Open Access

Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro

  • Authors:
    • Zhaoxia Zhang
    • Tianyou Wang
    • Ziqin Liu
    • Suoqin Tang
    • Mei Yue
    • Shunqiao Feng
    • Mengze Hu
    • Litian Xuan
    • Yanfei Chen
  • View Affiliations

  • Published online on: May 23, 2017     https://doi.org/10.3892/etm.2017.4501
  • Pages: 35-42
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to evaluate the impact of small interfering RNA (siRNA) targeting of the survivin gene in human tumor cells and the effect of decreased survivin expression on the proliferation and apoptosis of tumor cells. Human tumor cell lines (MSA‑MB‑231, SGC‑7901, HeLa, A549, SK‑OV‑3 and Raji, PC‑3) were cultured in vitro and divided into three groups: survivin siRNA‑treated, scrambled negative control siRNA‑treated and an untreated control group. The level of survivin mRNA and protein expression was subsequently determined by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. Cell proliferation was also examined by an MTT assay following transfection and the apoptotic rate of cells was detected by Hoechst and Annexin V/propidium iodide staining. It was observed that relative to the control group, expression of survivin mRNA and protein in the survivin siRNA‑treated group was significantly downregulated. Furthermore, siRNA targeting of survivin lead to the inhibition of tumor cell proliferation, as well as an increase in their apoptotic rate in vitro. These data suggest that survivin may be a potential tumor biomarker and a novel target for the treatment of cancer.

Introduction

Identifying effective therapy for the treatment of malignant tumors is a global challenge, and while the development of medical technology has led to an increase in cancer therapies, a treatment strategy of adequate efficacy has not yet been determined. Strategies employing a combination of surgery, radiotherapy, chemotherapy and immunotherapy, as well as clinical-based rehabilitation, have improved the quality of life for cancer patients and increased the 5-year-survival rate (1,2). Combination therapies integrate the research achievements from various specializations, making the treatment more diversified. Combination therapy may exert the advantages and avoid the drawbacks of individual treatments, allowing for the achievement of maximum therapeutic effect. However, due to the presentation of serious complications, side effects (including pain, infection, cardiovascular and gastrointestinal side effects and depression) (3), tumor recurrence and metastasis following therapy, studies investigating alternative therapeutics are warranted. Optimal cancer therapies require the capacity to specifically inhibit tumor cells, while having minimal adverse effects on normal cells. Therefore, the identification of a molecular target that specifically inhibits tumor cell growth is warranted.

Survivin is a member of the inhibitor of apoptosis family of proteins, which collectively has roles in the inhibition of caspase activation and regulation of mitosis. Survivin protein is expressed at high levels in the majority of human tumors and fetal tissue, though is absent in terminally differentiated cells (4). In tumor tissue, survivin promotes the anti-apoptotic activity of tumor cells, while also contributing to tumor angiogenesis and resistance to chemoradiotherapy (57). Specifically, survivin has been found to promote the formation and development of tumors by the following mechanisms: i) interaction with cell division protein kinase 4 leading to acceleration in the S-phase of the cell cycle (8); ii) increasing the expression of human angiopoietin 1, thus promoting the formation of capillary networks and proliferation of endothelial cells (9); iii) overexpression in the G2/M phase of the cell cycle in a cycle-regulated manner and association with microtubules of the mitotic spindle, thus inducing aberrant mitosis (10); and iv) suppressing the release of mitochondrial cytochrome C, thus inhibiting apoptosis and promoting tumor progression (11).

As a commonly upregulated gene in tumor cells, survivin is considered to be a potential cancer biomarker and a novel target for cancer treatment (12). A number of studies have documented the overexpression of survivin in different human tumor types, including leukemia, brain tumors, liver, breast, gastric, lung, colon, prostate, lymph, oesophageal, pancreatic, skin, cervical and ovarian cancer (13). Therefore, studies are aiming to identify novel strategies that target the expression and function of survivin for the treatment of cancer.

