Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model

Ezirganli,Şeref; Kazancioğlu,Hakki   Oğuz; Acar,Ahmet  Hüseyin; Özdemir,Hakan; Kuzu,Emre; İnan,Deniz  Şahin

doi:10.3892/etm.2017.4166

Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model

Authors:
- Şeref Ezirganli
- Hakki Oğuz Kazancioğlu
- Ahmet Hüseyin Acar
- Hakan Özdemir
- Emre Kuzu
- Deniz Şahin İnan
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Affiliations: Program of Dental Assisting, Vocational School of Health Services, Institute of Health Science, Nişantası University, Bayrampaşa, 34030 Istanbul, Turkey, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, Fatih, 34093 Istanbul, Turkey, Department of Periodontology, Faculty of Dentistry, Osmangazi University, 26480 Eskişehir, Turkey, Dental Health Centre of Sivas, Rebublic of Turkey Ministry of Health, 58020 Sivas, Turkey, Department of Histology and Embryology, Faculty of Medicine, Cumhuriyet University, 58140 Sivas, Turkey
Published online on: March 1, 2017 https://doi.org/10.3892/etm.2017.4166
Pages: 1827-1831
Copyright: © Ezirganli et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone defects resulting from oncological surgical resections, congenital facial anomalies, trauma or infection represent a significant and common clinical problem. The present study aimed to evaluate the effects of a commercially-available medicinal plant extract product, Ankaferd BloodStopper (ABS), on bone healing. The present study was performed on 24 female ovariectomized (OVX) rats. A defect on each rat calvarium was created using a trephine burr prior to the rats being divided into two groups. Defects were grafted with a gelatin sponge soaked with normal saline (control group) or plant extract (experimental group). Half of the animals were sacrificed after 2 weeks and the others after 4 weeks. In the control group, the defects were not filled with regenerated bone. By contrast, in the experimental group, all defect areas had an increased amount of regenerated bone and connective tissue. Osteoblastic activity appeared to be greater in the experimental group however, osteoclastic activity was observed to be higher in the control group. At 2 and 4 weeks, there was a significant difference in the amount of newly regenerated bone observed in the experimental group compared with the control group (P<0.05). Therefore, the results of the present study indicated that local ABS application had a positive effect on bone healing in the OVX rat model.

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