Open Access

miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma

  • Authors:
    • Chengliang Cui
    • Xiaoyu Shi
  • View Affiliations

  • Published online on: June 16, 2017     https://doi.org/10.3892/etm.2017.4624
  • Pages: 1045-1050
  • Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) play crucial roles in cancer development and progression. The purposes of this study were to investigate the role of miR-187 in osteosarcoma and clarify the regulation of MAPK12 by miR‑187. Quantitative polymerase chain reaction was used to examine miR‑187 expression in osteosarcoma tissues and cell lines. The clinicopathological significance of miR‑187 downregulation was further analyzed. Transwell migration and invasion assays were performed. A luciferase reporter assay was conducted to confirm the target gene of miR‑187, and the results were validated in cervical cancer tissues and cell lines. MiR‑187 was significantly decreased in clinical tissues and osteosarcoma cell lines. The low miR‑187 level was significantly correlated with stage, node metastasis, and deep stromal invasion. Upregulation of miR‑187 suppressed cell migration and invasion in vitro. MAPK12 was verified as a direct target of miR‑187, which was further confirmed by the inverse expression of miR‑187 and MAPK12 in patients' specimens. The newly identified miR-187/MAPK12 pathway provides an insight into osteosarcoma metastasis and may represent a novel therapeutic target.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Cui C and Cui C: miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma. Exp Ther Med 14: 1045-1050, 2017
APA
Cui, C., & Cui, C. (2017). miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma. Experimental and Therapeutic Medicine, 14, 1045-1050. https://doi.org/10.3892/etm.2017.4624
MLA
Cui, C., Shi, X."miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma". Experimental and Therapeutic Medicine 14.2 (2017): 1045-1050.
Chicago
Cui, C., Shi, X."miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1045-1050. https://doi.org/10.3892/etm.2017.4624