RNA interference (RNAi) is an evolution-conserved cell defense mechanism mediated by small interfering RNA (siRNA) (14). Target genes are knocked down by siRNA inhibition of the corresponding mRNA, resulting in suppressed gene expression (15). Compared with conventional antisense strategies, RNAi has demonstrated greater efficacy in the silencing of specific genes by allowing low level target gene expression and subsequent protein function, thus resulting in fewer adverse effects, such as pain, nausea and vomiting, tiredness and sleeping problems, occurring in patients (12). However, rapid degradation of siRNA within the extracellular environment and an inability to traverse cellular membranes means that the delivery of siRNA to its intracellular targets remains a challenge (16).

In the present study, liposomes were used for the intracellular delivery of siRNA against survivin. siRNA liposomal vehicles were successfully constructed and transfected into cell lines (MSA-MB-231, SGC-7901, HeLa, A549, SK-OV-3 and Raji, PC-3) of a number of common human tumor types (breast cancer, gastric carcinoma, cervical carcinoma, lung carcinoma, ovarian carcinoma, lymphoma, prostate carcinoma, respectively). Changes in the level of survivin protein expression and inhibition of tumor cell proliferation and apoptosis were then determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot analysis, the MTT method, Hoechst 33258 staining and Annexin V/PI staining in vitro, in order to determine the efficacy of survivin as a potential gene target in the treatment of cancer.

Materials and methods

Cell lines and culture

Tumor cell lines MDA-MB-231 (human breast carcinoma), SGC-7901 (human gastric carcinoma), HeLa (human cervical carcinoma) and A549 (human lung carcinoma) were obtained from Biomics Biotechnologies Co., Ltd. (Nantong, China). SK-OV-3 (human ovarian carcinoma), Raji (human lymphoma) and PC-3 (human prostate carcinoma) cell lines were purchased from the Cell Banks of Type Culture Collection at the Chinese Academy of Sciences (Beijing, China). MDA-MB-231, SGC-7901 and Raji cells were maintained in RPMI 1640 (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone; GE Healthcare, Logan, UT, USA), and HeLa and A549 cells were maintained in Dulbecco's modified Eagle medium (DMEM; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. SK-OV-3 cells were maintained in McCoy's 5A modified medium (Life Technologies; Thermo Fisher Scientific, Inc.) and PC-3 were maintained in DMEM/ nutrient mixture F12 (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. All cell lines were cultured at 37°C in 5% CO2 for 1–3 days.

siRNA constructs

siRNAs were synthesized using the following sequences: Survivin, forward 5′-GCAUCUCUACAUUCAAGAA-3′ and reverse 5′-UUCUUGAAUGUAGAGAUGC-3′; a scrambled sequence (si-NC), forward 5′-UUCUCCGAACGUGUCACGU-3′ and reverse 5′-ACGUGACACGUUCGGAGAA-3′. si-NC was used as the negative control and was non-homologous to all known human DNA sequences.

Preparation of siRNA-liposomes

siRNA was incorporated into liposomes for intracellular delivery. Disaturated phosphatidylcholine (Avanti Polar Lipids, Alablaster, AL, USA), cholesterol, dioctadecyldimethylammonium chloride and N-palmitoyl-sphingosine-1-succinyl (also known as methoxypolyethylene glycol 2000; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany) were mixed in 100% ethanol at a molar ratio of 25:45:25:2.5 to produce pre-formed vesicles (PFV). siRNA (in a 30% ethanol solution) and PFV's (in a 30% ethanol solution) were warmed to 35–40°C for 10 min, respectively. Subsequently, siRNA was added slowly to the PFVs while the solution was stirred continuously to form siRNA-liposomes. Following the encapsulation, the solution was concentrated by centrifugal filtration (Merck Millipore) and sterilized by filtration using a 0.22-mm Supor membrane filter (Gelman Sciences, Ann Arbor, MI, USA), and the final products were produced. The average diameters of siRNA-survivin-liposomes and siRNA-NC-liposomes were 70.7±29.077 and 64.9±26.128 nm, respectively.

