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Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury

  • Authors:
    • Jun Li
    • Weichun Guo
    • Min Xiong
    • Shuangjie Zhang
    • Heng Han
    • Jie Chen
    • Dan Mao
    • Hualong Yu
    • Yun Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Orthopedics, Dongfeng General Hospital of Hubei University of Medicine, Shiyan, Hubei 442008, P.R. China, Department of Urology, Taihe Hospital of Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1806-1812
    |
    Published online on: March 6, 2017
       https://doi.org/10.3892/etm.2017.4182
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Abstract

Despite the successes of bone marrow mesenchymal stem cell (BMSC) transplantation for the treatment of spinal cord injuries, only a small fraction of grafted cells migrate to the target areas. Therefore, there remains a need for more efficient strategies of BMSC delivery. The present study was designed to explore this. Rat models of spinal cord injury (SCI) were established and exposed to phosphate buffered saline (control), BMSCs or BMSCs + erythropoietin (EPO). Basso, Beattie and Bresnahan (BBB) locomotor scale and grid walk tests were then utilized to estimate neurological rehabilitation. Additionally, the following assays were performed: Immunofluorescence localization of BMSCs to the site of SCI; the transwell migration assay to detect in vitro cellular migration; the terminal deoxynucleotidyl transferase dUTP nick end labeling assay to determine the apoptotic index of the lesion; and western blotting analysis to evaluate the expression of vascular endothelial growth factor (VEGF) and brain derived neurotrophic factor (BDNF) at the site of SCI. The BBB scores of the BMSC + EPO treated group were significantly increased compared with the BMSC treatment group (P<0.05). For example, BMSC + EPO treated rats had a significantly decreased number of hind limb slips compared with the BMSC treatment group (P<0.05). Furthermore, EPO significantly increased the migration capacity of BMSCs compared with the control group (P<0.001). In addition, the apoptotic index of the BMSC + EPO group was significantly decreased compared with the BMSC group (P<0.05). Green fluorescent protein‑labeled BMSCs were detected at the site of SCI in the BMSC and BMSCs + EPO groups, with the signal being notably stronger in the latter. Moreover, the expression of VEGF and BDNF in the BMSCs + EPO group was significantly increased compared with the BMSC group (P<0.05). In conclusion, the results of the present study indicate that EPO can facilitate the recruitment of BMSCs to sites of SCI, increase expression of BDNF and VEGF, and accelerate recovery of neurological function following SCI.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Guo W, Xiong M, Zhang S, Han H, Chen J, Mao D, Yu H and Zeng Y: Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury. Exp Ther Med 13: 1806-1812, 2017.
APA
Li, J., Guo, W., Xiong, M., Zhang, S., Han, H., Chen, J. ... Zeng, Y. (2017). Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury. Experimental and Therapeutic Medicine, 13, 1806-1812. https://doi.org/10.3892/etm.2017.4182
MLA
Li, J., Guo, W., Xiong, M., Zhang, S., Han, H., Chen, J., Mao, D., Yu, H., Zeng, Y."Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury". Experimental and Therapeutic Medicine 13.5 (2017): 1806-1812.
Chicago
Li, J., Guo, W., Xiong, M., Zhang, S., Han, H., Chen, J., Mao, D., Yu, H., Zeng, Y."Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury". Experimental and Therapeutic Medicine 13, no. 5 (2017): 1806-1812. https://doi.org/10.3892/etm.2017.4182
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Guo W, Xiong M, Zhang S, Han H, Chen J, Mao D, Yu H and Zeng Y: Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury. Exp Ther Med 13: 1806-1812, 2017.
APA
Li, J., Guo, W., Xiong, M., Zhang, S., Han, H., Chen, J. ... Zeng, Y. (2017). Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury. Experimental and Therapeutic Medicine, 13, 1806-1812. https://doi.org/10.3892/etm.2017.4182
MLA
Li, J., Guo, W., Xiong, M., Zhang, S., Han, H., Chen, J., Mao, D., Yu, H., Zeng, Y."Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury". Experimental and Therapeutic Medicine 13.5 (2017): 1806-1812.
Chicago
Li, J., Guo, W., Xiong, M., Zhang, S., Han, H., Chen, J., Mao, D., Yu, H., Zeng, Y."Erythropoietin facilitates the recruitment of bone marrow mesenchymal stem cells to sites of spinal cord injury". Experimental and Therapeutic Medicine 13, no. 5 (2017): 1806-1812. https://doi.org/10.3892/etm.2017.4182
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