Expression of glutathione S‑transferase A1, a phase II drug‑metabolizing enzyme in acute hepatic injury on mice

  • Authors:
    • Xin Ma
    • Fangping Liu
    • Minmin Li
    • Zhi Li
    • Yuexia Lin
    • Rui Li
    • Changwen Li
    • Yicong Chang
    • Changwei Zhao
    • Qing Han
    • Qiong Zhou
    • Yulin Zhao
    • Dening Wang
    • Jingli Liu
  • View Affiliations

  • Published online on: August 17, 2017     https://doi.org/10.3892/etm.2017.4957
  • Pages: 3798-3804
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Abstract

In the present study, three models of acute liver injury in mice were induced via the administration of CCl4 (35 mg/kg, 24 h), acetyl‑para‑aminophenol (APAP; 200 mg/kg, 12 h) and ethanol (14 ml/kg, 8 h) to study the effect of glutathione S‑transferase A1 (GSTA1) on acute liver injury. The serum levels of alanine transaminase, aspartate transaminase and liver homogenate indicators (superoxide dismutase, glutathione and glutathione peroxidase) were significantly lower in model groups compared with the control group (P<0.01), whereas the liver homogenate indicator malondialdehyde was significantly increased (P<0.01). The expression of GSTA1 in liver was significantly decreased in the model groups compared with the control group (P<0.01). GSTA1 protein content was 3.8, 1.3 and 2.6 times lower in the CCl4, APAP and ethanol model groups, respectively. Furthermore, GSTA1 mRNA expression levels decreased by 4.9, 2.1 and 3.7 times in the CCl4, APAP and ethanol model groups, respectively. Among the three models, the injury induced by CCl4 was the most marked, followed by ethanol and finally APAP. These results suggest that GSTA1 may be released by the liver and serve as an antioxidant in the prevention of liver damage.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ma X, Liu F, Li M, Li Z, Lin Y, Li R, Li C, Chang Y, Zhao C, Han Q, Han Q, et al: Expression of glutathione S‑transferase A1, a phase II drug‑metabolizing enzyme in acute hepatic injury on mice. Exp Ther Med 14: 3798-3804, 2017
APA
Ma, X., Liu, F., Li, M., Li, Z., Lin, Y., Li, R. ... Liu, J. (2017). Expression of glutathione S‑transferase A1, a phase II drug‑metabolizing enzyme in acute hepatic injury on mice. Experimental and Therapeutic Medicine, 14, 3798-3804. https://doi.org/10.3892/etm.2017.4957
MLA
Ma, X., Liu, F., Li, M., Li, Z., Lin, Y., Li, R., Li, C., Chang, Y., Zhao, C., Han, Q., Zhou, Q., Zhao, Y., Wang, D., Liu, J."Expression of glutathione S‑transferase A1, a phase II drug‑metabolizing enzyme in acute hepatic injury on mice". Experimental and Therapeutic Medicine 14.4 (2017): 3798-3804.
Chicago
Ma, X., Liu, F., Li, M., Li, Z., Lin, Y., Li, R., Li, C., Chang, Y., Zhao, C., Han, Q., Zhou, Q., Zhao, Y., Wang, D., Liu, J."Expression of glutathione S‑transferase A1, a phase II drug‑metabolizing enzyme in acute hepatic injury on mice". Experimental and Therapeutic Medicine 14, no. 4 (2017): 3798-3804. https://doi.org/10.3892/etm.2017.4957