Peucedanum japonicum Thunb. ethanol extract suppresses RANKL‑mediated osteoclastogenesis

  • Authors:
    • Jeong‑Mi Kim
    • Munkhsoyol Erkhembaatar
    • Guem‑San Lee
    • Jin‑Hyun Lee
    • Eun‑Mi Noh
    • Minok Lee
    • Hyun‑Kyung Song
    • Choong Hun Lee
    • Kang‑Beom Kwon
    • Min Seuk Kim
    • Young‑Rae Lee
  • View Affiliations

  • Published online on: May 19, 2017     https://doi.org/10.3892/etm.2017.4480
  • Pages: 410-416
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Abstract

The constituents of Peucedanum japonicum Thunb. (PJ) exhibit biological and pharmacological activities, including anti‑obesity, anti‑oxidant and anti‑allergic activities. The aim of the present study was to examine in vitro effects of PJ in RANKL‑induced signaling pathways, which determine osteoclast differentiation. PJ ethanol extract (PEE) exhibited anti‑osteoporotic activity by disrupting the phospholipase C (PLC)‑Ca2+‑c‑Fos/cAMP response element‑binding protein (CREB)‑nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) signaling pathway during osteoclastogenesis. Murine bone marrow‑derived macrophages (BMMs) were cultured and used to determine the effects of PJ in the receptor activator of nuclear factor κB ligand (RANKL)‑mediated osteoclastogenesis. The effects of PEE in the RANKL‑mediated signaling cascade were evaluated using a standard in vitro osteoclastogenesis system. PEE treatment of BMMs significantly reduced the number of RANKL‑mediated tartrate resistant acid phosphatase (TRAP)‑positive multinucleated cells (P<0.05 for 5 and 10 µg/ml PEE, P<0.01 for 25 and 50 µg/ml PEE), without cytotoxic effects. Furthermore, the expression of differentiation‑related marker genes, including TRAP, Oscar, Cathepsin K, dendrocyte expressed seven transmembrane protein, ATPase H+ Transporting V0 Subunit D2 and NFATc1, were markedly suppressed. PEE induced a transient increase in free cytoplasmic Ca2+ ([Ca2+]i) mobilization via voltage‑gated Ca2+ channels and PLC‑sensitive pathways. Transient [Ca2+]i increase consequently resulted in the suppression of c‑Fos, CREB and NFATc1 activities. These findings highlight the potential use of PJ in treating bone disorders caused by osteoclast overgrowth.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kim JM, Erkhembaatar M, Lee GS, Lee JH, Noh EM, Lee M, Song HK, Lee CH, Kwon KB, Kim MS, Kim MS, et al: Peucedanum japonicum Thunb. ethanol extract suppresses RANKL‑mediated osteoclastogenesis. Exp Ther Med 14: 410-416, 2017
APA
Kim, J., Erkhembaatar, M., Lee, G., Lee, J., Noh, E., Lee, M. ... Lee, Y. (2017). Peucedanum japonicum Thunb. ethanol extract suppresses RANKL‑mediated osteoclastogenesis. Experimental and Therapeutic Medicine, 14, 410-416. https://doi.org/10.3892/etm.2017.4480
MLA
Kim, J., Erkhembaatar, M., Lee, G., Lee, J., Noh, E., Lee, M., Song, H., Lee, C. H., Kwon, K., Kim, M. S., Lee, Y."Peucedanum japonicum Thunb. ethanol extract suppresses RANKL‑mediated osteoclastogenesis". Experimental and Therapeutic Medicine 14.1 (2017): 410-416.
Chicago
Kim, J., Erkhembaatar, M., Lee, G., Lee, J., Noh, E., Lee, M., Song, H., Lee, C. H., Kwon, K., Kim, M. S., Lee, Y."Peucedanum japonicum Thunb. ethanol extract suppresses RANKL‑mediated osteoclastogenesis". Experimental and Therapeutic Medicine 14, no. 1 (2017): 410-416. https://doi.org/10.3892/etm.2017.4480