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Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice

  • Authors:
    • Wen Guan
    • Xiaolan Yu
    • Jingjing Li
    • Qing Deng
    • Yang Zhang
    • Jing Gao
    • Peng Xia
    • Yunsheng Yuan
    • Jin Gao
    • Liang Zhou
    • Wei Han
    • Yan Yu
  • View Affiliations / Copyright

    Affiliations: Shanghai Municipality Key Laboratory of Veterinary Biotechnology, Department of Animal Sciences, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, P.R. China, Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, P.R. China
    Copyright: © Guan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1001-1008
    |
    Published online on: June 12, 2017
       https://doi.org/10.3892/etm.2017.4578
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Abstract

Although hair loss or alopecia is a common disease, its exact mechanisms are not yet well understood. The present study investigated the hypothesis that the homeostatic regulation of genes during hair regeneration may participate in hair loss, based on the cyclicity of hair growth. A cluster of such genes was identified by an expression gene‑array from the dorsal skin in a depilated mouse model, and CXCL4 was identified as a significantly regulated gene during the hair regeneration process. To elucidate the function of CXCL4 in hair growth, CXCL4 activity was blocked by the admi­nistration of an anti‑CXCL4 monoclonal antibody (mAb). Histomorphometric analysis indicated that anti‑CXCL4 mAb induced an earlier anagen phase and delayed hair follicle regression, in contrast with that in the control group. Moreover, CXCL4 mAb upregulated the transcription levels of several hair growth‑related genes, including Lef1, Wnt10b, Bmp4 and Bmp2. In addition, CXCL4 mAb increased the levels of the proliferation‑related protein PCNA and Bcl‑2 during the anagen phase, while it reduced the expression of pro‑apoptotic protein Bax and cleaved caspase‑3 during the catagen phase. These findings reveal that CXCL4 plays an important role in hair growth, and that blockade of CXCL4 activity promotes hair growth.
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1 

Müller-Röver S, Handjiski B, van der Veen C, Eichmüller S, Foitzik K, McKay IA, Stenn KS and Paus R: A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol. 117:3–15. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Schneider MR, Schmidt-Ullrich R and Paus R: The hair follicle as a dynamic miniorgan. Curr Biol. 19:R132–R142. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Wang TL, Zhou C, Shen YW, Wang XY, Ding XL, Tian S, Liu Y, Peng GH, Xue SQ, Zhou JE, et al: Prevalence of androgenetic alopecia in China: A community-based study in six cities. Br J Dermatol. 162:843–847. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Cotsarelis G and Millar SE: Towards a molecular understanding of hair loss and its treatment. Trends Mol Med. 7:293–301. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Zhang NN, Park DK and Park HJ: Hair growth-promoting activity of hot water extract of Thuja orientalis. BMC Complement Altern Med. 13:92013. View Article : Google Scholar : PubMed/NCBI

6 

Miller MD and Krangel MS: Biology and biochemistry of the chemokines: A family of chemotactic and inflammatory cytokines. Crit Rev Immunol. 12:17–46. 1992.PubMed/NCBI

7 

Deutsch E, Johnson SA and Seegers WH: Differentiation of certain platelet factors related to blood coagulation. Circ Res. 3:110–115. 1955. View Article : Google Scholar : PubMed/NCBI

8 

Rendu F and Brohard-Bohn B: The platelet release reaction: Granules' constituents, secretion and functions. Platelets. 12:261–273. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Kasper B and Petersen F: Molecular pathways of platelet factor 4/CXCL4 signaling. Eur J Cell Biol. 90:521–526. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Scheuerer B, Ernst M, Dürrbaum-Landmann I, Fleischer J, Grage-Griebenow E, Brandt E, Flad HD and Petersen F: The CXC-chemokine platelet factor 4 promotes monocyte survival and induces monocyte differentiation into macrophages. Blood. 95:1158–1166. 2000.PubMed/NCBI

