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Article

Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation

  • Authors:
    • Rui Zhang
    • Yunhong Li
    • Xiaolin Hou
    • Zhenhua Miao
    • Yin Wang
  • View Affiliations / Copyright

    Affiliations: Ningxia Key Laboratory of Cerebrocranial Diseases, Basic Medical School of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Neurology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
  • Pages: 1832-1836
    |
    Published online on: June 27, 2017
       https://doi.org/10.3892/etm.2017.4691
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Abstract

Matrine (MT) is the primary active alkaloid separated from members of the Sophora genus. Previous studies have reported that MT has anti‑inflammatory effects in the central nervous system (CNS). However, the underlying molecular mechanism of the neuroprotective effect of MT remains unclear, particularly the role of heat shock protein 60 (HSP60). Microglia are macrophages in the CNS that serve an essential role in the innate immune system by producing various proinflammatory and neurotoxic factors. In addition, HSP60 is released by activated microglia causing an autoimmune response. The present study aimed to investigate whether MT could inhibit the activation of microglia via suppressing the HSP60 signaling pathway. The results demonstrated that the expression and release of HSP60 in LPS‑activated BV2 microglial cells was significantly decreased by MT treatment. Extracellular HSP60 is a ligand of toll like receptor 4 (TLR‑4); thus, it was hypothesized that secreted HSP60 could bind to TLR‑4 on microglia and activate the TLR‑4 signaling pathway. As expected, western blotting and ELISA results revealed that MT significantly inhibited the LPS‑induced increase in TLR‑4, myeloid differentiation primary response protein MyD88, caspase‑3 and tumor necrosis factor‑α. In conclusion, the results of the present study provide a novel direction for the prevention and treatment of neurodegenerative diseases characterized by microglial activation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang R, Li Y, Hou X, Miao Z and Wang Y: Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation. Exp Ther Med 14: 1832-1836, 2017.
APA
Zhang, R., Li, Y., Hou, X., Miao, Z., & Wang, Y. (2017). Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation. Experimental and Therapeutic Medicine, 14, 1832-1836. https://doi.org/10.3892/etm.2017.4691
MLA
Zhang, R., Li, Y., Hou, X., Miao, Z., Wang, Y."Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation". Experimental and Therapeutic Medicine 14.2 (2017): 1832-1836.
Chicago
Zhang, R., Li, Y., Hou, X., Miao, Z., Wang, Y."Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1832-1836. https://doi.org/10.3892/etm.2017.4691
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang R, Li Y, Hou X, Miao Z and Wang Y: Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation. Exp Ther Med 14: 1832-1836, 2017.
APA
Zhang, R., Li, Y., Hou, X., Miao, Z., & Wang, Y. (2017). Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation. Experimental and Therapeutic Medicine, 14, 1832-1836. https://doi.org/10.3892/etm.2017.4691
MLA
Zhang, R., Li, Y., Hou, X., Miao, Z., Wang, Y."Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation". Experimental and Therapeutic Medicine 14.2 (2017): 1832-1836.
Chicago
Zhang, R., Li, Y., Hou, X., Miao, Z., Wang, Y."Neuroprotective effect of heat shock protein 60 on matrine‑suppressed microglial activation". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1832-1836. https://doi.org/10.3892/etm.2017.4691
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