Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May-2017 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2017 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function

  • Authors:
    • Qing Zhou
    • Qing Deng
    • Bo Hu
    • Yi‑Jia Wang
    • Jin‑Ling Chen
    • Jing‑Jing Cui
    • Sheng Cao
    • Hong‑Ning Song
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound Imaging, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2293-2303
    |
    Published online on: March 28, 2017
       https://doi.org/10.3892/etm.2017.4270
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to construct targeted cationic microbubbles (TCMBs) by synthesizing cationic microbubbles conjugated to an intercellular adhesion molecule‑1 (ICAM‑1) antibody, and then to use the TCMBs to deliver the angiopoietin‑1 (Ang‑1) gene into infarcted heart tissue using ultrasound‑mediated microbubble destruction. It was hypothesized that the TCMBs would accumulate in higher numbers than non‑targeted cationic microbubbles (CMBs) in the infarcted heart, and would therefore increase the efficiency of targeted Ang‑1 gene transfection and promote angiogenesis. The results of the study demonstrated that the ability of TCMBs to target inflammatory endothelial cells was 18.4‑fold higher than that of the CMBs in vitro. The accumulation of TCMBs was greater than that of CMBs in TNF‑α‑stimulated human umbilical cord veins, indicated by a 212% higher acoustic intensity. In vivo, the TCMBs specifically accumulated in the myocardial infarct area in a rabbit model. Three days after ultrasound microbubble‑mediated gene transfection, Ang‑1 protein expression in the TCMB group was 2.7‑fold higher than that of the CMB group. Angiogenesis, the thickness of the infarct region and the heart function of the TCMB group were all significantly improved compared with those in the CMB and control groups at 4 weeks following gene transfection (all P<0.01). Therefore, the results of the current study demonstrate that ultrasound‑mediated TCMB destruction effectively delivered the Ang‑1 gene to the infarcted myocardium, resulting in improved cardiac morphology and function in the animal model. Ultrasound‑mediated TCMB destruction is a promising strategy for improving gene therapy in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Henry TD, Satran D, Hodges JS, Johnson RK, Poulose AK, Campbell AR, Garberich RF, Bart BA, Olson RE, Boisjolie CR, et al: Long-term survival in patients with refractory angina. Eur Heart J. 34:2683–2688. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Lassaletta AD, Chu LM and Sel FW: Therapeutic neovascularization for coronary disease: Current state and future prospects. Basic Res Cardiol. 106:897–909. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Cochain C, Channon KM and Silvestre JS: Angiogenesis in the infarcted myocardium. Antioxid Redox Signal. 18:1100–1113. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Katz MG, Fargnoli AS, Williams RD and Bridges CR: The road ahead: Working towards effective clinical translation of myocardial gene therapies. Ther Deliv. 5:39–51. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Katz MG, Fargnoli AS, Pritchette LA and Bridges CR: Gene delivery technologies for cardiac applications. Gene Ther. 19:659–669. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Cool SK, Geers B, Lentacker I, De Smedt SC and Sanders NN: Enhancing nucleic acid delivery with ultrasound and microbubbles. Methods Mol Biol. 948:195–204. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Geis NA, Katus HA and Bekeredjian R: Microbubbles as a vehicle for gene and drug delivery: Current clinical implications and future perspectives. Curr Pharm Des. 18:2166–2183. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Ma J, DU LF, Chen M, Wang HH, Xing LX, Jing LF and Li YH: Drug-loaded nano-microcapsules delivery system mediated by ultrasound-targeted microbubble destruction: A promising therapy method. Biomed Rep. 1:506–510. 2013.PubMed/NCBI

9 

Smith AH, Fujii H, Kuliszewski MA and Leong-Poi H: Contrast ultrasound and targeted microbubbles: Diagnostic and therapeutic applications for angiogenesis. J Cardiovasc Transl Res. 4:404–415. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Liao YY, Chen ZY, Wang YX, Lin Y, Yang F and Zhou QL: New progress in angiogenesis therapy of cardiovascular disease by ultrasound targeted microbubble destruction. Biomed Res Int. 2014:8729842014. View Article : Google Scholar : PubMed/NCBI

