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Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress

  • Authors:
    • Hui Zhao
    • Bingyu Liang
    • Linjie Yu
    • Yun Xu
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210008, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1086-1094
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    Published online on: June 14, 2017
       https://doi.org/10.3892/etm.2017.4601
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Abstract

Jieyu chufan (JYCF) is a well-known Chinese traditional medicine used for depression; however, the molecular mechanism underlying its anti‑depressant action has remained elusive. In the present study, the anti‑depressant effects of JYCF and the potential mechanisms were investigated in a mouse model. Five groups of 12 C57BL/6 mice each were used in the study, including a normal control group (NC group), a model control group (MC group) and three groups, which received different doses of JYCF (1.25, 2.5 and 5 g/kg) orally for 21 days (JYCF groups). The MC group and the three JYCF groups were subjected to 3 weeks of unpredictable chronic mild stress (UCMS) to induce depression‑like behavior. All groups were subjected to a sucrose consumption test along with a forced swimming test to confirm depression‑like behavior, an open‑field test and an elevated plus maze test to confirm anxiety‑like behavior, and a Morris water maze test to evaluate spatial learning and memory. In addition, synaptic density in the hippocampus was evaluated and western blot and immunostaining were used to analyze hippocampal expression of postsynaptic density protein‑95 (PSD95), synaptophysin (Syn), cyclic adenosine monophosphate response element binding protein (CREB), brain‑derived neurotrophic factor (BDNF), Akt and glycogen synthase kinase (GSK)‑3β as well as their phosphorylated (p) versions. The results showed that JYCF (2.5 and 5 g/kg) alleviated depressive‑like behaviors and increased synaptic density in UCMS mice. Moreover, JYCF upregulated the expression of PSD95, Syn and BDNF and increased phosphorylated Akt, CREB and GSK‑3β in the hippocampus. These results suggested that JYCF exerts an anti‑depressant‑like activity in UCMS‑induced mice, which is likely to be mediated by reversing the stress‑induced disruption of BDNF and GSK-3β activity.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao H, Liang B, Yu L and Xu Y: Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress. Exp Ther Med 14: 1086-1094, 2017.
APA
Zhao, H., Liang, B., Yu, L., & Xu, Y. (2017). Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress. Experimental and Therapeutic Medicine, 14, 1086-1094. https://doi.org/10.3892/etm.2017.4601
MLA
Zhao, H., Liang, B., Yu, L., Xu, Y."Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress". Experimental and Therapeutic Medicine 14.2 (2017): 1086-1094.
Chicago
Zhao, H., Liang, B., Yu, L., Xu, Y."Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1086-1094. https://doi.org/10.3892/etm.2017.4601
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao H, Liang B, Yu L and Xu Y: Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress. Exp Ther Med 14: 1086-1094, 2017.
APA
Zhao, H., Liang, B., Yu, L., & Xu, Y. (2017). Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress. Experimental and Therapeutic Medicine, 14, 1086-1094. https://doi.org/10.3892/etm.2017.4601
MLA
Zhao, H., Liang, B., Yu, L., Xu, Y."Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress". Experimental and Therapeutic Medicine 14.2 (2017): 1086-1094.
Chicago
Zhao, H., Liang, B., Yu, L., Xu, Y."Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1086-1094. https://doi.org/10.3892/etm.2017.4601
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