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Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain

  • Authors:
    • Ying Wang
    • Wei Wang
    • Xilong Wang
    • Yangyun Wang
    • Jihong Wang
    • Qiang Fu
    • Guowei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China, Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1337-1342
    |
    Published online on: June 28, 2017
       https://doi.org/10.3892/etm.2017.4705
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Abstract

The implantation of a suburethral sling is an important treatment for stress urinary incontinence (SUI). However, the slings used current have a number of inherent limitations, such as tissue rejection and infection. The present study investigated the potential of engineering sling tissue in vitro using adipose‑derived stem cells (ADSCs). The ADSCs were obtained from Sprague‑Dawley rats and were characterized in vitro. The ADSCs were seeded on polyglycolic acid (PGA) fibers that formed a scaffold with a shape mimicking a sling complex. The results demonstrated that following in vitro culture for 12 weeks under static strain, neo‑sling tissue could be generated using ADSCs. With increasing culture time, the engineered neo‑sling tissue exhibited a significant improvement in biomechanical properties, including maximal load and Young's modulus (P<0.05), and the tissue and collagen structures matured. Furthermore, differentiated ADSCs cultured under static strain were maintained their myoblast phenotype within the PGA scaffolds. These results indicate that ADSCs may serve as a novel cell source for tissue sling engineering and could improve treatment for patients with SUI.
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1 

Abrams P, Cardozo L, Fall M, Griffiths D, Rosier P, Ulmsten U, van Kerrebroeck P, Victor A and Wein A: The standardisation of terminology of lower urinary tract function: Report from the Standardisation Sub-committee of the International Continence Society. Am J Obstet Gynecol. 187:116–126. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Klausner AP and Vapnek JM: Urinary incontinence in the geriatric population. Mt Sinai J Med. 70:54–61. 2003.PubMed/NCBI

3 

Rutner AB, Levine SR and Schmaelzle JF: Processed porcine small intestine submucosa as a graft material for pubovaginal slings: Durability and results. Urology. 62:805–809. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Hung MJ, Liu FS, Shen PS, Chen GD, Lin LY and Ho ES: Analysis of two sling procedures using polypropylene mesh for treatment of stress urinary incontinence. Int J Gynaecol Obstet. 84:133–141. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Stav K, Dwyer PL, Rosamilia A, Schierlitz L, Lim YN, Chao F, De Souza A, Thomas E, Murray C, Conway C and Lee J: Repeat synthetic mid urethral sling procedure for women with recurrent stress urinary incontinence. J Urol. 183:241–246. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Walter AJ, Morse AN, Leslie KO, Zobitz ME, Hentz JG and Cornella JL: Changes in tensile strength of cadaveric human fascia lata after implantation in a rabbit vagina model. J Urol. 169:1907–1910. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Kim IG, Piao S, Hong SH, Kim SW, Hwang TK, Oh SH, Lee JH and Lee JY: The effect of a bioactive tissue-engineered sling in a rat of stress incontinence model. J Biomed Mater Res A. 100:286–292. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Zou XH, Zhi YL, Chen X, Jin HM, Wang LL, Jiang YZ, Yin Z and Ouyang HW: Mesenchymal stem cell seeded knitted silk sling for the treatment of stress urinary incontinence. Biomaterials. 31:4872–4879. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Lee JY, Cannon TW, Pruchnic R, Fraser MO, Huard J and Chancellor MB: The effects of periurethral muscle-derived stem cell injection on leak point pressure in a rat model of stress urinary incontinence. Int Urogynecol J Pelvic Floor Dysfunct. 14:31–37. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Zhao W, Zhang C, Jin C, Zhang Z, Kong D, Xu W and Xiu Y: Periurethral injection of autologous adipose-derived stem cells with controlled-release nerve growth factor for the treatment of stress urinary incontinence in a rat model. Eur Urol. 59:155–163. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Li GY, Zhou F, Gong YQ, Cui WS, Yuan YM, Song WD, Xin H, Liu T, Li WR, Gao ZZ, et al: Activation of VEGF and ERK1/2 and improvement of urethral function by adipose-derived stem cells in a rat stress urinary incontinence model. Urology. 80:953.e1–e8. 2012. View Article : Google Scholar

