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Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling

Expression of Concern in: /10.3892/etm.2025.12972
  • Authors:
    • Anca Hermenean
    • Teodora Mariasiu
    • Inmaculada Navarro‑González
    • Josefina Vegara‑Meseguer
    • Eftimie Miuțescu
    • Sandipan Chakraborty
    • Horacio Pérez‑Sánchez
  • View Affiliations / Copyright

    Affiliations: Department of Histology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310414 Arad, Romania, Department of Food Science and Nutrition, Faculty of Veterinary Science, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain, Bioinformatics and High Performance Computing Research Group, Saint Anthony Catholic University of Murcia, 30107 Murcia, Spain, Department of Gastroenterology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310414 Arad, Romania, Department of Microbiology, University of Calcutta, Kolkata, West Bengal 700019, India
    Copyright: © Hermenean et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1671-1680
    |
    Published online on: March 2, 2017
       https://doi.org/10.3892/etm.2017.4181
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Abstract

Chrysin (5,7-dihydroxyflavone) is a naturally occurring flavonoid present at high levels in honey, propolis and numerous plant extracts. Chrysin is known to have hepatoprotective activity, however, the mechanisms by which it exerts this effect remain unclear. In the present study, the effects of chrysin in carbon tetrachloride (CCl4)‑induced acute liver damage were investigated and the results used to infer a possible mechanism behind chrysin's hepatoprotective activity. Prior to an intraperitoneal injection of CCl4 (1 ml/kg) to induce acute liver damage, chrysin (50 mg/kg) was administered orally to mice for 7 days. The positive control group was given 50 mg/kg standardized silymarin, a well-studied hepatoprotective flavonoid. Twenty‑four h following CCl4 administration, an increase in the activity levels of serum aspartate-amino-transferase and alanine‑amino‑transferase was found. This was accompanied by extended centrilobular necrosis, steatosis and an altered hepatocyte ultrastructure. In addition, CCl4‑induced acute hepatotoxicity was associated with an increase in hepatic tumor necrosis factor‑α (TNF‑α) and α‑smooth muscle actin (α-SMA) protein expression, which was significantly decreased in the livers of mice pre‑treated with chrysin (P<0.001), similar to the results of the silymarin pre‑treated group (P<0.001). Treatment with chrysin prior to CCl4 exposure significantly reduced the activity of enzymes used as biochemical markers of poor liver function compared with the group which did not receive pre‑treatment (P<0.001). In addition, the results of histopathological and electron microscopy liver examination showed chrysin pre‑treatment reduced the effects of CCl4 treatment. Molecular modeling results demonstrated that the hepatoprotective activity of chrysin is mediated through TNF‑α, as it reduces soluble TNF‑α generation via blocking TNF‑α‑converting enzyme activity. In conclusion, the results of the present study suggest that inflammatory pathways are activated in CCl4‑induced acute liver damage, which are ameliorated by chrysin pre-treatment. This indicates that chrysin is a potent hepatoprotective agent, similarly to silymarin at the same dose, which has the potential to be a viable alternative to conventional hepatoprotective treatments.
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Copy and paste a formatted citation
Spandidos Publications style
Hermenean A, Mariasiu T, Navarro‑González I, Vegara‑Meseguer J, Miuțescu E, Chakraborty S and Pérez‑Sánchez H: Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972. Exp Ther Med 13: 1671-1680, 2017.
APA
Hermenean, A., Mariasiu, T., Navarro‑González, I., Vegara‑Meseguer, J., Miuțescu, E., Chakraborty, S., & Pérez‑Sánchez, H. (2017). Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972. Experimental and Therapeutic Medicine, 13, 1671-1680. https://doi.org/10.3892/etm.2017.4181
MLA
Hermenean, A., Mariasiu, T., Navarro‑González, I., Vegara‑Meseguer, J., Miuțescu, E., Chakraborty, S., Pérez‑Sánchez, H."Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972". Experimental and Therapeutic Medicine 13.5 (2017): 1671-1680.
Chicago
Hermenean, A., Mariasiu, T., Navarro‑González, I., Vegara‑Meseguer, J., Miuțescu, E., Chakraborty, S., Pérez‑Sánchez, H."Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972". Experimental and Therapeutic Medicine 13, no. 5 (2017): 1671-1680. https://doi.org/10.3892/etm.2017.4181
Copy and paste a formatted citation
x
Spandidos Publications style
Hermenean A, Mariasiu T, Navarro‑González I, Vegara‑Meseguer J, Miuțescu E, Chakraborty S and Pérez‑Sánchez H: Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972. Exp Ther Med 13: 1671-1680, 2017.
APA
Hermenean, A., Mariasiu, T., Navarro‑González, I., Vegara‑Meseguer, J., Miuțescu, E., Chakraborty, S., & Pérez‑Sánchez, H. (2017). Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972. Experimental and Therapeutic Medicine, 13, 1671-1680. https://doi.org/10.3892/etm.2017.4181
MLA
Hermenean, A., Mariasiu, T., Navarro‑González, I., Vegara‑Meseguer, J., Miuțescu, E., Chakraborty, S., Pérez‑Sánchez, H."Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972". Experimental and Therapeutic Medicine 13.5 (2017): 1671-1680.
Chicago
Hermenean, A., Mariasiu, T., Navarro‑González, I., Vegara‑Meseguer, J., Miuțescu, E., Chakraborty, S., Pérez‑Sánchez, H."Hepatoprotective activity of chrysin is mediated through TNF-α in chemically-induced acute liver damage: An in vivo study and molecular modeling Expression of Concern in /10.3892/etm.2025.12972". Experimental and Therapeutic Medicine 13, no. 5 (2017): 1671-1680. https://doi.org/10.3892/etm.2017.4181
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