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Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice

  • Authors:
    • Xiao Liu
    • Deguo Wang
    • Zhengzheng Zhang
    • Fenghui Zhu
    • Aiming Yao
    • Jiwei Tian
    • Dengshun Miao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China, Department of Obstetrics and Gynecology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Emergency Center, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1141-1145
    |
    Published online on: June 12, 2017
       https://doi.org/10.3892/etm.2017.4584
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Abstract

This study aims to investigate the function and related mechanism of P27 gene in intervertebral disc (IVD) degeneration of mice. X-ray, immunohistochemical staining, and alkaline phosphatase (ALP) histochemical staining were used to analyze the phenotypic difference of the intervertebral discs of 4-week-old mice with P27 gene knockout (P27‑/-) and wild‑type (WT) mice in the same brood. Protein in the intervertebral disc was extracted and western blot analysis was employed to detect the changes in the expression of related molecules in the Shh-signal pathways, including Shh, Patched, Smoothened and Gli2. As a result, the ALP histochemical staining revealed that the ALP‑positive area of mice in the P27-/- group was obviously increased compared to the 4-week-old mice of the same brood in the WT group. In addition, the Col-Ⅰ immunohistochemical staining showed that the Col-Ⅰ‑positive area of mice in the P27-/- group was significantly increased compared to mice in the WT group. Furthermore, Smo‑positive cell rate of mice in the P27-/- group was apparently increased compared to mice in the WT group. Western blot analysis revealed that in terms of changes of protein expression levels of Shh, Patched, Smoothened and Gli2 in the intervertebral disc, protein expression levels of Shh, Patched, Smoothened and Gli2 of mice in the P27-/- group were significantly increased compared to those of mice in the WT group. The results show that P27 deficiency activates the expression of Shh-signal pathway and promotes the proliferation of osteoblast, thus, playing a role in promoting IVD degeneration, which provides a scientific and reliable experimental basis for the treatment of the IVD degeneration‑related diseases in clinical practice.
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1 

DeLucca JF, Cortes DH, Jacobs NT, Vresilovic EJ, Duncan RL and Elliott DM: Human cartilage endplate permeability varies with degeneration and intervertebral disc site. J Biomech. 49:550–557. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Chen HT, Huang AB, He YL, Bian J and Li HJ: Wnt11 overexpression promote adipose-derived stem cells differentiating to the nucleus pulposus-like phenotype. Eur Rev Med Pharmacol Sci. 21:1462–1470. 2017.PubMed/NCBI

3 

Cui YZ, Yang XH, Liu PF, Wang B and Chen WJ: Preliminary study on diagnosis of lumbar disc degeneration with magnetic resonance T1p, T2 mapping and DWI quantitative detection technologies. Eur Rev Med Pharmacol Sci. 20:3344–3350. 2016.PubMed/NCBI

4 

Ma T, Guo CJ, Zhao X, Wu L, Sun SX and Jin QH: The effect of curcumin on NF-κB expression in rat with lumbar intervertebral disc degeneration. Eur Rev Med Pharmacol Sci. 19:1305–1314. 2015.PubMed/NCBI

5 

Liu C, Fei HD, Sun ZY and Tian JW: Bioinformatic analysis of the microarray gene expression profile in degenerative intervertebral disc cells exposed to TNF-α. Eur Rev Med Pharmacol Sci. 19:3332–3339. 2015.PubMed/NCBI

6 

Baranto A, Ekström L, Holm S, Hellström M, Hansson HA and Swärd L: Vertebral fractures and separations of endplates after traumatic loading of adolescent porcine spines with experimentally-induced disc degeneration. Clin Biomech (Bristol, Avon). 20:1046–1054. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Lundin O, Ekström L, Hellström M, Holm S and Swärd L: Exposure of the porcine spine to mechanical compression: Differences in injury pattern between adolescents and adults. Eur Spine J. 9:466–471. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Mackiewicz Z, Salo J, Konttinen YT, Holm A Kaigle, Indahl A, Pajarinen J and Holm S: Receptor activator of nuclear factor kappa B ligand in an experimental intervertebral disc degeneration. Clin Exp Rheumatol. 27:299–306. 2009.PubMed/NCBI

9 

Shimura-Miura H, Hattori N, Kang D, Miyako K, Nakabeppu Y and Mizuno Y: Increased 8-oxo-dGTPase in the mitochondria of substantia nigral neurons in Parkinsons disease. Ann Neurol. 46:920–924. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Bach FC, Zhang Y, Miranda-Bedate A, Verdonschot LC, Bergknut N, Creemers LB, Ito K, Sakai D, Chan D, Meij BP and Tryfonidou MA: Increased caveolin-1 in intervertebral disc degeneration facilitates repair. Arthritis Res Ther. 18:592016. View Article : Google Scholar : PubMed/NCBI

