Increased Runx2 expression associated with enhanced Wnt signaling in PDLLA internal fixation for fracture treatment

  • Authors:
    • Zhuoyan Ling
    • Lei Wu
    • Gaolong Shi
    • Li Chen
    • Qirong Dong
  • View Affiliations

  • Published online on: March 10, 2017     https://doi.org/10.3892/etm.2017.4216
  • Pages: 2085-2093
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Abstract

Poly-D-L lactide (PDLLA) biodegradable implants to heal fractures are widely applied in orthopedic surgeries. However, whether the process of fracture healing is regulated differently when PDLLA is used compared with traditional metal materials remains unclear. Runt‑related transcription factor 2 (Runx2) and canonical Wnt signaling are essential and may interact reciprocally in the regulation of osteogenesis during bone repair. In the present study, a rat femoral open osteotomy model was used to compare the curative efficacy of a PDLLA rod and Kirschner wire under intramedullary fixation for fracture treatment. The dynamic expression of Runx2 and key components of the canonical Wnt signaling in callus tissue during fracture healing was also investigated. The results of the current study indicate that at weeks 4 and 6 following fixation, the callus bone structural parameters of microCT were significantly improved by PDLLA rod compared to that of Kirschner wire. In addition, at weeks 4 and 6 after fixation, the protein and mRNA expression of Runx2 and the positive regulators of canonical Wnt signaling, such as Wnts and β‑catenin, were significantly increased. However, the protein and mRNA expression levels of the negative regulators of canonical Wnt signaling, such as glycogen synthase kinase‑3β, were significantly decreased in callus tissue when treated with PDLLA rod compared with Kirschner wire. Collectively, these data indicate that compared to the traditional metal material, using PDLLA internal fixation for fracture treatment may further improve bone formation, which is associated with the increased expression of Runx2 and the enhancement of canonical Wnt signaling.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ling Z, Wu L, Shi G, Chen L and Dong Q: Increased Runx2 expression associated with enhanced Wnt signaling in PDLLA internal fixation for fracture treatment. Exp Ther Med 13: 2085-2093, 2017
APA
Ling, Z., Wu, L., Shi, G., Chen, L., & Dong, Q. (2017). Increased Runx2 expression associated with enhanced Wnt signaling in PDLLA internal fixation for fracture treatment. Experimental and Therapeutic Medicine, 13, 2085-2093. https://doi.org/10.3892/etm.2017.4216
MLA
Ling, Z., Wu, L., Shi, G., Chen, L., Dong, Q."Increased Runx2 expression associated with enhanced Wnt signaling in PDLLA internal fixation for fracture treatment". Experimental and Therapeutic Medicine 13.5 (2017): 2085-2093.
Chicago
Ling, Z., Wu, L., Shi, G., Chen, L., Dong, Q."Increased Runx2 expression associated with enhanced Wnt signaling in PDLLA internal fixation for fracture treatment". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2085-2093. https://doi.org/10.3892/etm.2017.4216