, M., Fang, F., Xu, F."Calcitonin gene-related peptide protects type II alveolar epithelial cells from hyperoxia-induced DNA damage and cell death". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1279-1284. https://doi.org/10.3892/etm.2017.4132