Open Access

Antimicrobial peptide LL-37 promotes the viability and invasion of skin squamous cell carcinoma by upregulating YB-1

  • Authors:
    • Wei Wang
    • Yan Zheng
    • Jinjing Jia
    • Changji Li
    • Qiqi Duan
    • Ruilian Li
    • Xin Wang
    • Yongping Shao
    • Caifeng Chen
    • Huling Yan
  • View Affiliations

  • Published online on: June 6, 2017     https://doi.org/10.3892/etm.2017.4546
  • Pages: 499-506
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Antimicrobial peptide LL-37 serves a function in the host defense against microbial invasion, and also regulates cell proliferation, immune activity and angiogenesis. Previous studies have reported that LL‑37 participates in the development of numerous tumour types, such as ovarian cancer, lung cancer, melanoma and breast cancer. However, the function of LL‑37 in the development of skin squamous cell carcinoma (SCC) has not yet been fully elucidated. The aim of the current study was to investigate how LL‑37 promotes the expression of Y‑box binding protein 1 (YB‑1) in SCC. Short interfering RNA (siRNA) was used to inhibit the expression of YB‑1, and in vitro MTT and Transwell migration assays were used to evaluate the effect of reduced YB‑1 on the viability and invasion of A431 cells. A431 cells were stimulated with LL‑37, and quantitative polymerase chain reaction, immunofluorescence and western blot analyses were used to detect changes in YB‑1 expression. Mitogen‑activated protein kinase kinase, mitogen‑activated protein kinase and nuclear factor (NF)‑κB signaling pathway inhibitors were also used to evaluate the mechanism of LL‑37‑induced YB‑1 protein expression. It was found that YB‑1 expression was increased in SCC tissue compared with normal tissue. Inhibiting YB‑1 expression using siRNA significantly reduced the viability and suppressed the invasion of tumour cells (P<0.05 for both). LL‑37 treatment at 0.05 µg/ml for 24 or 48 h significantly promoted YB‑1 protein expression (P<0.05), and this was dependent on the NF‑κB signaling pathway. In conclusion, the current study demonstrated that by upregulating the expression of YB‑1, LL‑37 can promote the occurrence and development of SCC, and this process involves the NF-κB signaling pathway.
View Figures
View References

Related Articles

Journal Cover

July-2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang W, Zheng Y, Jia J, Li C, Duan Q, Li R, Wang X, Shao Y, Chen C, Yan H, Yan H, et al: Antimicrobial peptide LL-37 promotes the viability and invasion of skin squamous cell carcinoma by upregulating YB-1. Exp Ther Med 14: 499-506, 2017
APA
Wang, W., Zheng, Y., Jia, J., Li, C., Duan, Q., Li, R. ... Yan, H. (2017). Antimicrobial peptide LL-37 promotes the viability and invasion of skin squamous cell carcinoma by upregulating YB-1. Experimental and Therapeutic Medicine, 14, 499-506. https://doi.org/10.3892/etm.2017.4546
MLA
Wang, W., Zheng, Y., Jia, J., Li, C., Duan, Q., Li, R., Wang, X., Shao, Y., Chen, C., Yan, H."Antimicrobial peptide LL-37 promotes the viability and invasion of skin squamous cell carcinoma by upregulating YB-1". Experimental and Therapeutic Medicine 14.1 (2017): 499-506.
Chicago
Wang, W., Zheng, Y., Jia, J., Li, C., Duan, Q., Li, R., Wang, X., Shao, Y., Chen, C., Yan, H."Antimicrobial peptide LL-37 promotes the viability and invasion of skin squamous cell carcinoma by upregulating YB-1". Experimental and Therapeutic Medicine 14, no. 1 (2017): 499-506. https://doi.org/10.3892/etm.2017.4546