Effects of glucocorticoid on the expression and regulation of aquaporin 5 in the paranasal sinus of rats with chronic rhinosinusitis

Yu,Chen‑Jie; Cui,Xin‑Yan; Lu,Ling; Yang,Jun; Chen,Bin; Zhu,Cheng‑Wen; Gao,Xia

doi:10.3892/etm.2017.4215

Effects of glucocorticoid on the expression and regulation of aquaporin 5 in the paranasal sinus of rats with chronic rhinosinusitis

Authors:
- Chen‑Jie Yu
- Xin‑Yan Cui
- Ling Lu
- Jun Yang
- Bin Chen
- Cheng‑Wen Zhu
- Xia Gao
View Affiliations

Affiliations: Department of Otolaryngology, Drum Tower Clinical Medical School, Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, Department of Otolaryngology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Otolaryngology, Nanjing Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210008, P.R. China, Department of Pathology, Nanjing Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210008, P.R. China, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, P.R. China
Published online on: March 9, 2017 https://doi.org/10.3892/etm.2017.4215
Pages: 1753-1756
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Aquaporins (AQPs) are water-specific membrane channel proteins that regulate water homeostasis for cells and organisms. AQP5 serves an important role in the maintenance of mucosal water homeostasis, and potentially contributes to mucosal edema and inflammation formation in chronic rhinosinusitis (CRS). The aim of the present study was to explore the expression pattern of AQP5 and the effect of glucocorticoids on AQP5 expression in rats with CRS. The rats were randomly divided into three equal groups, as follows: CRS, dexamethasone (dexa) treatment and control groups. A polyvinyl acetal material containing Staphylococcus aureus was inserted into the left nasal cavity of each rat from the CRS and dexa groups. On the 90th post‑operative day, the dexa group received dexamethasone (2 mg/kg/day) via intraperitoneal injection for 7 days. The controls did not receive any treatment. The expression of AQP5 in the sinonasal mucosa was determined using immunohistochemistry and quantitative PCR. The immunoreactivities of AQP5 were primarily noted in the epithelial lining and glandular cells, the vascular endothelium and in the goblet cells in the sinonasal mucosa. The AQP5 mRNA expression level was significantly higher in the dexa group than in the control and CRS groups (P=0.006 and P=0.014, respectively). However, no significant difference was indicated between the CRS and control groups (P=0.760). In conclusion, the current study suggests that glucocorticoids induce AQP5 expression in the sinonasal mucosa of CRS rats, which highlights AQP5 as a potential target in the diagnosis and treatment of CRS.