In vitro transfection

Before transfection, the cancer cells at the growth stage were harvested and a cell suspension was prepared (1×105 cells/ml) with the use of DMEM-H medium (Life Technologies; Thermo Fisher Scientific, Inc.). Following this, 1 ml cell suspension was added in each well. The wells were divided into three groups: Survivin siRNA group (survivin siRNA nanoliposome), NC group (NC siRNA nanoliposome) and normal group (without transfection). The cells were plated and grown to 70–80% confluence prior to transfection. Survivin and NC siRNA-liposomes were mixed with Opti-MEM (Life Technologies; Thermo Fisher Scientific, Inc.) and left to stand for 5 min at room temperature. Subsequently, the survivin and NC siRNA-liposomes were mixed with an equal volume of cell suspension and cultured at 37°C in 5% CO2. After 4–6 h, the media were changed to fresh media for growth, as described, and cells were incubated for an additional 48 h at 37°C. A total of 24 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and washed with phosphate buffered saline (PBS) three times, 5 min each time, and stained with Hoechst 33258 (Life Technologies; Thermo Fisher Scientific, Inc.) for 10–20 min. Transfection efficiency was observed using a fluorescence microscope (magnification, ×100; Nikon Corp., Tokyo, Japan).

RNA extraction and RT-qPCR

Total RNA was extracted from the transfected cell lines with RISO™ RNA Isolation Reagent (Biomics Biotechnologies Co., Ltd.), according to the manufacturer's instructions. cDNA was synthesized with a SensiMix™ SYBR-Green One-Step kit (Quantace; Bioline Reagents, Ltd., London, UK) according to the manufacturer's instructions. qPCR was performed using an ABI PRISM real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an endogenous control. PCR primers were as follows: survivin, forward 5′-ACGACCCCATAGAGGAACAT-3′ and reverse, 5′-TCCGCAGTTTCCTCAAATTC-3′; GAP DH, forward 5′-GAAGGTGAAGGTCGGAGTC-3′ and reverse 5′-GAAGATGGTGATGGGATTTC-3′. The RT reaction was performed at 42°C for 30 min. qPCR was then performed under the following conditions: The initial PCR incubation was 95°C for 10 min and the amplification loop (95°C for 20 sec, 58°C for 30 sec and 72°C for 30 sec) was repeated 45 times. Following the reaction, the amplification and melting curves were analyzed. Relative quantification was performed using the comparative 2-ΔΔCq method (17), using GAPDH as a reference gene. All analyses were performed in triplicate.

Western blot analysis

The expression of survivin protein was determined by western blotting. Cells were washed with PBS and placed on ice for 4 min following a 3–5 min incubation in a boiling water bath, then centrifuged at 13,500 × g at 4°C for 15 min. Following centrifugation, the protein pellet was resuspended and the resulting cell protein extract samples were analyzed by 8% SDS-PAGE (15 µl/lane), then transferred to polyvinylidene fluoride membrane filters (EMD Millipore, Billerica, MA, USA). Membranes were incubated at room temperature for 2 h in 5% non-fat dry milk, and rabbit polyclonal anti-survivin antibody (1:500; ab469; Abcam, Cambridge, UK) and mouse anti-β-actin monoclonal antibody (1:400; BM0005; Wuhan Boster Biological Technology, Ltd., Wuhan, China) were added and incubated overnight at 4°C. Following three washes with Tris-buffered saline with Tween, goat anti-rabbit and anti-mouse secondary antibodies (both 1:500; BA1054 and BA1051, respectively; both Wuhan Boster Biological Technology, Ltd.) were added and incubated at room temperature for 2 h. The gray-scale values of the blots were quantified with the computer-based system ImageJ 1.441 (National Institutes of Health, Bethesda, MA, USA). The results were shown as mean values from three replications.