11 

Han ZC, Lu M, Li J, Defard M, Boval B, Schlegel N and Caen JP: Platelet factor 4 and other CXC chemokines support the survival of normal hematopoietic cells and reduce the chemosensitivity of cells to cytotoxic agents. Blood. 89:2328–2335. 1997.PubMed/NCBI

12 

Gupta SK and Singh JP: Inhibition of endothelial cell proliferation by platelet factor-4 involves a unique action on S phase progression. J Cell Biol. 127:1121–1127. 1994. View Article : Google Scholar : PubMed/NCBI

13 

Luster AD, Greenberg SM and Leder P: The IP-10 chemokine binds to a specific cell surface heparan sulfate site shared with platelet factor 4 and inhibits endothelial cell proliferation. J Exp Med. 182:219–231. 1995. View Article : Google Scholar : PubMed/NCBI

14 

Maione TE, Gray GS, Petro J, Hunt AJ, Donner AL, Bauer SI, Carson HF and Sharpe RJ: Inhibition of angiogenesis by recombinant human platelet factor-4 and related peptides. Science. 247:77–79. 1990. View Article : Google Scholar : PubMed/NCBI

15 

Watson JB, Getzler SB and Mosher DF: Platelet factor 4 modulates the mitogenic activity of basic fibroblast growth factor. J Clin Invest. 94:261–268. 1994. View Article : Google Scholar : PubMed/NCBI

16 

Gao J, Gao J, Qian L, Wang X, Wu M, Zhang Y, Ye H, Zhu S, Yu Y and Han W: Activation of p38-MAPK by CXCL4/CXCR3 axis contributes to p53-dependent intestinal apoptosis initiated by 5-fluorouracil. Cancer Biol Ther. 15:982–991. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Paus R, Stenn KS and Link RE: Telogen skin contains an inhibitor of hair growth. Br J Dermatol. 122:777–784. 1990. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Plonka PM, Michalczyk D, Popik M, Handjiski B, Slominski A and Paus R: Splenic eumelanin differs from hair eumelanin in C57BL/6 mice. Acta Biochim Pol. 52:433–441. 2005.PubMed/NCBI

20 

Datta K, Singh AT, Mukherjee A, Bhat B, Ramesh B and Burman AC: Eclipta alba extract with potential for hair growth promoting activity. J Ethnopharmacol. 124:450–456. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Huelsken J, Vogel R, Erdmann B, Cotsarelis G and Birchmeier W: beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell. 105:533–545. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Van Mater D, Kolligs FT, Dlugosz AA and Fearon ER: Transient activation of beta -catenin signaling in cutaneous keratinocytes is sufficient to trigger the active growth phase of the hair cycle in mice. Genes Dev. 17:1219–1224. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Mundy G, Gutierrez G, Garrett R, Gallwitz W, Rossini G, Christiansen C and Langenberg A: Proteasome inhibitors stimulate both bone formation and hair growth by similar mechanisms. Ann N Y Acad Sci. 1117:298–301. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Dong L, Hao H, Xia L, Liu J, Ti D, Tong C, Hou Q, Han Q, Zhao Y, Liu H, et al: Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth. Sci Rep. 4:54322014. View Article : Google Scholar : PubMed/NCBI

25 

Gentilini G, Kirschbaum NE, Augustine JA, Aster RH and Visentin GP: Inhibition of human umbilical vein endothelial cell proliferation by the CXC chemokine, platelet factor 4 (PF4), is associated with impaired downregulation of p21(Cip1/WAF1). Blood. 93:25–33. 1999.PubMed/NCBI

