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Article

Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease

  • Authors:
    • Jianhua Yang
    • Bowei Ju
    • Yao Yan
    • Huanhuan Xu
    • Shanshan Wu
    • Dandan Zhu
    • Dandan Cao
    • Junping Hu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China
  • Pages: 2423-2428
    |
    Published online on: March 22, 2017
       https://doi.org/10.3892/etm.2017.4254
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Abstract

The present study aimed to investigate the neuroprotective effects of phenylethanol glycosides (PhGs) on H2O2‑ and β‑amyloid peptide (Aβ)1‑42‑induced injury of PC12 cells as an in vitro model of Alzheimer's disease (AD). The optimal induction conditions were established through screening of various incubation times and concentrations. PC12 cells were treated with 0.5 µM Aβ1‑42 and H2O2 in the presence of PhGs for 24 h and the cell viability was then evaluated by an MTT assay; lactate dehydrogenase (LDH) release and malondialdehyde (MDA) content were also measured. The optimal conditions for establishing the AD model were the treatment of PC12 cells with 0.5 µM Aβ1‑42 for 48 h, or with 25 µM H2O2 dissolved in DMEM with PBS. PhGs at concentrations of 5, 25 and 50 µg/ml increased the viability and decreased LDH and MDA release by PC12 cells injured with Aβ1‑42 or H2O2. In conclusion, the model of Aβ1‑42‑ and H2O2‑induced PC12 cell injury was successfully established. PhGs were shown to have a significant neuroprotective effect against Aβ1‑42‑ or H2O2‑induced cell injury.
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Copy and paste a formatted citation
Spandidos Publications style
Yang J, Ju B, Yan Y, Xu H, Wu S, Zhu D, Cao D and Hu J: Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease. Exp Ther Med 13: 2423-2428, 2017.
APA
Yang, J., Ju, B., Yan, Y., Xu, H., Wu, S., Zhu, D. ... Hu, J. (2017). Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease. Experimental and Therapeutic Medicine, 13, 2423-2428. https://doi.org/10.3892/etm.2017.4254
MLA
Yang, J., Ju, B., Yan, Y., Xu, H., Wu, S., Zhu, D., Cao, D., Hu, J."Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease". Experimental and Therapeutic Medicine 13.5 (2017): 2423-2428.
Chicago
Yang, J., Ju, B., Yan, Y., Xu, H., Wu, S., Zhu, D., Cao, D., Hu, J."Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2423-2428. https://doi.org/10.3892/etm.2017.4254
Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Ju B, Yan Y, Xu H, Wu S, Zhu D, Cao D and Hu J: Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease. Exp Ther Med 13: 2423-2428, 2017.
APA
Yang, J., Ju, B., Yan, Y., Xu, H., Wu, S., Zhu, D. ... Hu, J. (2017). Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease. Experimental and Therapeutic Medicine, 13, 2423-2428. https://doi.org/10.3892/etm.2017.4254
MLA
Yang, J., Ju, B., Yan, Y., Xu, H., Wu, S., Zhu, D., Cao, D., Hu, J."Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease". Experimental and Therapeutic Medicine 13.5 (2017): 2423-2428.
Chicago
Yang, J., Ju, B., Yan, Y., Xu, H., Wu, S., Zhu, D., Cao, D., Hu, J."Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2423-2428. https://doi.org/10.3892/etm.2017.4254
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