11 

Fujii H, Sun Z, Li SH, Wu J, Fazel S, Weisel RD, Rakowski H, Lindner J and Li RK: Ultrasound-targeted gene delivery induces angiogenesis after a myocardial infarction in mice. JACC Cardiovasc Imaging. 2:869–879. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Castle J, Butts M, Healey A, Kent K, Marino M and Feinstein SB: Ultrasound-mediated targeted drug delivery: Recent success and remaining challenges. Am J Physiol Heart Circ Physiol. 304:H350–H357. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Delalande A, Postema M, Mignet N, Midoux P and Pichon C: Ultrasound and microbubble-assisted gene delivery: Recent advances and ongoing challenges. Ther Deliv. 3:1199–1215. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Yang H, Xiong XY, Zhang L, Wu C and Liu Y: Adhesion of bio-functionalized ultrasound microbubbles to endothelial cells by targeting to vascular cell adhesion molecule-1 under shear flow. Int J Nanomedicine. 6:2043–2051. 2011.PubMed/NCBI

15 

Murciano JC, Muro S, Koniaris L, Christofidou-Solomidou M, Harshaw DW, Albelda SM, Granger DN, Cines DB and Muzykantov VR: ICAM-directed vascular immunotargeting of antithrombotic agents to the endothelial luminal surface. Blood. 101:3977–3984. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Yan Y, Liao Y, Yang L, Wu J, Du J, Xuan W, Ji L, Huang Q, Liu Y and Bin J: Late-phase detection of recent myocardial ischaemia using ultrasound molecular imaging targeted to intercellular adhesion molecule-1. Cardiovasc Res. 89:175–183. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Morisada T, Kubota Y, Urano T, Suda T and Oike Y: Angiopoietins and angiopoietin-like proteins in angiogenesis. Endothelium. 13:71–79. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Zhang H, Yuan YL, Wang Z, Jiang B, Zhang CS, Wang Q, Xu XH, Dong HY and Zhang ZM: Sequential, timely and controlled expression of hVEGF165 and Ang-1 effectively improves functional angiogenesis and cardiac function in vivo. Gene Ther. 20:893–900. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Paul A, Binsalamah ZM, Khan AA, Abbasia S, Elias CB, Shum-Tim D and Prakash S: A nanobiohybrid complex of recombinant baculovirus and Tat/DNA nanoparticles for delivery of Ang-1 transgene in myocardial infarction therapy. Biomaterials. 32:8304–8318. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Fagiani E and Christofori G: Angiopoietins in angiogenesis. Cancer Lett. 328:18–26. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Xie A, Belcik T, Qi Y, Morgan TK, Champaneri SA, Taylor S, Davidson BP, Zhao Y, Klibanov AL, Kuliszewski MA, et al: Ultrasound-mediated vascular gene transfection by cavitation of endothelial-targeted cationic microbubbles. JACC Cardiovasc Imaging. 5:1253–1262. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Yan P, Chen KJ, Wu J, Sun L, Sung HW, Weisel RD and Li RK: The use of MMP2 antibody-conjugated cationic microbubble to target the ischemic myocardium, enhance Timp3 gene transfection and improve cardiac function. Biomaterials. 35:1063–873. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Weidner N: Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors. Breast Cancer Res Treat. 36:169–180. 1995. View Article : Google Scholar : PubMed/NCBI

