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Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors

  • Authors:
    • Ziwei Yu
    • Yongzhi Men
    • Pin Dong
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2029-2035
    |
    Published online on: March 6, 2017
       https://doi.org/10.3892/etm.2017.4183
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Abstract

The present study investigated whether co-culturing Schwann cells (SCs) with neural stem cells (NSCs) improves viability, direction of differentiation and secretion of brain‑derived neurotrophic factor (BDNF) and glial cell‑derived neurotrophic factor (GDNF) in NSCs. The three groups assessed were as follows: SCs, NSCs, and a co‑culture of SCs and NSCs. Cellular morphological changes were observed under an inverted phase contrast microscope and quantified. Cells were identified by immunofluorescence staining: S100 for SCs, Nestin for NSCs, microtubule associated protein (Map) 2 and NeuN for neurons and glial fibrillary acidic protein for astrocytes. Cell viability was evaluated by MTT assay. Secretion of BDNF and GDNF was quantified; mRNA expression was quantified by reverse transcription‑quantitative polymerase chain reaction. The majority of NSCs in the co‑cultured group differentiated into neurons. The cell survival rate of the co‑culture group was significantly higher than the other groups on days 3, 5 and 10 (P<0.01). The secretion of BDNF in the co‑culture group was significantly higher than NSCs on days 3, 5 and 7 (P<0.05), while the amount of GDNF in co‑culture was significantly higher than both NSCs and SCs on day 1 (P<0.05). BDNF and GDNF gene expression in the co‑culture group was significantly higher than SCs (P<0.01). Gene expression of Map2 in co‑culture group was also significantly higher than both NSC and SC groups (P<0.01). Therefore, co‑cultured SCs and NSCs promote differentiation of NSCs into neurons and secrete higher levels of neurotropic factors including BDNF and GDNF.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Z, Men Y and Dong P: Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors. Exp Ther Med 13: 2029-2035, 2017.
APA
Yu, Z., Men, Y., & Dong, P. (2017). Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors. Experimental and Therapeutic Medicine, 13, 2029-2035. https://doi.org/10.3892/etm.2017.4183
MLA
Yu, Z., Men, Y., Dong, P."Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors". Experimental and Therapeutic Medicine 13.5 (2017): 2029-2035.
Chicago
Yu, Z., Men, Y., Dong, P."Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2029-2035. https://doi.org/10.3892/etm.2017.4183
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Z, Men Y and Dong P: Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors. Exp Ther Med 13: 2029-2035, 2017.
APA
Yu, Z., Men, Y., & Dong, P. (2017). Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors. Experimental and Therapeutic Medicine, 13, 2029-2035. https://doi.org/10.3892/etm.2017.4183
MLA
Yu, Z., Men, Y., Dong, P."Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors". Experimental and Therapeutic Medicine 13.5 (2017): 2029-2035.
Chicago
Yu, Z., Men, Y., Dong, P."Schwann cells promote the capability of neural stem cells to differentiate into neurons and secret neurotrophic factors". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2029-2035. https://doi.org/10.3892/etm.2017.4183
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