Cell proliferation assay

Growth of the cancer cell lines was evaluated using an MTT assay. The assay was performed at 0, 24, 48, 72 and 96 h post-transfection. Cells were plated in medium supplemented with serum into 96-well plates at a density of 1×104 cells/ml (100 µl). Absorbance was determined following incubation with MTT solution at 37°C without light for 4 h. The supernatant was discarded and 150 µl DMSO was added. Optical density (OD) values were measured at a wavelength of 570 nm with a microplate reader, with normalization to a blank control (dimethyl sulfoxide). The inhibition rate was calculated relative to the OD value in the normal control group.

Hoechst staining

Apoptotic cells were detected by Hoechst 33258 staining. Cells in survivin siRNA, NC and normal groups were fixed with 4% paraformaldehyde for 10 min at room temperature and washed three times with PBS, followed by staining with Hoechst 33258 (Thermo Fisher Scientific, Inc.) for 10–20 min. Apoptotic features were observed under a fluorescence microscope.

Annexin V/propidium iodide (PI) staining

To quantify apoptosis, cells were double-stained with an Annexin V-Fluorescein isothiocyanate Apoptosis Detection kit (Beyotime Institute of Biotechnology, Haimen, China) and PI, according to the manufacturer's protocol. The percentages of cells in different cell cycle phases were then analyzed by FACS Calibur flow cytofluorometry (BD Biosciences, San Jose, CA USA) for the presence of viable (Annexin V and PI), early apoptotic (Annexin V+ and PI) and late apoptotic (Annexin V+ and PI+) cells, as described by a previous method (18).

Statistical analysis

Data are presented as the mean ± standard deviation. Results were statistically analyzed using SPSS 10.0 software (SPSS, Inc., Chicago, IL, USA). A t-test was performed to determine statistical differences between the different groups and P<0.05 was considered to indicate a statistically significant difference.

Results

siRNA transfection suppresses survivin gene expression in human cancer cell lines

Expression of survivin mRNA in the MDA-MB-231, SGC-7901, HeLa, A549, SK-OV-3, Raji and PC-3 cancer cell lines was suppressed following siRNA transfection, as indicated by results of RT-qPCR (Fig. 1A-G, respectively). Among them, cell line SGC-7901 exhibited the greatest sensitivity to survivin siRNA, whereby expression of survivin mRNA significantly decreased to 22% relative to the normal control group (P<0.01, Fig. 1B). Expression of survivin mRNA also significantly decreased compared with the control group in the A549 (36%; P<0.01, Fig. 1D), SK-OV-3 (47%, P<0.01, Fig. 1E) Raji (45%; P<0.05, Fig. 1F) and PC-3 (39%; P<0.05; Fig. 1G) cell lines. Marked decreases in survivin mRNA were observed in MDA-MB-231 (50%, Fig. 1A) and HeLa (65%, Fig. 1C) cells, relative to their respective untreated (normal) control groups. For all cell lines, differences in survivin mRNA levels between the si-NC and normal control groups were not significant.

The level of survivin protein expression in each cell line following survivin siRNA transfection was subsequently determined by western blot analysis. As depicted in Fig. 2, levels of survivin protein in the survivin siRNA groups of all cell lines were significantly (P<0.01) downregulated relative to that in the control groups, particularly for SGC-7901 and PC-3, where the expression was almost completely inhibited. The survivin protein expression levels of the remaining cancer cells were reduced to 40–70% of the level of the normal control group. For all cell lines, differences in survivin protein levels between the si-NC and normal control groups were not significant.

Survivin siRNA inhibits cancer cell proliferation

The inhibitory effects of survivin siRNA in each cell line were analyzed by an MTT assay at 24, 48, 72 and 96 h post-transfection. Inhibition rates are listed in Table I. The data indicates that proliferation of the cancer cell lines was reduced following survivin knockdown by siRNA. At 96 h post-transfection, significant inhibition of cell proliferation was detected in all cell lines relative to the si-NC group (P<0.05).

Table I.

Inhibition rate of survivin siRNA on cancer cell lines.

Table I.

Inhibition rate of survivin siRNA on cancer cell lines.

