Open Access

Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet‑rich fibrin granules for bone regeneration in a rabbit calvarial critical‑size defect model

  • Authors:
    • Xi Wang
    • Guanghui Li
    • Jia Guo
    • Lei Yang
    • Yiming Liu
    • Qiang Sun
    • Rui Li
    • Weiwei Yu
  • View Affiliations

  • Published online on: March 8, 2017     https://doi.org/10.3892/etm.2017.4199
  • Pages: 1891-1899
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The reconstruction of large bone defects remains a major clinical challenge, and tissue engineering is a promising technique for resolving this problem. Many attempts have been made to optimize bone tissue engineering protocols. The aim of the present study was to develop a process incorporating mesenchymal stem cell (MSC) sheets with nanoscale hydroxyapatite (nano‑HA) and autologous platelet‑rich fibrin (PRF) granules for enhanced bone formation within a critical‑sized rabbit cranial defect. MSC sheets and PRF were prepared prior to in vivo experiments. The osteogenic differentiation ability of MSCs and the ultrastructure of PRF were also studied. A total of 15 New Zealand white rabbits were used in the current study and critical‑size defects (CSDs) were surgically introduced in the cranium (diameter, 15 mm). The surgical defects were treated with MSC/PRF composites, MSC composites or left empty. Animals were euthanized at week 8 post‑surgery. Iconography, histological and histomorphometric analysis were performed to assess de novo bone formation. The percentage of new bone in the MSC/PRF group (35.7±5.1%) was significantly higher than that in the MSC (18.3±3.2%; P<0.05) and empty defect groups (4.7±1.5%; P<0.05). The results of the present study suggest that combined application of an MSC sheet with nano‑HA and granular PRF enhances bone regeneration in a rabbit calvarial CSD model, and provides a novel insight into bone tissue regeneration for large bone defects.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang X, Li G, Guo J, Yang L, Liu Y, Sun Q, Li R and Yu W: Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet‑rich fibrin granules for bone regeneration in a rabbit calvarial critical‑size defect model. Exp Ther Med 13: 1891-1899, 2017
APA
Wang, X., Li, G., Guo, J., Yang, L., Liu, Y., Sun, Q. ... Yu, W. (2017). Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet‑rich fibrin granules for bone regeneration in a rabbit calvarial critical‑size defect model. Experimental and Therapeutic Medicine, 13, 1891-1899. https://doi.org/10.3892/etm.2017.4199
MLA
Wang, X., Li, G., Guo, J., Yang, L., Liu, Y., Sun, Q., Li, R., Yu, W."Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet‑rich fibrin granules for bone regeneration in a rabbit calvarial critical‑size defect model". Experimental and Therapeutic Medicine 13.5 (2017): 1891-1899.
Chicago
Wang, X., Li, G., Guo, J., Yang, L., Liu, Y., Sun, Q., Li, R., Yu, W."Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet‑rich fibrin granules for bone regeneration in a rabbit calvarial critical‑size defect model". Experimental and Therapeutic Medicine 13, no. 5 (2017): 1891-1899. https://doi.org/10.3892/etm.2017.4199