Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin‑induced mouse model of asthma

Nie,Hanxiang; Wang,Ailing; He,Qing; Yang,Qiaoyu; Liu,Linlin; Zhang,Guqin; Huang,Yi; Ding,Xuhong; Yu,Hongying; Hu,Suping

doi:10.3892/etm.2017.4699

Phenotypic switch in lung interstitial macrophage polarization in an ovalbumin‑induced mouse model of asthma

Authors:
- Hanxiang Nie
- Ailing Wang
- Qing He
- Qiaoyu Yang
- Linlin Liu
- Guqin Zhang
- Yi Huang
- Xuhong Ding
- Hongying Yu
- Suping Hu
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Affiliations: Department of Respiratory Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Nursing Department, Wuhan University School of Health Sciences, Wuhan, Hubei 430071, P.R. China, Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: June 28, 2017 https://doi.org/10.3892/etm.2017.4699
Pages: 1284-1292
Copyright: © Nie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Macrophage phenotype and function varies according to their polarized state, which in turn is dependent on microenvironmental stimuli. Under normal physiological conditions, lung interstitial macrophages that express interleukin (IL)‑10 are considered to serve regulatory roles in the prevention of allergic reactions in the airways. However, the phenotypic profile of lung interstitial macrophages during the pathophysiology of asthma remains unknown. In the current study, the phenotypic characteristics of lung interstitial macrophages were investigated in an ovalbumin (OVA)‑induced mouse model of asthma. The patterns of surface markers chemokine ligand and interleukin, and the metabolic enzyme activity of lung interstitial macrophages were investigated using flow cytometry analysis, reverse transcription‑quantitative polymerase chain reaction, western blot analysis, and ELISA. It was observed that lung interstitial macrophages derived from OVA‑induced asthmatic mice expressed phenotypic markers associated with alternatively activated macrophages (M2), including cluster of differentiation‑206, transglutaminase 2, arginase (Arg) 1 and chemokine ligand (CCL)17/CCL22/CCL24 secretion. The M2 macrophages also exhibited increased levels of Arg1 activity and reduced levels of IL‑10 expression, relative to macrophages derived from control mice. However, when evaluating the expression of markers associated with classically activated (M1) macrophages, namely inducible nitric oxide synthase and IL‑12, it was observed that levels of M1 markers in the interstitial macrophages from asthmatic mice did not differ significantly to those in controls. Collectively, these data suggest that lung interstitial macrophages undergo a phenotypic switch from a regulatory macrophage phenotype under normal conditions to an alternative activation state in OVA‑induced asthmatic mice.

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