Inhibition rate (mean ± standard deviation, %)

Cell linesGroups24 h48 h72 h96 h
MDA-MB-231siRNA19.2±4.8a17.5±1.4a16.6±3.7a   9.9±2.2a
si-NC   9.1±5.1   4.1±2.2   4.6±1.9   −1.5±3.7
SGC-7901siRNA   9.0±0.815.5±5.6   5.3±1.8   6.8±4.8a
si-NC   5.2±3.5   8.3±5.0   1.1±4.1   1.1±3.7
HeLasiRNA   7.1±2.218.6±2.2 25.5±4.6a 46.3±1.8a
si-NC   8.6±3.618.3±3.4   5.8±4.233.2±4.3
A549siRNA   4.0±1.0   1.4±2.2 25.4±2.5a 29.4±1.2a
si-NC   2.8±2.4   2.3±2.1   2.9±3.7   3.3±3.1
SK-OV-3siRNA   1.2±2.1   2.8±4.0   −0.9±4.3   7.0±0.9a
si-NC   −1.7±3.3   3.0±3.2   −2.4±3.8   0.4±1.8
RajisiRNA   2.0±0.4   3.0±0.02   1.7±5.0 10.8±4.0a
si-NC   1.4±0.3   0.5±0.7   0.5±3.1   7.4±7.0
PC-3siRNA   1.2±1.8   0.4±1.5   4.7±2.1   5.5±2.7a
si-NC   0.2±2.5   1.0±0.9   1.4±1.9   −0.5±4.1

a P<0.05 vs. si-NC control group. siRNA, small interfering RNA; si-NC, scrambled negative control siRNA.

Survivin knockdown induces apoptosis of cancer cell lines

Hoechst 33258 staining was performed in order to evaluate the levels of cell apoptosis following survivin mRNA knockdown (Fig. 3). Cell nuclei were invariably stained blue by Hoechst 33258, however the nuclei morphology and extent of staining were distinct between normal and apoptotic cells. Normal cells with intact nuclei appeared light blue in color, while apoptotic cells with condensed or fragmented nuclei exhibited bright blue, fragmented nuclei of variable sizes. As depicted in Fig. 3, the nuclei of cancer cell lines transfected with survivin siRNA exhibited granular bright blue fluorescence, reflecting the typical morphological characteristics of apoptosis. These indicators of apoptosis were not observed in the si-NC and normal control groups.

Cells in each group were subsequently double-stained with Annexin V/PI and analyzed with flow cytometry (Fig. 4). The apoptosis rate of MDA-MB-231 and PC-3 cells was significantly increased in the survivin siRNA-transfected groups compared with the apoptosis rate in the si-NC and normal control groups (all P<0.05; Table II). The apoptosis rate was significantly increased in the SGC-7901, HeLa and A549 survivin siRNA-transfected groups compared with the apoptosis rate in the normal control group (all P<0.05; Table II); however, the differences for SK-OV-3 and Raji were not significant.

Table II.

Effect of survivin siRNA on the apoptosis rate of different cancer cells detected by flow cytometry after Annexin-V/propidium iodide staining.

Table II.

Effect of survivin siRNA on the apoptosis rate of different cancer cells detected by flow cytometry after Annexin-V/propidium iodide staining.

Apoptosis rate (mean ± standard deviation, %)

Cell linesSurvivin siRNA groupNC groupNormal group
MDA-MB-231 17.21±2.13a,b   4.29±1.47   3.27±1.32
SGC-7901 19.45±2.58c13.77±2.7112.72±2.83
HeLa   5.37±1.04c   4.45±1.01   2.62±0.91
A549 19.89±3.18c15.29±2.8613.38±2.15
SK-OV-3   4.87±0.84   4.66±0.84   4.53±0.81
Raji   8.98±1.03   8.65±1.02   7.89±0.92
PC-3   7.38±1.56b,d   3.77±1.19   3.72±0.89

{ label (or @symbol) needed for fn[@id='tfn2-etm-0-0-4501'] } Values are presented as the mean ± standard deviation for three separate experiments performed in triplicate.

a P<0.01

b P<0.05 vs. normal group

c P<0.01

d P<0.05 vs. NC group. NC, scrambled negative control siRNA; si, small interfering.