24 

Delalande A, Kotopoulis S, Postema M, Midoux P and Pichon C: Sonoporation: Mechanistic insights and ongoing challenges for gene transfer. Gene. 525:191–199. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Panje CM, Wang DS, Pysz MA, Paulmurugan R, Ren Y, Tranquart F, Tian L and Willmann JK: Ultrasound-mediated gene delivery with cationic versus neutral microbubbles: Effect of DNA and microbubble dose on in vivo transfection efficiency. Theranostics. 2:1078–1091. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Luo D and Saltzman WM: Enhancement of transfection by physical concentration of DNA at the cell surface. Nat Biotechnol. 18:893–895. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Wang DS, Panje C, Pysz MA, Paulmurugan R, Rosenberg J, Gambhir SS, Schneider M and Willmann JK: Cationic versus neutral microbubbles for ultrasound-mediated gene delivery in cancer. Radiology. 264:721–732. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Wang X, Liang HD, Dong B, Lu QL and Blomley MJ: Gene transfer with microbubble ultrasound and plasmid DNA into skeletal muscle of mice: Comparison between commercially available microbubble contrast agents. Radiology. 237:224–229. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Zhou J, Wang Y, Xiong Y, Wang H, Feng Y and Chen J: Delivery of TFPI-2 using ultrasound with a microbubble agent (SonoVue) inhibits intimal hyperplasia after balloon injury in a rabbit carotid artery model. Ultrasound Med Biol. 36:1876–1883. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Lentacker I, De Geest BG, Vandenbroucke RE, Peeters L, Demeester J, De Smedt SC and Sanders NN: Ultrasound-responsive polymer-coated microbubbles that bind and protect DNA. Langmuir. 22:7273–7278. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Ju J, Huan ML, Wan N, Hou YL, Ma XX, Jia YY, Li C, Zhou SY and Zhang BL: Cholesterol derived cationic lipids as potential non-viral gene delivery vectors and their serum compatibility. Bioorg Med Chem Lett. 26:2401–2407. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Sun L, Huang CW, Wu J, Chen KJ, Li SH, Weisel RD, Rakowski H, Sung HW and Li RK: The use of cationic microbubbles to improve ultrasound-targeted gene delivery to the ischemic myocardium. Biomaterials. 34:2107–2116. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Wang X, Hagemeyer CE, Hohmann JD, Leitner E, Armstrong PC, Jia F, Olschewski M, Needles A, Peter K and Ahrens I: Novel single-chain antibody-targeted microbubbles for molecular ultrasound imaging of thrombosis: Validation of a unique noninvasive method for rapid and sensitive detection of thrombi and monitoring of success or failure of thrombolysis in mice. Circulation. 125:3117–3126. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Warram JM, Sorace AG, Mahoney M, Samuel S, Harbin B, Joshi M, Martin A, Whitworth L, Hoyt K and Zinn KR: Biodistribution of P-selectin targeted microbubbles. J Drug Target. 22:387–394. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Leong-Poi H, Christiansen J, Klibanov AL, Kaul S and Lindner JR: Noninvasive assessment of angiogenesis by ultrasound and microbubbles targeted to alpha (v)-integrinss. Circulation. 107:455–460. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Dabek J, Ligus J and Szota J: Oligonucleotide microarray and QRT-PCR study of adhesion protein gene expression in acute coronary syndrome patients. Inflammation. 33:398–407. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Lawson C and Wolf S: ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61:22–32. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Mulvihill NT and Foley JB: Inflammation in acute coronary syndromes. Heart. 87:201–204. 2002. View Article : Google Scholar : PubMed/NCBI

39 

Benson V, McMahon AC and Lowe HC: ICAM-1 in acute myocardial infarction: A potential therapeutic target. Curr Mol Med. 7:219–227. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou Q, Deng Q, Hu B, Wang YJ, Chen JL, Cui JJ, Cao S and Song HN: Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function. Exp Ther Med 13: 2293-2303, 2017.
APA
Zhou, Q., Deng, Q., Hu, B., Wang, Y., Chen, J., Cui, J. ... Song, H. (2017). Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function. Experimental and Therapeutic Medicine, 13, 2293-2303. https://doi.org/10.3892/etm.2017.4270
MLA
Zhou, Q., Deng, Q., Hu, B., Wang, Y., Chen, J., Cui, J., Cao, S., Song, H."Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function". Experimental and Therapeutic Medicine 13.5 (2017): 2293-2303.
Chicago
Zhou, Q., Deng, Q., Hu, B., Wang, Y., Chen, J., Cui, J., Cao, S., Song, H."Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2293-2303. https://doi.org/10.3892/etm.2017.4270
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Q, Deng Q, Hu B, Wang YJ, Chen JL, Cui JJ, Cao S and Song HN: Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function. Exp Ther Med 13: 2293-2303, 2017.
APA
Zhou, Q., Deng, Q., Hu, B., Wang, Y., Chen, J., Cui, J. ... Song, H. (2017). Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function. Experimental and Therapeutic Medicine, 13, 2293-2303. https://doi.org/10.3892/etm.2017.4270
MLA
Zhou, Q., Deng, Q., Hu, B., Wang, Y., Chen, J., Cui, J., Cao, S., Song, H."Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function". Experimental and Therapeutic Medicine 13.5 (2017): 2293-2303.
Chicago
Zhou, Q., Deng, Q., Hu, B., Wang, Y., Chen, J., Cui, J., Cao, S., Song, H."Ultrasound combined with targeted cationic microbubble‑mediated angiogenesis gene transfection improves ischemic heart function". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2293-2303. https://doi.org/10.3892/etm.2017.4270
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team