Open Access

MicroRNA-130a is highly expressed in the esophageal mucosa of achalasia patients

  • Authors:
    • Hiroyuki Shoji
    • Hajime Isomoto
    • Akira Yoshida
    • Haruo Ikeda
    • Hitomi Minami
    • Tsutomu Kanda
    • Shigetoshi Urabe
    • Kayoko Matsushima
    • Fuminao Takeshima
    • Kazuhiko Nakao
    • Haruhiro Inoue
  • View Affiliations

  • Published online on: June 13, 2017     https://doi.org/10.3892/etm.2017.4598
  • Pages: 898-904
  • Copyright: © Shoji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Esophageal achalasia is considered as a risk factor of esophageal cancer. The etiologies of esophageal achalasia remain unknown. Peroral endoscopic myotomy (POEM) has recently been established as a minimally invasive method with high curability. The aims of the present study were to identify the microRNAs (miRs) specific to esophageal achalasia, to determine their potential target genes and to assess their alteration following POEM. RNA was extracted from biopsy samples from middle esophageal mucosa and analyzed using a microarray. Differentially expressed miRs in achalasia patients compared with control samples were identified and analyzed using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Correlations between specific miR expression levels and the patients' clinical background were also investigated. In addition, alterations of selected miR expression levels before and after POEM were analyzed. The results of RT‑qPCR analysis demonstrated that the miR‑130a expression levels were significantly higher in patients with achalasia (P<0.0001). In addition, miR‑130a expression was significantly correlated with male sex and smoking history in patients with achalasia. However, no significant change in miR‑130a expression was observed between before and after POEM. In conclusion, miR‑130a is highly expressed in the esophageal mucosa of patients with achalasia and may be a biomarker of esophageal achalasia.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Shoji H, Isomoto H, Yoshida A, Ikeda H, Minami H, Kanda T, Urabe S, Matsushima K, Takeshima F, Nakao K, Nakao K, et al: MicroRNA-130a is highly expressed in the esophageal mucosa of achalasia patients. Exp Ther Med 14: 898-904, 2017
APA
Shoji, H., Isomoto, H., Yoshida, A., Ikeda, H., Minami, H., Kanda, T. ... Inoue, H. (2017). MicroRNA-130a is highly expressed in the esophageal mucosa of achalasia patients. Experimental and Therapeutic Medicine, 14, 898-904. https://doi.org/10.3892/etm.2017.4598
MLA
Shoji, H., Isomoto, H., Yoshida, A., Ikeda, H., Minami, H., Kanda, T., Urabe, S., Matsushima, K., Takeshima, F., Nakao, K., Inoue, H."MicroRNA-130a is highly expressed in the esophageal mucosa of achalasia patients". Experimental and Therapeutic Medicine 14.2 (2017): 898-904.
Chicago
Shoji, H., Isomoto, H., Yoshida, A., Ikeda, H., Minami, H., Kanda, T., Urabe, S., Matsushima, K., Takeshima, F., Nakao, K., Inoue, H."MicroRNA-130a is highly expressed in the esophageal mucosa of achalasia patients". Experimental and Therapeutic Medicine 14, no. 2 (2017): 898-904. https://doi.org/10.3892/etm.2017.4598