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Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro

  • Authors:
    • Zhaoxia Zhang
    • Tianyou Wang
    • Ziqin Liu
    • Suoqin Tang
    • Mei Yue
    • Shunqiao Feng
    • Mengze Hu
    • Litian Xuan
    • Yanfei Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology and Oncology, Capital Institute of Pediatrics, Beijing 100020, P.R. China, Department of Hematology and Oncology, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China, Department of Pediatrics, Capital Institute of Pediatrics, Beijing 100020, P.R. China, Department of Pediatrics, People's Liberation Army General Hospital, Beijing 100039, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 35-42
    |
    Published online on: May 23, 2017
       https://doi.org/10.3892/etm.2017.4501
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Abstract

The present study aimed to evaluate the impact of small interfering RNA (siRNA) targeting of the survivin gene in human tumor cells and the effect of decreased survivin expression on the proliferation and apoptosis of tumor cells. Human tumor cell lines (MSA‑MB‑231, SGC‑7901, HeLa, A549, SK‑OV‑3 and Raji, PC‑3) were cultured in vitro and divided into three groups: survivin siRNA‑treated, scrambled negative control siRNA‑treated and an untreated control group. The level of survivin mRNA and protein expression was subsequently determined by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. Cell proliferation was also examined by an MTT assay following transfection and the apoptotic rate of cells was detected by Hoechst and Annexin V/propidium iodide staining. It was observed that relative to the control group, expression of survivin mRNA and protein in the survivin siRNA‑treated group was significantly downregulated. Furthermore, siRNA targeting of survivin lead to the inhibition of tumor cell proliferation, as well as an increase in their apoptotic rate in vitro. These data suggest that survivin may be a potential tumor biomarker and a novel target for the treatment of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Wang T, Liu Z, Tang S, Yue M, Feng S, Hu M, Xuan L and Chen Y: Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. Exp Ther Med 14: 35-42, 2017.
APA
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S. ... Chen, Y. (2017). Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. Experimental and Therapeutic Medicine, 14, 35-42. https://doi.org/10.3892/etm.2017.4501
MLA
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S., Hu, M., Xuan, L., Chen, Y."Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro". Experimental and Therapeutic Medicine 14.1 (2017): 35-42.
Chicago
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S., Hu, M., Xuan, L., Chen, Y."Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro". Experimental and Therapeutic Medicine 14, no. 1 (2017): 35-42. https://doi.org/10.3892/etm.2017.4501
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Wang T, Liu Z, Tang S, Yue M, Feng S, Hu M, Xuan L and Chen Y: Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. Exp Ther Med 14: 35-42, 2017.
APA
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S. ... Chen, Y. (2017). Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. Experimental and Therapeutic Medicine, 14, 35-42. https://doi.org/10.3892/etm.2017.4501
MLA
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S., Hu, M., Xuan, L., Chen, Y."Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro". Experimental and Therapeutic Medicine 14.1 (2017): 35-42.
Chicago
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S., Hu, M., Xuan, L., Chen, Y."Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro". Experimental and Therapeutic Medicine 14, no. 1 (2017): 35-42. https://doi.org/10.3892/etm.2017.4501
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