11 

Thoreson O, Baranto A, Ekström L, Holm S, Hellström M and Swärd L: The immediate effect of repeated loading on the compressive strength of young porcine lumbar spine. Knee Surg Sports Traumatol Arthrosc. 18:694–701. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Arpinar VE, Rand SD, Klein AP, Maiman DJ and Muftuler LT: Changes in perfusion and diffusion in the endplate regions of degenerating intervertebral discs: A DCE-MRI study. Eur Spine J. 24:2458–2467. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Wang B, Wang D, Yan T and Yuan H: MiR-138-5p promotes TNF-α-induced apoptosis in human intervertebral disc degeneration by targeting SIRT1 through PTEN/PI3K/Akt signaling. Exp Cell Res. 345:199–205. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Jang TW, Ahn YS, Byun J, Lee JI, Kim KH, Kim Y, Song HS, Lee CG, Kwon YJ, Yoon JH and Jeong K: Lumbar intervertebral disc degeneration and related factors in Korean firefighters. BMJ Open. 6:e0115872016. View Article : Google Scholar : PubMed/NCBI

15 

Qin C, Zhang B, Zhang L, Zhang Z, Wang L, Tang L, Li S, Yang Y, Yang F, Zhang P and Yang B: MyD88-dependent Toll-like receptor 4 signal pathway in intervertebral disc degeneration. Exp Ther Med. 12:611–618. 2016.PubMed/NCBI

16 

Yang H, Yuan C, Wu C, Qian J, Shi Q, Li X, Zhu X and Zou J: The role of TGF-β1/Smad2/3 pathway in platelet-rich plasma in retarding intervertebral disc degeneration. J Cell Mol Med. 20:1542–1549. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Nicole W, Tellegen AR, Niklas B, Creemers LB, Jeannette W, Christian F, Benz K, Grinwis GCM, Tryfonidou MA and Meij BP: Inflammatory profiles in canine intervertebral disc degeneration. BMC Vet Res. 12:1–12. 2016.PubMed/NCBI

18 

Zhang F, Zhao X, Shen H and Zhang C: Molecular mechanisms of cell death in intervertebral disc degeneration (Review). Int J Mol Med. 37:1439–1448. 2016.PubMed/NCBI

19 

Lv FJ, Peng Y, Lim FL, Sun Y, Lv M, Zhou L, Wang H, Zheng Z, Cheung KM and Leung VY: Matrix metalloproteinase 12 is an indicator of intervertebral disc degeneration co-expressed with fibrotic markers. Osteoarthritis Cartilage. 24:1826–1836. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Luo Y, Zhang L, Wang WY, Hu QF, Song HP and Zhang YZ: The inhibitory effect of salmon calcitonin on intervertebral disc degeneration in an ovariectomized rat model. Eur Spine J. 24:1691–1701. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Choi KS, Cohn MJ and Harfe BD: Identification of nucleus pulposus precursor cells and notochordal remnants in the mouse: Implications for disk degeneration and chordoma formation. Dev Dyn. 237:3953–3958. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Dahia CL, Mahoney E and Wylie C: Shh signaling from the nucleus pulposus is required for the postnatal growth and differentiation of the mouse intervertebral disc. PLoS One. 7:e359442012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Wang D, Zhang Z, Zhu F, Yao A, Tian J and Miao D: Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice. Exp Ther Med 14: 1141-1145, 2017.
APA
Liu, X., Wang, D., Zhang, Z., Zhu, F., Yao, A., Tian, J., & Miao, D. (2017). Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice. Experimental and Therapeutic Medicine, 14, 1141-1145. https://doi.org/10.3892/etm.2017.4584
MLA
Liu, X., Wang, D., Zhang, Z., Zhu, F., Yao, A., Tian, J., Miao, D."Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice". Experimental and Therapeutic Medicine 14.2 (2017): 1141-1145.
Chicago
Liu, X., Wang, D., Zhang, Z., Zhu, F., Yao, A., Tian, J., Miao, D."Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1141-1145. https://doi.org/10.3892/etm.2017.4584
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Wang D, Zhang Z, Zhu F, Yao A, Tian J and Miao D: Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice. Exp Ther Med 14: 1141-1145, 2017.
APA
Liu, X., Wang, D., Zhang, Z., Zhu, F., Yao, A., Tian, J., & Miao, D. (2017). Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice. Experimental and Therapeutic Medicine, 14, 1141-1145. https://doi.org/10.3892/etm.2017.4584
MLA
Liu, X., Wang, D., Zhang, Z., Zhu, F., Yao, A., Tian, J., Miao, D."Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice". Experimental and Therapeutic Medicine 14.2 (2017): 1141-1145.
Chicago
Liu, X., Wang, D., Zhang, Z., Zhu, F., Yao, A., Tian, J., Miao, D."Research on the function and related mechanism of P27 gene in the intervertebral disc degeneration of mice". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1141-1145. https://doi.org/10.3892/etm.2017.4584
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