Open Access

Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer

  • Authors:
    • Xianhua Meng
    • Yinghui Zhao
    • Jinyun Wang
    • Zheng Gao
    • Qingxia Geng
    • Xiaoxia Liu
  • View Affiliations

  • Published online on: August 4, 2017     https://doi.org/10.3892/etm.2017.4887
  • Pages: 2789-2794
  • Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to examine the role and underlying mechanism of miRNA‑758 (miR‑758) expression in cancer tissues, blood and cervical exfoliated cells from patients with cervical cancer. A total of 49 patients with cervical cancer and 26 healthy people for cervical cancer screening were included in the present study. The patients with cervical cancer were treated with resection, and the tumor and adjacent tissues, blood and cervical exfoliated cells were collected. The expression levels of miR‑758 and matrix extracellular phosphoglycoprotein (MEPE) mRNA in each sample were detected by reverse transcription‑quantitative polymerase chain reaction. In addition, western blot analysis was used to detect the MEPE protein in tumor tissues, while ELISA was applied to detect the MEPE protein expression in the blood and cervical exfoliated cells. Compared with the normal control, MEPE mRNA expression was upregulated in cervical cancer tissues, blood and cervical exfoliated cells. At the protein level, MEPE was also upregulated significantly in patients with cervical cancer. miR‑758 expression was decreased significantly in cervical cancer tissues, blood and cervical exfoliated cells (P<0.05), which was opposite to the trend observed for MEPE mRNA expression. Furthermore, MEPE expression was increased in the tumor tissue, blood and cervical exfoliated cells of cervical cancer patients, which was associated to the downregulated miR‑758. Therefore, miR‑758 may regulate the infiltration and invasion of cervical cancer by targeting MEPE.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Meng X, Zhao Y, Wang J, Gao Z, Geng Q and Liu X: Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer. Exp Ther Med 14: 2789-2794, 2017
APA
Meng, X., Zhao, Y., Wang, J., Gao, Z., Geng, Q., & Liu, X. (2017). Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer. Experimental and Therapeutic Medicine, 14, 2789-2794. https://doi.org/10.3892/etm.2017.4887
MLA
Meng, X., Zhao, Y., Wang, J., Gao, Z., Geng, Q., Liu, X."Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer". Experimental and Therapeutic Medicine 14.4 (2017): 2789-2794.
Chicago
Meng, X., Zhao, Y., Wang, J., Gao, Z., Geng, Q., Liu, X."Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer". Experimental and Therapeutic Medicine 14, no. 4 (2017): 2789-2794. https://doi.org/10.3892/etm.2017.4887