Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2017 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation

Retraction in: /10.3892/etm.2021.9977
  • Authors:
    • Xiang‑Dong Yao
    • Ping Li
    • Ji‑Sheng Wang
  • View Affiliations / Copyright

    Affiliations: Ear, Nose and Throat Department, Xinxiang Central Hospital, Xinxiang, Henan 453000, P.R. China
  • Pages: 1699-1705
    |
    Published online on: June 27, 2017
       https://doi.org/10.3892/etm.2017.4685
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to screen and analyze the differential expression spectrum of microRNA (miRNA) between laryngeal cancer tissue and surrounding normal laryngeal mucosa in order to provide an indication for further study to determine the role of miRNA in the initiation and development of laryngeal cancer. A total of 42 pairs of specimens of laryngeal carcinoma tissues and adjacent normal laryngeal mucosa were collected. A total of 10 pairs of specimens were randomly selected for miRNA microarray gene chip analysis, and the remaining 32 pairs of specimens were used for reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) verification to identify miRNA that were differentially expressed in laryngeal cancer tissues. Methylthiazolyldiphenyl‑tetrazolium bromide and clone formation assays were utilized to elucidate the physiological relevance of the miRNA miR‑125a‑5p on the proliferation of laryngeal cancer human epithelial type 2 (Hep2) cells. Results demonstrated that the expression levels of six miRNA were significantly downregulated in laryngeal carcinoma tissue, as identified by gene chip analysis and RT‑qPCR (P<0.05). The six miRNA included let‑7f‑5p, miR‑10a‑5p, miR‑125a‑5p, miR‑144‑3p, miR‑195‑5p and miR‑203. Compared with the control group, the proliferative ability of laryngeal cancer Hep2 cells was inhibited in a transfected miR‑125a‑mimics group. In contrast, proliferation was promoted in a transfected miR‑125a‑inhibitor group. In conclusion, the results of gene chip analysis were consistent with that of RT‑qPCR. Results demonstrated that miRNA in laryngeal cancer and normal laryngeal mucosa exhibited evident differential expression, which may contribute to the laryngeal cancer incidence and invasion. miR‑125a was able to inhibit the proliferation of Hep2 laryngeal cancer cells and, therefore, may serve as a novel target for laryngeal cancer biological therapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Lu ZM: Differential microRNA expression profiling in laryngeal cancer and study of mir-125a on the proliferation of laryngeal cancer cell line. Southern Med Univ Master's Degree2013

2 

Jaseviciene L, Gurevicius R, Obelenis V, Cicenas S and Juozulynas A: Trends in laryngeal cancer incidence in ithuania: A future perspective. Int J Occup Med Environ Health. 17:473–477. 2004.PubMed/NCBI

3 

Lu ST, Wei KR, Yu BH, et al: Incidence trend analysis of laryngeal carcinoma. Xian Dai Zhong Liu Yi Xue. 12:158–160. 2004.(In Chinese).

4 

Hoffman HT, Porter K, Karnell LH, Cooper JS, Weber RS, Langer CJ, Ang KK, Gay G, Stewart A and Robinson RA: Laryngeal cancer in the United States: Changes in demographics, patterns of care and survival. Laryngoscope. 116 Suppl 111:S1–S13. 2006. View Article : Google Scholar

5 

Martin DA, James O, Allen SL and John EN: Clinical oncology. Peking: Scientific Publishing Agency; pp. 18012001

6 

Hashibe M, Boffetta P, Zaridze D, Shangina O, Szeszenia-Dabrowska N, Mates D, Fabiánová E, Rudnai P and Brennan P: Contribution of tobacco and alcohol to the high rates of squamous cell carcinoma of the supraglottis and glottis in central Europe. Am J Epidemiol. 165:814–820. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Potter CS, Wang Z, Silva KA, Kennedy VE, Stearns TM, Burzenski L, Shultz LD, Hogenesch H and Sundberg JP: Chronic proliferative dermatitis in sharpin null mice: Development of an autoinflammatory disease in the absence of B and T lymphocytes and IL4/IL13 signaling. PLoS One. 9:e856662014. View Article : Google Scholar : PubMed/NCBI

8 

Li P and Wang X: Relationship between Myc gene family and laryngeal cancer. Shi Yong Zhong Liu Xue Za Zhi. 16:67–69. 2002.(In Chinese).

9 

Pietruszewska W, Durko M and Kobos J: Alterations of cell cycle regulating proteins: Rb, p21 and p16 in laryngeal cancer. Otolaryngol Pol. 61:951–957. 2007.(In Polish). View Article : Google Scholar : PubMed/NCBI

10 

Croce CM and Calin GA: MiRNAs, cancer and stem cell division. Cell. 122:6–7. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Esquela-Kerscher A and Slack FJ: Oncomirs: MicroRNAs with a role in cancer. Nat Rev Cancer. 6:259–269. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Wang Z, Potter CS, Sundberg JP and Hogenesch H: SHARPIN is a key regulator of immune and inflammatory responses. J Cell Mol Med. 16:2271–2279. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Ramdas L, Giri U, Ashorn CL, Coombes KR, El-Naggar A, Ang KK and Story MD: MiRNA expression profiles in head and neck squamous cell carcinoma and adjacent normal tissue. Head Neck. 31:642–654. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Schmittgen TD, Lee EJ, Jiang J, Sarkar A, Yang L, Elton TS and Chen C: Real-time PCR quantification of precursor and mature microRNA. Methods. 44:31–38. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, et al: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33:e1792005. View Article : Google Scholar : PubMed/NCBI

