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Article

Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis

  • Authors:
    • Bo Zhan
    • Chuize Kong
    • Zhe Zhang
    • Xiao Dong
    • Naiwen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 2473-2479
    |
    Published online on: March 23, 2017
       https://doi.org/10.3892/etm.2017.4258
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Abstract

Kidney cancer is among the most important causes of cancer-associated mortality worldwide. The present study aimed to evaluate protein kinase C α (PKCα) expression in kidney cancer tissues and cell lines, and its significance in apoptosis and migration. Expression of PKCα was analyzed using quantitative polymerase chain reaction and western blotting. In addition, the inhibitor of PKCα (calphostin C and GO6976) was used to treat kidney cancer cells. The ACHN cell line was generated with PKCα-small-interfering RNA (siRNA) and a stable expression of PKCα, in order to facilitate the analysis of apoptosis and migration of PKCα during knockdown and inactivation. Flow cytometry was used to determine the rates of apoptosis. Immunohistochemical staining was used to identify the localization of PKCα in renal clear cell carcinoma and normal sections. PKCα expression in normal tissues was found to be greater than in cancerous tissues. Furthermore, apoptosis was not promoted with PKCα inhibitors or PKCα-siRNA treatment, and a decrease of the migration ability was observed following transfection with PKCα-dominant negative. The results indicated that inhibition of PKCα might not contribute to apoptosis progression in kidney carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhan B, Kong C, Zhang Z, Dong X and Zhang N: Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis. Exp Ther Med 13: 2473-2479, 2017.
APA
Zhan, B., Kong, C., Zhang, Z., Dong, X., & Zhang, N. (2017). Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis. Experimental and Therapeutic Medicine, 13, 2473-2479. https://doi.org/10.3892/etm.2017.4258
MLA
Zhan, B., Kong, C., Zhang, Z., Dong, X., Zhang, N."Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis". Experimental and Therapeutic Medicine 13.5 (2017): 2473-2479.
Chicago
Zhan, B., Kong, C., Zhang, Z., Dong, X., Zhang, N."Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2473-2479. https://doi.org/10.3892/etm.2017.4258
Copy and paste a formatted citation
x
Spandidos Publications style
Zhan B, Kong C, Zhang Z, Dong X and Zhang N: Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis. Exp Ther Med 13: 2473-2479, 2017.
APA
Zhan, B., Kong, C., Zhang, Z., Dong, X., & Zhang, N. (2017). Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis. Experimental and Therapeutic Medicine, 13, 2473-2479. https://doi.org/10.3892/etm.2017.4258
MLA
Zhan, B., Kong, C., Zhang, Z., Dong, X., Zhang, N."Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis". Experimental and Therapeutic Medicine 13.5 (2017): 2473-2479.
Chicago
Zhan, B., Kong, C., Zhang, Z., Dong, X., Zhang, N."Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2473-2479. https://doi.org/10.3892/etm.2017.4258
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