Discussion

Malignant tumors are characterized by uncontrolled and accelerated proliferation of cancer cells and are associated with high morbidity and mortality. In 2012, 14.1 million new cancer cases occurred globally, and cancer resulted 8.2 million deaths (19,20). In the treatment of cancer, combined therapy (21), which includes surgery, chemotherapy, radiotherapy and biological therapy, is generally adopted. Of these, biological therapy is considered to have the greatest efficacy at each stage of tumor development, as well as the least side effects (22). The occurrence and development of tumors is in part due to synergistic and antagonistic interactions between multiple genes, including oncogenes, tumor suppressor genes, apoptosis inhibiting genes and reverse transcriptases. Therefore, along with uncontrolled proliferation of tumor cells, tumorigenesis is also associated with restricted tumor cell apoptosis (23,24).

Survivin is an intracellular protein that belongs to the inhibitor of apoptosis family (5). The protein is considered to be a potential target of cancer therapy, due to its observed overexpression in the majority of malignancies. Therefore, the potential mechanisms of survivin regarding its inhibition of apoptosis have been evaluated (25). As survivin is considered to serve a key regulatory role in cancer progression, methods that suppress survivin expression may promote tumor cell apoptosis and thus have anticancer effects.

RNAi is a post-transcriptional gene silencing mechanism that uses siRNA, and may be used to analyze cancer biology (26). In the present study, RNAi was used to determine the potential role of survivin in human cancer. Specific siRNA against survivin was transfected into a number of human cancer cell lines and its effect on cell proliferation and apoptosis was assessed using RT-qPCR, western blot analysis, MTT assay and Hoechst and Annexin V/PI staining. Using RT-qPCR and western blotting, it was determined that the expression of survivin was suppressed following transfection with survivin siRNA, In addition, data from the MTT assay indicated that proliferation of cancer cells lines was reduced. Subsequent staining assays also demonstrated that siRNA knockdown of survivin led to higher rates of apoptosis in cancer cells. Collectively, these data suggest that survivin may serve a key role in cancer progression.

siRNA technology may be a potential therapeutic method of treating tumors, however the difficulties in delivering siRNA into target cells has limited its use (27). Thus, studies investigating effective delivery systems for siRNA are warranted. A previous study documented the intracellular delivery of siRNA by lipid-based agents and carriers, including atelocollagen, protamine-antibody fusion protein and polyethyleneimine (28). Prior to initiation of the present study, a cationic liposome was optimized and used as an siRNA transfer vehicle. This liposome was chosen due to its ability to efficiently traverse the cell membrane, relatively high stability and biological compatibility.

The distinct distribution of survivin in cancer tissues makes it a suitable target for RNAi-based antitumor therapy, and downregulation of survivin by RNAi has been documented in gastric cancer (29), rectal cancer (30), bladder cancer (31) and lung cancer cells (14). The results of these studies indicated that RNAi-targeting successfully inactivated the anti-apoptotic function of survivin and inhibited tumor growth. Furthermore, expression of survivin in tissues and organs is considered to be an indicator of precancerosis (32), while also being associated with tumor invasion and metastasis (33), sensitivity to chemotherapy (34) and degree of lymphatic metastasis (35). Therefore, survivin is a potential biomarker for the early diagnosis, treatment and prognosis of cancer.

In conclusion, the present study achieved downregulation of survivin in a number of common human cancer cell lines by RNAi. It was observed that siRNA knockdown of survivin inhibited the growth of tumor cells and induced their apoptosis, demonstrating that survivin is a potential gene target in cancer therapy. However, due to differences between in vitro experiments and clinical therapy, the present study was unable to simulate an in vivo environment completely, which made the obtained results biased to some degree. Thus, further studies in animal models are required in the future.

Acknowledgements

The present study was supported by the National High Technology Research and Development Program of China (grant no. 2012AA020804).

