Hemophagocytic lymphohistiocytosis in a patient with human immunodeficiency virus infection: A case report

Introduction

Hemophagocytic lymphohistiocytosis (HLH), also termed hemophagocytic syndrome, is a state of severe, life-threatening inflammation caused by an extreme, prolonged and ineffective immune response (1). HLH may occur as a genetic or sporadic disorder and, though seen as an inherited condition affecting primarily a pediatric population, HLH may occur at any age. Furthermore, HLH may be encountered in association with a variety of underlying diseases and is considered a hyperinflammatory syndrome with high mortality, even with appropriate treatment (1,2). The syndrome is typically induced by overactive macrophages from the bone marrow or lymph tissue, which phagocytose erythrocytes, leukocytes and platelets (3,4). The clinical characteristics of HLH include fever for an extended duration, hepatosplenomegaly, cytopenia and hemophagocytosis due to activated macrophages. HLH may be classified as either primary familial HLH, where the cause is predominantly genetic, or secondary HLH, which is typically attributed to infections. More commonly, viral infections such as Epstein-Barr virus (EBV) may trigger secondary HLH; however, autoimmune diseases and malignancies have also been demonstrated to have a role in secondary HLH (5). Previous reports of HLH have focused on children and malignancy-related diseases (6,7). HLH in a patient with human immunodeficiency virus (HIV) infection has rarely been investigated previously (8). The present study demonstrates an uncommon case of HLH in combination with HIV infection in a patient, who eventually succumbed to severe infection and multiple organ failure following refusal of medical treatment.

Case report

General information and medical examination

In May 2015, a 42-year-old male presented to the First Affiliated Hospital of Nanchang University (Nanchang, China) with a medical history of high fever experienced for 30 days and sudden breathing difficulty for 1 day. The family of the patient complained that 1 month ago, without apparent inducement, he developed recurrent fever, headache, dizziness, nausea, vomiting, chest tightness and shortness of breath. The patient was admitted to a local county-level hospital and given anti-infection and anti-flu treatment (specific drug use is unknown). The patient experienced recurrence of high fever for 4–5 days after an initial improvement in fever symptoms for 2–3 days. This fluctuation in fever symptoms persisted for several weeks.

Routine blood examination and bone marrow smears were performed to rule out infectious diseases. The complete blood count indicated pancytopenia: White blood cell count, 0.30×109/l (normal range, 4–10×109/l); red blood cell count, 3.22×1012/l (normal range, 4.09–5.71×1012/l); hemoglobin levels, 81 g/l (normal range, 131–172 g/l); and platelet count, 29×109/l (normal range, 85–300×109/l). Furthermore, increased levels of serum ferritin were exhibited (>2,000.0 µg/l; normal range, 30–400 µg/l) and soluble interleukin-2 receptor levels were increased (44,000 pg/ml; normal levels, <6,400 pg/ml). Additional laboratory findings were as follows: White blood cell count, 0.30×109/l with 20.1% neutrophils, 53.3% lymphocytes and 23.3% monocytes; aspartate aminotransferase (AST), 721 U/l; alanine aminotransferase (ALT), 130 U/l; lactate dehydrogenase, 963 mg/dl; alkaline phosphatase, 235 U/l; and γ-glutamyltranspeptidase, 191 U/l. A reduced level of total protein was observed (55.2 g/l; normal range, 60–78 g/l), albumin was 21.2 g/l (normal range 34–48 g/l) and there was an increased level of C-reactive protein (94.60 mg/l; normal range, 0–8 mg/l). D-Dimer levels were high (6,779 µg/l; normal range, 0–300 µg/l). Serum antibody to EBV, tubercle bacillus and hemococcidium were negative.

The results of computed tomography imaging examination of the upper abdomen indicated infection in both lungs, fatty liver, splenomegaly and retroperitoneal multiple enlarged lymph nodes. The findings indicated a preliminary diagnosis of infectious multiple organ dysfunction syndrome, pulmonary infection, and hypoproteinemia.

Results of bone marrow and peripheral blood smear

Bone marrow fluid was obtained by bone marrow aspiration. A bone marrow smear and peripheral blood smear was performed using Wright and Giemsa staining (Baso 4017 kit; Baso Diagnostics Inc., Zhuhai, China). Both samples were observed under a microscope (ECLIPSE Ci; Nikon Corp., Tokyo, Japan) with a magnification of ×1,000. Karyocyte cells in the bone marrow were decreased slightly and the proportion of lymphocytes was high (46%; normal range, <20%). The proportion of granulocyte and erythrocytes was borderline normal; however, the mature stage of the granulocyte was markedly decreased. Macrophages were exhibited at an elevated percentage of 8.5% and hemophagocytosis was clearly observed. Cellular size of the macrophages ranged between 20 and 55 µm in diameter and their shapes were rounded and irregular with irregular margins accompanying pseudopodia. The macrophage possessed abundant cytoplasm, indicated as grey blue or light grey, which engulfed a complete blood cell and fungi or other unknown microbes (Fig. 1A and B). These macrophages often exhibited single eccentric nuclei and possessed a rounded or oval nucleus and loose mesh nuclear chromatin. The morphology and classification of white blood cells in the peripheral blood were not abnormal and the distribution of fungi and bacteria was observed in the cytoplasm (Fig. 1C).

