Open Access

Intratracheal administration of isosorbide dinitrate improves pulmonary artery pressure and ventricular remodeling in a rat model of heart failure following myocardial infarction

  • Authors:
    • Xuelian Wang
    • Qingqing Xu
    • Tianqi Li
    • Yaocong Rong
    • Weilin Hong
    • Yan Huang
    • Xingui Guo
  • View Affiliations

  • Published online on: June 28, 2017     https://doi.org/10.3892/etm.2017.4707
  • Pages: 1399-1408
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pulmonary hypertension due to left heart disease is associated with poor outcomes. This study investigated the beneficial effects of isosorbide dinitrate (ISDN) inhalation on pulmonary pressure and ventricular remodeling in a rat model of heart failure (HF) following myocardial infarction (MI). To assess the effect of ISDN on pulmonary pressure, 20 male Sprague‑Dawley (SD) rats were randomized to four groups: Normal saline (NS) 1 ml/kg, ISDN 1 mg/kg, NS 3 ml/kg or ISDN 3 mg/kg following coronary ligation. Assessments included pulmonary and systemic artery pressure alterations, lung weight/body weight and plasma nitric oxide (NO) concentration. To assess the effect of ISDN on ventricular remodeling, 30 SD rats were randomized to three groups: Sham surgery, MI‑NS (intratracheal NS 3 ml/kg for 13 days following coronary ligation), and MI‑ISDN (intratracheal ISDN 3 mg/kg for 13 days following coronary ligation). On day 15, all rats underwent echocardiogram and hemodynamic assessments. The area affected by MI was evaluated using microscopy and vascular endothelial growth factor (VEGF) expression was examined using immunohistochemistry. Plasma epinephrine, norepinephrine and brain natriuretic peptide (BNP) levels were assessed by ELISA. Intratracheal ISDN reduced pulmonary and systematic artery pressure without pulmonary edema when compared with NS. The reduction was associated with increased plasma NO levels. ISDN inhalation for 14 days reduced MI size and alleviated left and right ventricular remodeling following MI. These hemodynamic and morphological improvements were associated with decreased plasma epinephrine, norepinephrine and BNP levels, and an increased VEGF positive area at the border of MI region. In conclusion, intratracheal administration of ISDN was effective in improving ventricular remodeling and cardiac function in a rat model of HF following MI.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang X, Xu Q, Li T, Rong Y, Hong W, Huang Y and Guo X: Intratracheal administration of isosorbide dinitrate improves pulmonary artery pressure and ventricular remodeling in a rat model of heart failure following myocardial infarction. Exp Ther Med 14: 1399-1408, 2017
APA
Wang, X., Xu, Q., Li, T., Rong, Y., Hong, W., Huang, Y., & Guo, X. (2017). Intratracheal administration of isosorbide dinitrate improves pulmonary artery pressure and ventricular remodeling in a rat model of heart failure following myocardial infarction. Experimental and Therapeutic Medicine, 14, 1399-1408. https://doi.org/10.3892/etm.2017.4707
MLA
Wang, X., Xu, Q., Li, T., Rong, Y., Hong, W., Huang, Y., Guo, X."Intratracheal administration of isosorbide dinitrate improves pulmonary artery pressure and ventricular remodeling in a rat model of heart failure following myocardial infarction". Experimental and Therapeutic Medicine 14.2 (2017): 1399-1408.
Chicago
Wang, X., Xu, Q., Li, T., Rong, Y., Hong, W., Huang, Y., Guo, X."Intratracheal administration of isosorbide dinitrate improves pulmonary artery pressure and ventricular remodeling in a rat model of heart failure following myocardial infarction". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1399-1408. https://doi.org/10.3892/etm.2017.4707