References

1 

Sydorak RM and Applebaum H: Soft Tissue TumorsFundamentals of Pediatric Surgery. Mattei P: Springer-Verlag; New York, NY: pp. 755–760. 2011, View Article : Google Scholar

2 

Li W: The new concept of cancer supportive treatment. Electronic Journal of Metabolism and Nutrition of Cancer. 1:2014.

3 

Horiot JC: At last-progress in the assessment of the adverse effects of cancer treatments. Lancet Oncol. 8:568–570. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Barrett PT, Petrides K, Eysenck SB and Eysenck HJ: The Eysenck personality questionnaire: An examination of the factorial similarity of PE, N and L across 34 countries. Personality Individual Differences. 25:805–819. 1998. View Article : Google Scholar

5 

Ambrosini G, Adida C and Altieri DC: A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med. 3:917–921. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Zaffaroni N and Daidone MG: Survivin expression and resistance to anticancer treatments: Perspectives for new therapeutic interventions. Drug Resistance Updates. 5:65–72. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Kong LJ, Zhao J and Fan WW: Survivin and Tumor Angiogenesis. Zhong Liu Xue Za Zhi. 15:950–952. 2009.(In Chinese).

8 

Suzuki A, Hayashida M, Ito T, Kawano H, Nakano T, Miura M, Akahane K and Shiraki K: Survivin initiates cell cycle entry by the competitive interaction with Cdk4/p16 (INK4a) and Cdk2/cyclin E complex activation. Oncogene. 19:3225–3234. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Mesri M, Morales-Ruiz M, Ackermann EJ, Bennett CF, Pober JS, Sessa WC and Altieri DC: Suppression of vascular endothelial growth factor-mediated endothelial cell protection by survivin targeting. Am J Pathol. 158:1757–1765. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC and Altieri DC: Control of apoptosis and mitotic spindle checkpoint by survivin. Nature. 396:580–584. 1998. View Article : Google Scholar : PubMed/NCBI

11 

Kayaselcuk F, Nursal T, Polat A, Noyan T, Yildirim S, Tarim A and Seydaoglu G: Expresion of survivin, Bcl-2, P53 and bax in breast carcioma and ductal intraepithelial neoplasia (DIN 1a). J Exp Clin Cancer Res. 23:105–112. 2004.PubMed/NCBI

12 

Ryan BM, O'Donovan N and Duffy MJ: Survivin: A new target for anti-cancer therapy. Cancer Treat Rev. 35:553–562. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Altieri DC: Survivin, versatile modulation of cell division and apoptosis in cancer. Oncogene. 22:8581–8589. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Chen XQ, Yang S, Li ZY, Lu HS, Kang MQ and Lin TY: Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells. Mol Med Rep. 5:917–922. 2012.PubMed/NCBI

15 

Baker B, Eldrup A, Manoharan M, et al: RNA-induced silencing complexes; RNA interference; gene expression inhibition US patent 10/700,884. October 4–2003, issued February 17. 2005

16 

Aliabadi H Montazeri, Landry B, Mahdipoor P and Uludag H: Induction of apoptosis by survivin silencing through siRNA delivery in a human breast cancer cell line. Mol Pharm. 8:1821–1830. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Mirandola P, Sponzilli I, Gobbi G, Marmiroli S, Rinaldi L, Binazzi R, Piccari GG, Ramazzotti G, Gaboardi GC, Cocco L and Vitale M: Anticancer agents sensitize osteosarcoma cells to TNF-related apoptosis-inducing ligand downmodulating IAP family proteins. Int J Oncol. 28:127–133. 2006.PubMed/NCBI

19 

Stewart B and Wild C: World cancer report 2014. International Agency for Research on Cancer; Lyon: 2014

20 

Organization WH: ES Fact sheet 310: Top causes of death. Centro De Prensa. 2014.

21 

Arnold LD, Ji QS, Buck E, Haley JD and Mulvihill MJ: Combination cancer therapy U.S. Patent 8,575,164. 2013

22 

Yang Y and Huang S: Discuss the strengths and weaknesses between tumour biotherapy and chemoradiotherapy. Medical Information. 24:3503–3504. 2011.

