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Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis

  • Authors:
    • Yunliang Wang
    • Lijuan Liu
    • Yi Guo
    • Tangyou Mao
    • Rui Shi
    • Junxiang Li
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China, Gastroenterology Department of Traditional Chinese Medicine, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1327-1336
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    Published online on: June 28, 2017
       https://doi.org/10.3892/etm.2017.4701
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Abstract

The effects of indigo naturalis (IN), which is a traditional Chinese herbal formulation, have been clinically demonstrated in treating refractory ulcerative colitis (UC). The present study aimed to verify the effects and mechanisms of IN in experimental UC rats. A total of 48 male Sprague‑Dawley rats were randomly divided into six groups: Chow, model, high‑dose IN, medium‑dose IN, low‑dose IN and mesalazine (a bowel‑specific aminosalicylate drug) groups. The models were administered 3.5% dextran sodium sulphate solution for 7 days. The treatment groups were administered IN or mesalazine and then sacrificed and sampled on day 8. Disease activity index (DAI), histological damage score (HDS) and myeloperoxidase (MPO) activity were used to evaluate the severity of UC. Colon and serum cytokines were detected using liquid‑phase chip technology and the expression of occludin protein in colonic mucosa was assessed by immunohistochemistry and western blot analysis. The results indicated that the oral administration of IN may reduce DAI, HDS and MPO activity. IN also reduced the expression of inflammatory cytokines and increased the expression of colonic mucosal repair‑related cytokines and occludin protein. These results highlight the potential of IN as a therapeutic agent for treating UC through its action of inflammation control and colonic mucosal damage repair.
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Spandidos Publications style
Wang Y, Liu L, Guo Y, Mao T, Shi R and Li J: Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis. Exp Ther Med 14: 1327-1336, 2017.
APA
Wang, Y., Liu, L., Guo, Y., Mao, T., Shi, R., & Li, J. (2017). Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis. Experimental and Therapeutic Medicine, 14, 1327-1336. https://doi.org/10.3892/etm.2017.4701
MLA
Wang, Y., Liu, L., Guo, Y., Mao, T., Shi, R., Li, J."Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis". Experimental and Therapeutic Medicine 14.2 (2017): 1327-1336.
Chicago
Wang, Y., Liu, L., Guo, Y., Mao, T., Shi, R., Li, J."Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1327-1336. https://doi.org/10.3892/etm.2017.4701
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Spandidos Publications style
Wang Y, Liu L, Guo Y, Mao T, Shi R and Li J: Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis. Exp Ther Med 14: 1327-1336, 2017.
APA
Wang, Y., Liu, L., Guo, Y., Mao, T., Shi, R., & Li, J. (2017). Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis. Experimental and Therapeutic Medicine, 14, 1327-1336. https://doi.org/10.3892/etm.2017.4701
MLA
Wang, Y., Liu, L., Guo, Y., Mao, T., Shi, R., Li, J."Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis". Experimental and Therapeutic Medicine 14.2 (2017): 1327-1336.
Chicago
Wang, Y., Liu, L., Guo, Y., Mao, T., Shi, R., Li, J."Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate‑induced ulcerative colitis". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1327-1336. https://doi.org/10.3892/etm.2017.4701
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