MicroRNA-375 targets PAX6 and inhibits the viability, migration and invasion of human breast cancer MCF-7 cells Retraction in /10.3892/etm.2021.10603

Zou,Qiongyan; Yi,Wenjun; Huang,Jianghai; Fu,Fenfen; Chen,Gannong; Zhong,Dewu

doi:10.3892/etm.2017.4593

MicroRNA-375 targets PAX6 and inhibits the viability, migration and invasion of human breast cancer MCF-7 cells

Retraction in: /10.3892/etm.2021.10603

Authors:
- Qiongyan Zou
- Wenjun Yi
- Jianghai Huang
- Fenfen Fu
- Gannong Chen
- Dewu Zhong
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Affiliations: Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
Published online on: June 13, 2017 https://doi.org/10.3892/etm.2017.4593
Pages: 1198-1204

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Abstract

MicroRNAs (miRs) are a type of small non-coding RNA that serve crucial roles in the development and progression of breast cancer. However, the exact role and underlying molecular mechanism of miR‑375 in mediating the growth and metastasis of breast cancer remains unknown. In the present study, reverse transcription‑quantitative polymerase chain reaction and western blot analysis were conducted to examine RNA and protein expression. A luciferase reporter assay was performed to determine the association between miR‑375 and paired box 6 (PAX6). The results of the current study indicate that the expression of miR‑375 was reduced in breast cancer tissues compared with matched adjacent normal tissues. Transfection with miR‑375 mimics led to a significant increase in levels of miR‑375 in human breast cancer Michigan Cancer Foundation (MCF)‑7 cells (P<0.05). The increase in miR‑375 expression caused a significant decrease in the viability, migration and invasion of MCF‑7 cells (P<0.05), accompanied by a reduced expression of matrix metalloproteinase (MMP) 2 and MMP9 proteins. Luciferase reporter assay identified PAX6 as a novel target of miR‑375 and miR‑375 in turn, negatively regulated the protein expression of PAX6 in MCF‑7 cells. By contrast, overexpression of PAX6 led to a significant increase in MCF‑7 cell viability (P<0.01) but did not affect the migration and invasion of MCF‑7 cells, suggesting that the inhibitory effect of miR‑375 on MCF‑7 cell viability may be occurring, in part, via the direct targeting of PAX6.

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