26 

Woller G, Brandt E, Mittelstädt J, Rybakowski C and Petersen F: Platelet factor 4/CXCL4-stimulated human monocytes induce apoptosis in endothelial cells by the release of oxygen radicals. J Leukoc Biol. 83:936–945. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Soma T, Fujiwara S, Shirakata Y, Hashimoto K and Kishimoto J: Hair-inducing ability of human dermal papilla cells cultured under Wnt/β-catenin signalling activation. Exp Dermatol. 21:307–309. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Fuchs E and Raghavan S: Getting under the skin of epidermal morphogenesis. Nat Rev Genet. 3:199–209. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Li YH, Zhang K, Yang K, Ye JX, Xing YZ, Guo HY, Deng F, Lian XH and Yang T: Adenovirus-mediated Wnt10b overexpression induces hair follicle regeneration. J Invest Dermatol. 133:42–48. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Andl T, Reddy ST, Gaddapara T and Millar SE: WNT signals are required for the initiation of hair follicle development. Dev Cell. 2:643–653. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Gat U, DasGupta R, Degenstein L and Fuchs E: De Novo hair follicle morphogenesis and hair tumors in mice expressing a truncated beta-catenin in skin. Cell. 95:605–614. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Jiang S, Zhao L, Teklemariam T and Hantash BM: Small cutaneous wounds induce telogen to anagen transition of murine hair follicle stem cells. J Dermatol Sci. 60:143–150. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Adams JM and Cory S: The Bcl-2 protein family: Arbiters of cell survival. Science. 281:1322–1326. 1998. View Article : Google Scholar : PubMed/NCBI

34 

Lindner G, Botchkarev VA, Botchkareva NV, Ling G, van der Veen C and Paus R: Analysis of apoptosis during hair follicle regression (catagen). Am J Pathol. 151:1601–1617. 1997.PubMed/NCBI

35 

Botchkareva NV, Ahluwalia G and Shander D: Apoptosis in the hair follicle. J Invest Dermatol. 126:258–264. 2006. View Article : Google Scholar : PubMed/NCBI

36 

Sabbagh L, Kaech SM, Bourbonnière M, Woo M, Cohen LY, Haddad EK, Labrecque N, Ahmed R and Sékaly RP: The selective increase in caspase-3 expression in effector but not memory T cells allows susceptibility to apoptosis. J Immunol. 173:5425–5433. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guan W, Yu X, Li J, Deng Q, Zhang Y, Gao J, Xia P, Yuan Y, Gao J, Zhou L, Zhou L, et al: Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice. Exp Ther Med 14: 1001-1008, 2017.
APA
Guan, W., Yu, X., Li, J., Deng, Q., Zhang, Y., Gao, J. ... Yu, Y. (2017). Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice. Experimental and Therapeutic Medicine, 14, 1001-1008. https://doi.org/10.3892/etm.2017.4578
MLA
Guan, W., Yu, X., Li, J., Deng, Q., Zhang, Y., Gao, J., Xia, P., Yuan, Y., Gao, J., Zhou, L., Han, W., Yu, Y."Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice". Experimental and Therapeutic Medicine 14.2 (2017): 1001-1008.
Chicago
Guan, W., Yu, X., Li, J., Deng, Q., Zhang, Y., Gao, J., Xia, P., Yuan, Y., Gao, J., Zhou, L., Han, W., Yu, Y."Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1001-1008. https://doi.org/10.3892/etm.2017.4578
Copy and paste a formatted citation
x
Spandidos Publications style
Guan W, Yu X, Li J, Deng Q, Zhang Y, Gao J, Xia P, Yuan Y, Gao J, Zhou L, Zhou L, et al: Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice. Exp Ther Med 14: 1001-1008, 2017.
APA
Guan, W., Yu, X., Li, J., Deng, Q., Zhang, Y., Gao, J. ... Yu, Y. (2017). Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice. Experimental and Therapeutic Medicine, 14, 1001-1008. https://doi.org/10.3892/etm.2017.4578
MLA
Guan, W., Yu, X., Li, J., Deng, Q., Zhang, Y., Gao, J., Xia, P., Yuan, Y., Gao, J., Zhou, L., Han, W., Yu, Y."Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice". Experimental and Therapeutic Medicine 14.2 (2017): 1001-1008.
Chicago
Guan, W., Yu, X., Li, J., Deng, Q., Zhang, Y., Gao, J., Xia, P., Yuan, Y., Gao, J., Zhou, L., Han, W., Yu, Y."Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1001-1008. https://doi.org/10.3892/etm.2017.4578
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