Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Komohara,Yoshihiro; Kawauchi,Ryuta; Makiyama,Erika; Mikami,Kazuki; Horlad,Hasita; Fujiwara,Yukio; Kida,Tetsuya; Takeya,Motohiro; Niidome,Takuro

doi:10.3892/etm.2017.4640

Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Authors:
- Yoshihiro Komohara
- Ryuta Kawauchi
- Erika Makiyama
- Kazuki Mikami
- Hasita Horlad
- Yukio Fujiwara
- Tetsuya Kida
- Motohiro Takeya
- Takuro Niidome
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Affiliations: Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860‑8556, Japan, Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, Kumamoto 860‑8555, Japan, Magnesium Research Center, Kumamoto University, Kumamoto 860‑8555, Japan
Published online on: June 21, 2017 https://doi.org/10.3892/etm.2017.4640
Pages: 1640-1646

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Abstract

Previous studies have indicated pro‑tumor functions of macrophages in tumor progression in different types of malignant tumors. The detailed mechanisms of cell‑cell interaction between macrophages and tumor cells have been investigated by means of in vitro co‑culture experiments. The present study developed magnetite nanoparticles modified with gelatin that are specifically engulfed by macrophages and investigated methods to deplete these macrophages in co‑culture experiments using a magnet. T98G glioma cell line and human monocyte‑derived macrophages were mixed and co‑cultured for 2 days. The T98G cells were isolated by depletion of the macrophages using the magnetite nanoparticles. mRNA expression of a number of pro‑tumor molecules in the isolated T98G cells, with or without co‑culture with macrophages, was then evaluated. The mRNA expression levels of chemokine (CC motif) ligand 2, interleukin‑6 and macrophage‑colony stimulating factor receptor (M‑CSFR) were significantly upregulated in T98G cells by co‑culture with macrophages (P<0.01). M‑CSFR protein expression was also increased by co‑culture with macrophages. The conditioned medium of co‑cultured cells increased M‑CSFR expression in T98G cells. Magnetite nanoparticles may be a novel tool not only for investigating the unique activation status of tumor cells in co‑culture conditions, but also for targeting pro-tumor macrophages in tumor tissues.

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