18 

Zhang C, Zeng X, Li Z, Wang Z and Li S: Immunoglobulin A nephropathy: Current progress and future directions. Transl Res. 166:134–144. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Jemal A, Siegel R, Ward E, Murray T, Xu J, Smigal C and Thun MJ: Cancer statistics, 2006. CA Cancer J Clin. 56:106–130. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Huang Q, Yu GP, Mccormick SA, Mo J, Datta B, Mahimkar M, Lazarus P, Schäffer AA, Desper R and Schantz SP: Genetic differences detected by comparative genomic hybridization in head and neck squamous cell carcinomas from different tumor sites: Construction of oncogenetic trees for tumor progression. Genes Chromosomes Cancer. 34:224–233. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Li YD, Gowhere Ali and Hong SL: Relationship between single nucleotide polymorphism in genome carcinoma tissue and tumor metastasis. Aca J Sec Mil Med Univ. 32:713–716. 2011. View Article : Google Scholar

22 

Wang J: The application of gene chip technology on head and neck neoplasm. Otol Forei Med Sci. 29:382–385. 2005.

23 

Hui AB, Lenarduzzi M, Krushel T, Waldron L, Pintilie M, Shi W, Perez-Ordonez B, Jurisica I, O'Sullivan B, Waldron J, et al: Comprehensive microRNA profiling for head and neck squamous cell carcinomas. Clin Cancer Res. 16:1129–1139. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Zhong Q, Fang JG, Huang ZG, et al: A preliminary study of miRNA expression in laryngeal squamous cell carcinoma. Chin J Cancer Prev Control. 17:1073–1076. 2010.(In Chinese).

25 

Wang P, Fu T, Wang XR, et al: Preliminary study on the differential expression of miRNA and normal mucosa in laryngeal squamous cell carcinoma by microarray analysis. Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 24:535–538. 2010.(In Chinese). PubMed/NCBI

26 

Wu YB, Shen ZS, Yu X, et al: Research advances of microRNAs related to laryngeal carcinoma. Ji Chu Yi Xue Yu Lin Chuang. 32:583–586. 2012.(In Chinese).

27 

Yang J, Zhang Q, Dong JQ, Chang XH and He XJ: Overexpression of high mobility group A2 and its correlation with microRNA let-7 falimy in serous ovarian cancers. Beijing Da Xue Xue Bao. 44:749–754. 2012.(In Chinese). PubMed/NCBI

28 

Chen FY and Chen QY: Research progress in the correlation between MicroRNA let-7 and cancer. Yi Xue Zong Shu. 17:1797–1800. 2011.(In Chinese).

29 

Tran N, Mclean T, Zhang X, Zhao CJ, Thomson JM, O'Brien C and Rose B: MicroRNA expression profiles in head and neck cancer cell lines. Biochem Biophys Res Commun. 358:12–17. 2007. View Article : Google Scholar : PubMed/NCBI

30 

He JF, Luo YM, Wan XH and Jiang D: Biogenesis of miRNA-195 and its role in biogenesis, the cell cycle and apoptosis. J Biochem Mol Toxicol. 25:404–408. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Zhang S, Li YJ, Zhang C, et al: Study on the role of K562 in apoptosis induced by norcantharidin in miRNA. Zhong Guo Bing Li Sheng Li Za Zhi. 27:499–503. 2011.(In Chinese).

32 

Bi Q, Tang S, Xia L, Du R, Fan R, Gao L, Jin J, Liang S, Chen Z, Xu G, et al: Ectopic expression of miR-125a inhibits the proliferation and metastasis of hepatocellular carcinoma by targeting MMP11 and VEGF. PLoS One. 7:e401692012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yao XD, Li P and Wang JS: MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977. Exp Ther Med 14: 1699-1705, 2017.
APA
Yao, X., Li, P., & Wang, J. (2017). MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977. Experimental and Therapeutic Medicine, 14, 1699-1705. https://doi.org/10.3892/etm.2017.4685
MLA
Yao, X., Li, P., Wang, J."MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977". Experimental and Therapeutic Medicine 14.2 (2017): 1699-1705.
Chicago
Yao, X., Li, P., Wang, J."MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1699-1705. https://doi.org/10.3892/etm.2017.4685
Copy and paste a formatted citation
x
Spandidos Publications style
Yao XD, Li P and Wang JS: MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977. Exp Ther Med 14: 1699-1705, 2017.
APA
Yao, X., Li, P., & Wang, J. (2017). MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977. Experimental and Therapeutic Medicine, 14, 1699-1705. https://doi.org/10.3892/etm.2017.4685
MLA
Yao, X., Li, P., Wang, J."MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977". Experimental and Therapeutic Medicine 14.2 (2017): 1699-1705.
Chicago
Yao, X., Li, P., Wang, J."MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation Retraction in /10.3892/etm.2021.9977". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1699-1705. https://doi.org/10.3892/etm.2017.4685
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team