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Article Open Access

Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis

  • Authors:
    • Jianlin Liu
    • Juan Dai
    • Yansong Wang
    • Siyu Lai
    • Suwen Wang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Shenzhen Hospital of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong 518033, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2325-2331
    |
    Published online on: March 17, 2017
       https://doi.org/10.3892/etm.2017.4234
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Abstract

We studied the significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis (TMJOA). Fifteen 8-week-old female Sprague-Dawley rats were selected to establish TMJOA models of gradually induced occlusal disorders. Five rats were sacrificed at 4, 8 and 16 weeks, and histological exam was conducted along with micro-computed tomography observation on the condyle specimen. The distribution and number of new blood vessels breaking were observed through the tidemark through CD34 immunofluorescence staining. The proliferation of chondrocytes were detected through Ki67 immunohistochemical staining, and the differentiation functions of chondrocytes were observed through PTHrP and IHH immunohistochemical staining. The degradation functions of cartilage matrix were observed through matrix metalloproteinase (MMP)-9 immunohistochemical staining to detect the expression of vascular growth promotion and inhibition factors with vascular endothelial growth factor (VEGF), CTGF and CHM-1 immunohistochemical staining and screen differentially expressed genes through gene chip analysis method. It was found that the condyle tissue full thickness, fiber layer thickness and calcified cartilage layer thickness were significantly increased with time (P<0.05). Bone mineral density, trabecular thickness and Tb.Sp were also increased significantly with time, BS/BV and trabecular number were decreased significantly with time (P<0.05). The new blood vessels reached the deep layer of calcified cartilage until the tide line was broken and non-calcified cartilage was invaded. The number of vessels were increased significantly with time (P<0.05). Ki67, PTHrP and IHH-positive rates were increased signi­ficantly (P<0.05). MMP-9, VEGF, CTGF and CHM-1 were increased significantly (P<0.05). VEGF, CTGF and CHM-1 mRNA were upregulated differentially with the expressed genes. In conclusion, the new blood vessels may be important in the pathogenesis of TMJOA.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Dai J, Wang Y, Lai S and Wang S: Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis. Exp Ther Med 13: 2325-2331, 2017.
APA
Liu, J., Dai, J., Wang, Y., Lai, S., & Wang, S. (2017). Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis. Experimental and Therapeutic Medicine, 13, 2325-2331. https://doi.org/10.3892/etm.2017.4234
MLA
Liu, J., Dai, J., Wang, Y., Lai, S., Wang, S."Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis". Experimental and Therapeutic Medicine 13.5 (2017): 2325-2331.
Chicago
Liu, J., Dai, J., Wang, Y., Lai, S., Wang, S."Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2325-2331. https://doi.org/10.3892/etm.2017.4234
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Dai J, Wang Y, Lai S and Wang S: Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis. Exp Ther Med 13: 2325-2331, 2017.
APA
Liu, J., Dai, J., Wang, Y., Lai, S., & Wang, S. (2017). Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis. Experimental and Therapeutic Medicine, 13, 2325-2331. https://doi.org/10.3892/etm.2017.4234
MLA
Liu, J., Dai, J., Wang, Y., Lai, S., Wang, S."Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis". Experimental and Therapeutic Medicine 13.5 (2017): 2325-2331.
Chicago
Liu, J., Dai, J., Wang, Y., Lai, S., Wang, S."Significance of new blood vessels in the pathogenesis of temporomandibular joint osteoarthritis". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2325-2331. https://doi.org/10.3892/etm.2017.4234
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