12 

Kim SO, Na HS, Kwon D, Joo SY, Kim HS and Ahn Y: Bone-marrow-derived mesenchymal stem cell transplantation enhances closing pressure and leak point pressure in a female urinary incontinence rat model. Urol Int. 86:110–116. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Mitterberger M, Marksteiner R, Margreiter E, Pinggera GM, Frauscher F, Ulmer H, Fussenegger M, Bartsch G and Strasser H: Myoblast and fibroblast therapy for post-prostatectomy urinary incontinence: 1-year followup of 63 patients. J Urol. 179:226–231. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Niklason LE, Abbott W, Gao J, Klagges B, Hirschi KK, Ulubayram K, Conroy N, Jones R, Vasanawala A, Sanzgiri S and Langer R: Morphologic and mechanical characteristics of engineered bovine arteries. J Vasc Surg. 33:628–638. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P and Hedrick MH: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 13:4279–4295. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Arrigoni E, Lopa S, de Girolamo L, Stanco D and Brini AT: Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: From small to large animal models. Cell Tissue Res. 338:401–411. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Wang Y, Zhao L and Hantash BM: Support of human adipose-derived mesenchymal stem cell multipotency by a poloxamer-octapeptide hybrid hydrogel. Biomaterials. 31:5122–5130. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Wang YH, Ho ML, Chang JK, Chu HC, Lai SC and Wang GJ: Microporation is a valuable transfection method for gene expression in human adipose tissue-derived stem cells. Mol Ther. 17:302–308. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Chen B, Wang B, Zhang WJ, Zhou G, Cao Y and Liu W: In vivo tendon engineering with skeletal muscle derived cells in a mouse model. Biomaterials. 33:6086–6097. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Wang Y, Fu Q, Zhao RY and Deng CL: Muscular tubes of urethra engineered from adipose-derived stem cells and polyglycolic acid mesh in a bioreactor. Biotechnol Lett. 36:1909–1916. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Shen W, Chen X, Chen J, Yin Z, Heng BC, Chen W and Ouyang HW: The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration. Biomaterials. 31:7239–7249. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Zhu Y, Liu T, Song K, Fan X, Ma X and Cui Z: Adipose-derived stem cell: A better stem cell than BMSC. Cell Biochem Funct. 26:664–675. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Lin G, Wang G, Banie L, Ning H, Shindel AW, Fandel TM, Lue TF and Lin CS: Treatment of stress urinary incontinence with adipose tissue-derived stem cells. Cytotherapy. 12:88–95. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Wu G, Song Y, Zheng X and Jiang Z: Adipose-derived stromal cell transplantation for treatment of stress urinary incontinence. Tissue Cell. 43:246–253. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Cao D, Liu W, Wei X, Xu F, Cui L and Cao Y: In vitro tendon engineering with avian tenocytes and polyglycolic acids: A preliminary report. Tissue Eng. 12:1369–1377. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Cao Y, Rodriguez A, Vacanti M, Ibarra C, Arevalo C and Vacanti CA: Comparative study of the use of poly (glycolic acid), calcium alginate and pluronics in the engineering of autologous porcine cartilage. J Biomater Sci Polym Ed. 9:475–487. 1998. View Article : Google Scholar : PubMed/NCBI

28 

Xu ZC, Zhang WJ, Li H, Cui L, Cen L, Zhou GD, Liu W and Cao Y: Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor. Biomaterials. 29:1464–1472. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Wang C, Cen L, Yin S, Liu Q, Liu W, Cao Y and Cui L: A small diameter elastic blood vessel wall prepared under pulsatile conditions from polyglycolic acid mesh and smooth muscle cells differentiated from adipose-derived stem cells. Biomaterials. 31:621–630. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wang W, Wang X, Wang Y, Wang J, Fu Q and Shi G: Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain. Exp Ther Med 14: 1337-1342, 2017.
APA
Wang, Y., Wang, W., Wang, X., Wang, Y., Wang, J., Fu, Q., & Shi, G. (2017). Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain. Experimental and Therapeutic Medicine, 14, 1337-1342. https://doi.org/10.3892/etm.2017.4705
MLA
Wang, Y., Wang, W., Wang, X., Wang, Y., Wang, J., Fu, Q., Shi, G."Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain". Experimental and Therapeutic Medicine 14.2 (2017): 1337-1342.
Chicago
Wang, Y., Wang, W., Wang, X., Wang, Y., Wang, J., Fu, Q., Shi, G."Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1337-1342. https://doi.org/10.3892/etm.2017.4705
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wang W, Wang X, Wang Y, Wang J, Fu Q and Shi G: Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain. Exp Ther Med 14: 1337-1342, 2017.
APA
Wang, Y., Wang, W., Wang, X., Wang, Y., Wang, J., Fu, Q., & Shi, G. (2017). Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain. Experimental and Therapeutic Medicine, 14, 1337-1342. https://doi.org/10.3892/etm.2017.4705
MLA
Wang, Y., Wang, W., Wang, X., Wang, Y., Wang, J., Fu, Q., Shi, G."Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain". Experimental and Therapeutic Medicine 14.2 (2017): 1337-1342.
Chicago
Wang, Y., Wang, W., Wang, X., Wang, Y., Wang, J., Fu, Q., Shi, G."Tissue‑engineered sling with adipose‑derived stem cells under static mechanical strain". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1337-1342. https://doi.org/10.3892/etm.2017.4705
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