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Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration

  • Authors:
    • Eun‑Jung Yang
    • Sa‑Ik Bang
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    Affiliations: Department of Plastic Surgery, Cheil General Hospital and Women's Healthcare Center, College of Medicine, Dankook University, Seoul 06351, Republic of Korea, Department of Plastic Surgery, Samsung Medical Center, School of Medicine, Sungkyunkwan University, Seoul 06351, Republic of Korea
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 723-729
    |
    Published online on: June 7, 2017
       https://doi.org/10.3892/etm.2017.4558
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Abstract

Adipose stem cell-conditioned medium may promote human dermal fibroblast (HDF) proliferation and migration by activating paracrine peptides during the re‑epithelization phase of wound healing. Human antimicrobial peptide LL‑37 is upregulated in the skin epithelium as part of the normal response to injury. The effects of conditioned medium (CM) from LL‑37 treated adipose stem cells (ASCs) on cutaneous wound healing, including the mediation of fibroblast migration, remain to be elucidated, therefore the aim of the present study was to determine how ASCs would react to an LL‑37‑rich microenvironment and if CM from LL‑37 treated ASCs may influence the migration of HDFs. The present study conducted migration assays with HDFs treated with CM from LL‑37 treated ASCs. Expression of CXC chemokine receptor 4 (CXCR4), which controls the recruitment of HDFs, was analyzed at the mRNA and protein levels. To further characterize the stimulatory effects of LL‑37 on ASCs, the expression of stromal cell‑derived factor‑1α (SDF‑1α), a CXC chemokine, was investigated. CM from LL‑37‑treated ASCs induced migration of HDFs in a time‑ and dose‑dependent manner, with a maximum difference in migration observed 24 h following stimulation with LL‑37 at a concentration of 10 µg/ml. The HDF migration and the expression of CXCR4 in fibroblasts was markedly increased upon treatment with CM from LL‑37‑treated ASCs compared with CM from untreated ASCs. SDF‑1α expression was markedly increased in CM from LL‑37 treated ASCs. It was additionally observed that SDF‑1α blockade significantly reduced HDF migration. These findings suggest the feasibility of CM from LL‑37‑treated ASCs as a potential therapeutic for human dermal fibroblast migration.
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Copy and paste a formatted citation
Spandidos Publications style
Yang EJ and Bang SI: Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration. Exp Ther Med 14: 723-729, 2017.
APA
Yang, E., & Bang, S. (2017). Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration. Experimental and Therapeutic Medicine, 14, 723-729. https://doi.org/10.3892/etm.2017.4558
MLA
Yang, E., Bang, S."Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration". Experimental and Therapeutic Medicine 14.1 (2017): 723-729.
Chicago
Yang, E., Bang, S."Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration". Experimental and Therapeutic Medicine 14, no. 1 (2017): 723-729. https://doi.org/10.3892/etm.2017.4558
Copy and paste a formatted citation
x
Spandidos Publications style
Yang EJ and Bang SI: Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration. Exp Ther Med 14: 723-729, 2017.
APA
Yang, E., & Bang, S. (2017). Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration. Experimental and Therapeutic Medicine, 14, 723-729. https://doi.org/10.3892/etm.2017.4558
MLA
Yang, E., Bang, S."Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration". Experimental and Therapeutic Medicine 14.1 (2017): 723-729.
Chicago
Yang, E., Bang, S."Effects of conditioned medium from LL‑37 treated adipose stem cells on human fibroblast migration". Experimental and Therapeutic Medicine 14, no. 1 (2017): 723-729. https://doi.org/10.3892/etm.2017.4558
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