Figure 1. (A and B) Bone marrow aspirate smear and (C) peripheral blood smear demonstrated phagocytosis of macrophages, which were irregular in shape, leading to the diagnosis of hemophagocytic lymphohistiocytosis (Wright and Giemsa staining; magnification, ×1,000).

Disease progression and HIV diagnosis

The patient was hospitalized for three days and treated with anti-infection and anti-flu treatment (specific agents unknown). The levels of peripheral blood hemoglobin, percentage of monocyte cells and platelets decreased and the serum total protein and albumin levels gradually declined over the course of these three days. Furthermore, ALT, AST, urea, creatinine and plasma endotoxin levels were rapidly increased (Table I). On the third day of hospitalization, the patient appeared delirious, moist rales were heard in both lungs and multiple organ failure and scattered ecchymosis were exhibited. The predominant concern was the multiple organ failure, acute respiratory failure, acute liver failure, blood coagulation disorder, pulmonary infection and AIDS. Blood specimens were screened for AIDS and sent for examination to the Center for Disease Control of Jiangxi Province (CDC) to confirm the patient was infected with HIV. Following three days, the HIV test from CDC confirmed a positive diagnosis. The patient's family refused medical treatment on the third day of hospitalization. The patient subsequently succumbed to infectious multiple organ failure in his home.

Table I. Routine blood examination results on each day of hospitalization.

Table I.

Routine blood examination results on each day of hospitalization.

Variable	First day	Second day	Third day
White blood cell (x109/l)	0.30	0.44	0.40
Neutrophils (%)	20.1	20.4	17.6
Lymphocytes (%)	53.3	61.4	52.5
Monocytes (%)	23.3	18.2	16.8
Red blood cells (x1012/l)	3.22	3.14	2.74
Hemoglobin (g/l)	81	78	70
Platelets (x109/l)	29	19	13
Alanine aminotransferase (U/l)	130	147	169
Aspartate aminotransferase (U/l)	721	762	943
Total protein (g/l)	55.2	42.3	38.5
Albumin (g/l)	21.2	20.5	19.5
Globulin (g/l)	34.0	21.8	19.0
Ratio of albumin to globulin	0.62	0.94	1.03
γ-glutamyltranspeptidase (U/l)	191	156	122
Creatinine (mmol/l)	77.7	293.0	285.7
Urea (µmol/l)	4.2	7.5	10.3
Uric acid (µmol/l)	118	255	248
Creatine kinase (U/l)	–	199	573
Amylase (U/l)	–	116	123
Plasma endotoxin (U/ml)	1.23	3.06	23.58

Discussion

Fever, cytopenia of at least two cell types, hypertriglyceridemia and/or hypofibrinogenemia, hyperferritinemia (>500 µg/l), hemophagocytosis, elevated levels of serum CD25, decreased levels of NK cell activity and splenomegaly are a set of symptoms, of which five are required to suggest secondary HLH, according to the guidelines of the International Histiocyte Society (9). Based on the clinical and laboratory findings of fever, splenomegaly, cytopenias, hemophagocytosis in the bone marrow, hyperferritinemia and raised serum CD25 levels, a diagnosis of secondary HLH was therefore established.

The association between HLH and HIV is not well understood, as few such cases exist. HIV infection may contribute to the development of HLH, possibly through mechanisms related to CD4+ cells (predominantly T lymphocytes, monocytes, macrophages and dendritic cells) (10). Following HIV infection, viral incorporation of CD4+ cell DNA, combined with an overwhelming trophic response of relapsed T cell lymphoma may overstimulate the immune system, leading to HLH (11). At present, the pathogenesis of HLH has been suggested to be associated with a deficiency in cytolytic activity, which results from stimulation of lymphocytes and histiocytes (12). This uncontrolled immune response causes enhanced production of pro-inflammatory cytokines and major histocompatibility complex I and II molecules from macrophages as well as the expansion of inflammatory monocytes. Subsequently, this heightened inflammatory response causes necrosis, organ failure and promotes the proliferation and phagocytic activity of histiocytes (13). In the present case, the macrophages appeared to exhibit hyperactivity, which was indicated by the markedly elevated percentage of monocytes detected in the peripheral blood and the markedly increased percentage of macrophages detected in the bone marrow. Furthermore, hemophagocytosis in macrophages was clearly observed from the bone marrow and peripheral blood smear.

HLH is difficult to treat and is typically associated with a high morbidity and mortality rate (3,14). HLH therapy must target the suppression the hyper-stimulated immune system via abolishing activated CD8+ T lymphocytes or macrophages, in addition to treating any existing HLH triggers, including infections, autoimmune diseases and malignancies (14).

The present case study demonstrates that HLH associated with HIV infection is a severe disease with a poor prognosis and may result in infection, multiple organ failure and mortality if the correct treatment is not administered in a timely-manner. In the present case, laboratory testing, including cell morphological examination and hemophagocytosis detected in the bone marrow, indicated that HLH was associated with HIV infection. Therefore, laboratory physicians should consider HLH and identify the possible cause (infections, autoimmune disease and malignancies) once intense hemophagocytosis is detected in the bone marrow.

References