View Figures

View References

1	Fokkens WJ, Lund VJ, Mullol J, Bachert C, Alobid I, Baroody F, Cohen N, Cervin A, Douglas R, Gevaert P, et al: EPOS 2012: European position paper on rhinosinusitis and nasal polyps 2012. A summary for otorhinolaryngologists. Rhinology. 50:1–12. 2012.PubMed/NCBI
2	Hastan D, Fokkens WJ, Bachert C, Newson RB, Bislimovska J, Bockelbrink A, Bousquet PJ, Brozek G, Bruno A, Dahlén SE, et al: Chronic rhinosinusitis in Europe-an underestimated disease. A GA²LEN study. Allergy. 66:1216–1223. 2011. View Article : Google Scholar : PubMed/NCBI
3	Subspecialty Group of Rhinology, Editorial Board of Chinese Journal of Otorhinolaryngology Head and Neck Surgery; Subspecialty Group of Rhinology, Society of Otorhinolaryngology Head and Neck Surgery, Chinese Medical Association: Guidelines for diagnosis and treatment of chronic rhinosinusitis (2012, Kunming). Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 48:92–94. 2013.PubMed/NCBI
4	Mahoney EJ and Metson R: Palliative care for the patient with refractory chronic rhinosinusitis. Otolaryngol Clin North Am. 42:39–47, vii-viii. 2009. View Article : Google Scholar : PubMed/NCBI
5	Tan BK, Schleimer RP and Kern RC: Perspectives on the etiology of chronic rhinosinusitis. Curr Opin Otolaryngol Head Neck Surg. 18:21–26. 2010. View Article : Google Scholar : PubMed/NCBI
6	Brozek JL, Bousquet J, Baena-Cagnani CE, Bonini S, Canonica GW, Casale TB, van Wijk RG, Ohta K, Zuberbier T, Schünemann HJ, et al: Allergic rhinitis and its impact on asthma (ARIA) guidelines: 2010 revision. J Allergy Clin Immunol. 126:466–476. 2010. View Article : Google Scholar : PubMed/NCBI
7	Okubo K, Kurono Y, Fujieda S, Ogino S, Uchio E, Odajima H and Takenaka H: Japanese Society of Allergology: Japanese guideline for allergic rhinitis 2014. Allergol Int. 63:357–375. 2014. View Article : Google Scholar : PubMed/NCBI
8	Yu C, Cui X, Chen F, Yang J, Qian X and Gao X: Effect of glucocorticoids on aquaporin-1 in guinea pigs with otitis media with effusion. ExpTher Med. 5:1589–1592. 2013.
9	Arbeithuber B, Thuenauer R, Gravogl Y, Balogi Z, Römer W, Sonnleitner A and Tiemann-Boege I: Aquaporin 5 expression in mouse mammary gland cells is not driven by promoter methylation. Biomed Res Int. 2015:4605982015. View Article : Google Scholar : PubMed/NCBI
10	Wang K, Feng YL, Wen FQ, Chen XR, Ou XM, Xu D, Yang J and Deng ZP: Decreased expression of human aquaporin-5 correlated with mucus overproduction in airways of chronic obstructive pulmonary disease. Acta Pharmacol Sin. 28:1166–1174. 2007. View Article : Google Scholar : PubMed/NCBI
11	Wang D, Iwata F, Muraguchi M, Ooga K, Ohmoto Y, Takai M, Mori T and Ishikawa Y: Correlation between salivary secretion and salivary AQP5 levels in health and disease. J Med Invest. 56:(Suppl). S350–S353. 2009. View Article : Google Scholar
12	Yoshimura S, Nakamura H, Horai Y, Nakajima H, Shiraishi H, Hayashi T, Takahashi T and Kawakami A: Abnormal distribution of AQP5 in labial salivary glands is associated with poor saliva secretion in patients with Sjögren's syndrome including neuromyelitis optica complicated patients. Mod Rheumatol. 26:384–390. 2016. View Article : Google Scholar : PubMed/NCBI
13	Frauenfelder C, Woods C, Hussey D, Ooi E, Klebe S and Carney AS: Aquaporin expression profiles in normal sinonasal mucosa and chronic rhinosinusitis. Int Forum Allergy Rhinol. 4:901–908. 2014. View Article : Google Scholar : PubMed/NCBI
14	Shikani AH, Sidhaye VK, Basaraba RJ, Shikani HJ, Alqudah MA, Kirk N, Cope E and Leid JG: Mucosal expression of aquaporin 5 and epithelial barrier proteins in chronic rhinosinusitis with and without nasal polyps. Am J Otolaryngol. 35:377–383. 2014. View Article : Google Scholar : PubMed/NCBI
15	Kmiec Z: J.A. Kiernan. Histological and Histochemical Methods: Theory and Practice. 5th edition, Scion Publishing, 2015, 571 pp. Folia HistochemCytobiol. 54:58–59. 2016.
16	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C (T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
17	Al-Mutairi D and Kilty SJ: Bacterial biofilms and the pathophysiology of chronic rhinosinusitis. Curr Opin Allergy Clin Immunol. 11:18–23. 2011. View Article : Google Scholar : PubMed/NCBI
18	Vickery TW and Ramakrishnan VR: Bacterial pathogens and the microbiome. Otolaryngol Clin North Am. 50:29–47. 2017. View Article : Google Scholar : PubMed/NCBI
19	Sachse F, Becker K and Rudack C: Incidence of staphylococcal colonization and of the 753Q Toll-like receptor 2 variant in nasal polyposis. Am J Rhinol Allergy. 24:e10–e13. 2010. View Article : Google Scholar : PubMed/NCBI
20	Pinchuk IV, Beswick EJ and Reyes VE: Staphylococcal enterotoxins. Toxins (Basel). 2:2177–2197. 2010. View Article : Google Scholar : PubMed/NCBI
21	Singhal D, Foreman A, Jervis-Bardy J and Wormald PJ: Staphylococcus aureus biofilms: Nemesis of endoscopic sinus surgery. Laryngoscope. 121:1578–1583. 2011. View Article : Google Scholar : PubMed/NCBI
22	Ahn SK, Jeon SY, Khalmuratov R, Kim DJ, Kim JP, Park JJ and Hur DG: Rat model of staphylococcal enterotoxin B-induced rhinosinusitis. Clin Exp Otorhinolaryngol. 1:24–28. 2008. View Article : Google Scholar : PubMed/NCBI
23	Liang KL, Jiang RS, Wang J, Shiao JY, Su MC, Hsin CH and Lin JF: Developing a rabbit model of rhinogenic chronic rhinosinusitis. Laryngoscope. 118:1076–1081. 2008. View Article : Google Scholar : PubMed/NCBI
24	Seno S, Ogawa T, Shibayama M, Kouzaki H and Shimizu T: Expression and localization of aquaporin 1, 2, 3, 4 and 5 in human nasal mucosa. Am J Rhinol Allergy. 26:167–171. 2012. View Article : Google Scholar : PubMed/NCBI
25	Ben Y, Chen J, Zhu R, Gao L and Bai C: Upregulation of AQP3 and AQP5 induced by dexamethasone and ambroxol in A549 cells. Respir Physiol Neurobiol. 161:111–118. 2008. View Article : Google Scholar : PubMed/NCBI
26	Dong C, Wang G, Li B, Xiao K, Ma Z, Huang H, Wang X and Bai C: Anti-asthmatic agents alleviate pulmonary edema by upregulating AQP1 and AQP5 expression in the lungs of mice with OVA-induced asthma. Respir Physiol Neurobiol. 181:21–28. 2012. View Article : Google Scholar : PubMed/NCBI