23 

Hengartner MO: The biochemistry of apoptosis. Nature. 407:770–776. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Reed JC: Dysregulation of apoptosis in cancer. J Clin Oncol. 17:2941–2953. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Chiou SK, Jones MK and Tarnawski AS: Survivin-an anti-apoptosis protein: Its biological roles and implications for cancer and beyond. Med Sci Monit. 9:PI25–PI29. 2003.PubMed/NCBI

26 

Merritt WM, Bar-Eli M and Sood AK: The dicey role of Dicer: Implications for RNAi therapy. Cancer Res. 70:2571–2574. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Semple SC, Akinc A, Chen J, Sandhu AP, Mui BL, Cho CK, Sah DW, Stebbing D, Crosley EJ, Yaworski E, et al: Rational design of cationic lipids for siRNA delivery. Nat Biotechnol. 28:172–176. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Medarova Z, Pham W, Farrar C, Petkova V and Moore A: In vivo imaging of siRNA delivery and silencing in tumors. Nat Med. 13:372–377. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Wenying Z, Zhaoning J, Zhimin Y, Dongyun C and Lili S: Survivin siRNA inhibits gastric cancer in nude mice. Cell Biochem Biophys. 62:337–341. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Rödel F, Hoffmann J, Distel L, Herrmann M, Noisternig T, Papadopoulos T, Sauer R and Rödel C: Survivin as a radioresistance factor, and prognostic and therapeutic target for radiotherapy in rectal cancer. Cancer Res. 65:4881–4887. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Ning S, Fuessel S, Kotzsch M, Kraemer K, Kappler M, Schmidt U, Taubert H, Wirth MP and Meye A: siRNA-mediated down-regulation of survivin inhibits bladder cancer cell growth. Int J Oncol. 25:1065–1071. 2004.PubMed/NCBI

32 

Endoh A, Asanuma K, Moriai R, Yamada M, Koyanagi Y, Sato T, Yagihasi A, Nakamura M, Kobayashi D and Watanabe N: Expression of survivin mRNA in CD34-positive cells. Clin Chim Acta. 306:149–151. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Lee GH, Joo YE, Koh YS, Chung IJ, Park YK, Lee JH, Kim HS, Choi SK, Rew JS, Park CS and Kim SJ: Expression of survivin in gastric cancer and its relationship with tumor angiogenesis. Eur J Gastroenterol Hepatol. 18:957–963. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Smith SD, Wheeler MA, Plescia J, Colberg JW, Weiss RM and Altieri DC: Urine detection of survivin and diagnosis of bladder cancer. JAMA. 285:324–328. 2001. View Article : Google Scholar : PubMed/NCBI

35 

Yao X, Liu F, Qi X, Wu B, Yin HL, Ma HH, Shi QL, Zhou XJ and Li JS: Expression of survivin in human gastric adenocarcinomas: Correlation with proliferation and apoptosis. Zhonghua Wai Ke Za Zhi. 42:145–148. 2004.(In Chinese). PubMed/NCBI

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July-2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang Z, Wang T, Liu Z, Tang S, Yue M, Feng S, Hu M, Xuan L and Chen Y: Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. Exp Ther Med 14: 35-42, 2017
APA
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S. ... Chen, Y. (2017). Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro. Experimental and Therapeutic Medicine, 14, 35-42. https://doi.org/10.3892/etm.2017.4501
MLA
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S., Hu, M., Xuan, L., Chen, Y."Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro". Experimental and Therapeutic Medicine 14.1 (2017): 35-42.
Chicago
Zhang, Z., Wang, T., Liu, Z., Tang, S., Yue, M., Feng, S., Hu, M., Xuan, L., Chen, Y."Small interfering RNA targeting of the survivin gene inhibits human tumor cell growth in vitro". Experimental and Therapeutic Medicine 14, no. 1 (2017): 35-42. https://doi.org/10.3892/etm.2017.4501